Goat anti human f ab 2 fragments anti igm and igg

Manufactured by Jackson ImmunoResearch

Goat anti-human F(ab′)2 fragments(anti-IgM and -IgG) are laboratory reagents produced by Jackson ImmunoResearch. These fragments are derived from the F(ab′)2 portion of goat antibodies that specifically recognize and bind to human immunoglobulin M (IgM) and immunoglobulin G (IgG) molecules.

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Lab products found in correlation

Hybridomax hybridoma growth supplement, by Gentaur (2 mentions) 7 aad, by BD (2 mentions) Ifn α, by Merck Group (1 mentions) Cd19 pe lt19, by Miltenyi Biotec (1 mentions) Cd138 allophycocyanin b b4, by Miltenyi Biotec (1 mentions) Cd38 af700, by BioLegend (1 mentions) Cd20 efluor v450 2h7, by Thermo Fisher Scientific (1 mentions) Cd27 af647 lt27, by Bio-Rad (1 mentions) Cd27 fitc m t271, by BD (1 mentions) Cd19 percp cy5.5 sj225c1, by BD (1 mentions) Cd24 fitc ml5, by BD (1 mentions) Cd95 bv421, by BioLegend (1 mentions) Cd27 bv605, by BioLegend (1 mentions) Cd3viogreen bw264 56, by Miltenyi Biotec (1 mentions) Unconjugatedgoat anti irf4 m 17, by Santa Cruz Biotechnology (1 mentions) Donkey anti goat igg af488, by Thermo Fisher Scientific (1 mentions) Human il 2, by Thermo Fisher Scientific (1 mentions) Mem amino acids solution, by Merck Group (1 mentions) Ki 67 fitc b56, by BD (1 mentions) Il 21, by Thermo Fisher Scientific (1 mentions)

2 protocols using goat anti human f ab 2 fragments anti igm and igg

Multiparametric Flow Cytometry of B Cells

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Antibodies used were CD19 PE (LT19; Miltenyi), CD138 allophycocyanin
(B-B4; Miltenyi Biotec), CD38 PE-Cy7 (HB7; BD Biosciences), CD38 AF700 (HIT2;
Biolegend), CD20 efluor V450 (2H7; eBioscience); CD27 AF647 (LT27; AbD Serotec),
CD27 FITC (M-T271), CD19 PerCP-Cy5.5 (SJ225C1; BD Biosciences), CD19 PE-Cy7
(SJ225C1; BD Biosciences), CD24 FITC(ML5; BD Biosciences), CD84 PE (CD84.1.21;
Biolegend), CD38 PerCP-Cy5.5 (HIT2; BD Biosciences), CD95 BV421 (DX2;
Biolegend), CD20 APC-H7(L27; BD Biosciences), CD27 BV605 (O323; Biolegend), CD3
VioGreen (BW264/56; Miltenyi), Ki67 FITC (B56; BD Biosciences), unconjugated
goat anti-IRF4 (M-17; Santa Cruz) and donkey anti-goat IgG AF488 (Polyclonal;
Invitrogen). Controls were isotype-matched mouse mAbs. Annexin V FITC was from
eBioscience and 7-AAD from BD Biosciences.
Reagents included human IL-2 (Roche); IL-6 (Peprotech) and IFN-α
(Sigma); IL-21 (PeproTech); goat anti-human F(ab′)₂ fragments
(anti-IgM and -IgG; Jackson Immunoresearch); HybridoMax hybridoma growth
supplement (Gentaur); Lipid Mixture 1, chemically defined (200X) and MEM Amino
Acids Solution (50X; Sigma).

Arumugakani G., Stephenson S.J., Newton D.J., Rawstron A., Emery P., Doody G.M., McGonagle D, & Tooze R.M. (2017). Early Emergence of CD19-Negative Human Antibody-Secreting Cells at the Plasmablast to Plasma Cell Transition. The Journal of Immunology Author Choice, 198(12), 4618-4628.

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In Vitro Culture of Human Lymphocytes

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Reagents utilized in the in vitro culture system include human IL-2 (Roche); IFN-γ (Sigma); IL-6, IFN-α and IL-21 (PeproTech); goat anti-human F(ab’)₂ fragments (anti-IgM and -IgG; Jackson Immunoresearch); HybridoMax hybridoma growth supplement (Gentaur); Lipid Mixture 1, chemically defined (200X) and MEM Amino Acids Solution (50X; Sigma); and PF-429242 (Tocris Bioscience).
The antibodies employed for protein detection include CREB3L2 (NBP1-88697, Novus Biologicals), ATF6 (40256; Novus Biological), ACTIN (A1978; Sigma), LC3A/B (Cell Signaling). The reagents utilized in flow cytometry assays include PE-conjugated anti-CD19 (LT19; Miltenyi Biotech), V450-conjugated anti-CD20 (2H7; E-Biosciences), PE-Cy7-conjugated anti-CD38 (HB7; BD Biosciences); APC-conjugated anti-CD138 (B-B4; Miltenyi Biotech); CountBright beads (Invitrogen) and 7-AAD (BD Biosciences).

Al-Maskari M., Care M.A., Robinson E., Cocco M., Tooze R.M, & Doody G.M. (2018). Site-1 protease function is essential for the generation of antibody secreting cells and reprogramming for secretory activity. Scientific Reports, 8, 14338.

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