PCL (Capa® 6500), 50,000 MW was from Ingevity Corporation (North Charleston, SC, USA), and PLGA was LACTEL ester-terminated, 50:50 poly(DL-lactide-co-glycolide), 127,500 MW, from Evonik Corporation (Birmingham, AL, USA). Kartogenin was from Shaanxi Dideu Medichem Co., Ltd. (Xi’an, Shaanxi, China). Solvents were from Sigma-Aldrich (St. Louis, MO, USA). A custom electrospinning apparatus was comprised mainly of a GenieTouch Syringe Pump from Kent Scientific (Torrington, CT, USA) and an ES30P-5W 0–30 kV variable power supply from Gamma High Voltage Research, Inc. (Ormond Beach, FL, USA).
Genietouch syringe pump
Manufactured by Kent Scientific
Sourced in United States
The GenieTouch syringe pump is a precision liquid handling device designed for accurate and consistent fluid delivery. It features a compact and user-friendly design with a touchscreen interface for easy operation. The pump can accommodate a wide range of syringe sizes and is capable of delivering flow rates from nanoliters to milliliters per minute.
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Lab products found in correlation
8 protocols using genietouch syringe pump
1
Electrospun Polymer Scaffold for Cartilage Repair
2
Solid-Phase Extraction of Analytes
3
Solid-Phase Extraction of Analytes
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SPE cartridges were obtained from United Chemical Technologies (UCT C8+SCX Clean Screen SPE columns; Bristol, PA, USA). All solvents used during the extraction experiments were obtained from Sigma Aldrich (Allentown, PA, USA). For the SPE pretreatment procedure, the column was first conditioned with 1.3 mL of methanol, followed by 1.3 mL of water. Then, 1-2 mL of sample was flowed through the SPE cartridge at a rate of 1 mL/min using a GenieTouch syringe pump (Kent Scientific, Torrington, CT, USA). The column was then dried by flowing air through it for 1 minute using the syringe pump in draw mode, after which analytes were eluted off the column using 100 μL of 50/50 (v/v) hexane/ethyl acetate at a flow rate of 1 mL/min. Finally, 25 μL of the collected eluate was loaded onto a SERS-active capillary and measured using SERS. The recovery efficiency of the SPE method was confirmed by gas chromatography (GC) analysis of samples with known concentration before and after extraction.
For physical separation using centrifugation, a Galaxy Mini centrifuge (VWR, Radnor, PA, USA) was employed for 5 minutes at 6000 rpm. For filtration steps, a 0.2-micron filter (Grace Davison Discovery Science, Deerfiled, IL, USA) was connected to a Luer-Lok plastic syringe (Becton-Dickinson, Franklin Lakes, NJ, USA), and the sample was passed manually through the filter.
For physical separation using centrifugation, a Galaxy Mini centrifuge (VWR, Radnor, PA, USA) was employed for 5 minutes at 6000 rpm. For filtration steps, a 0.2-micron filter (Grace Davison Discovery Science, Deerfiled, IL, USA) was connected to a Luer-Lok plastic syringe (Becton-Dickinson, Franklin Lakes, NJ, USA), and the sample was passed manually through the filter.
4
Severe and Mild Water Intoxication Protocol
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Two procedures of water intoxication by IP injection were used. Severe
WI: The infused solution was 20% BW of MilliQ water (4.8–6 ml) with 0.4
µg·kg−1 dDAVP as used in previous studies [25 (link)]. Mild WI: Based on a small number
of pilot-studies, the composition of the infused solution was defined as:
NaHCO3: 6.5 mM; KCl: 1.125 mM; and NaCl: 29.75 mM, giving a total
concentration of [Na+] of 36.25 mM. Injection volume of the mild-WI solution
was 10% BW (2.5–3 ml).
For both severe WI and mild WI the protocol consisted of: 1) a period of baseline
monitoring (40 min); 2) 2 min of IP infusion of 37°C WI solution using an automatic
infusion pump (GenieTouch Syringe pump, Kent Scientific Corp., Torrington, CT, USA); 3) a
post-infusion period of WI monitoring. Mice were monitored for a maximum of 1 h after
severe WI and 90 min after mild WI and then sacrificed by cervical dislocation.
WI: The infused solution was 20% BW of MilliQ water (4.8–6 ml) with 0.4
µg·kg−1 dDAVP as used in previous studies [25 (link)]. Mild WI: Based on a small number
of pilot-studies, the composition of the infused solution was defined as:
NaHCO3: 6.5 mM; KCl: 1.125 mM; and NaCl: 29.75 mM, giving a total
concentration of [Na+] of 36.25 mM. Injection volume of the mild-WI solution
was 10% BW (2.5–3 ml).
For both severe WI and mild WI the protocol consisted of: 1) a period of baseline
monitoring (40 min); 2) 2 min of IP infusion of 37°C WI solution using an automatic
infusion pump (GenieTouch Syringe pump, Kent Scientific Corp., Torrington, CT, USA); 3) a
post-infusion period of WI monitoring. Mice were monitored for a maximum of 1 h after
severe WI and 90 min after mild WI and then sacrificed by cervical dislocation.
5
Gelatin Channel Fabrication Protocol
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Gelatin channels were fabricated using the molding technique. A mold was prepared using a silicon container and a cylindrical plastic tube (outer diameter: 1 mm) in which 10 wt% molten gelatin in PBS was crosslinked into a tubular channel structure. To prepare 10 wt% gelatin solution, 10 g of gelatin powder (G2500, Sigma-Aldrich) was dissolved in 100 mL of PBS buffer heated to ~60 °C. Then, the gelatin solution was poured into the mold and allowed for crosslinking at 4 °C for ~30 min. After crosslinking, the plastic tube was gently slipped out and the gelatin channel block was removed from the mold. The gelatin block was then placed in a Petri dish at room temperature (~24 °C) for ~15 min before one end was connected to a syringe pump (GenieTouch Syringe Pump, Kent Scientific) via a polymer tubing (Masterflex, Cole-Parmer) for in vitro cell delivery experiments.
6
Biofilm Cultivation in 3D-Printed Flow Cells
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Biofilm cultivation was carried out in 3D-printed flow cell systems (McGlennen et al., 2023 (link)). Briefly, the flow cell was filled with sterile media (i.e., 1:10X TSB or 5% MWF), and an initial EIS measurement was collected. Subsequently, 1 mL of cell culture enrichments were injected into the flow chambers and left to adhere to the sensor surface for 2 Hrs. Following the 2 Hrs of incubation, a continuous flow of sterile media was initiated into the flow chambers at a rate of 1 µL min−1 (Genie Touch Syringe Pump, Kent Scientific, USA). Abiotic (no biofilm) control trials were used to determine the effect of treatment amendments on impedance following the same procedure.
7
Lateral Flow Test Strip Fabrication
8
Investigating the Effects of (super)FVa in Liver Trauma
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superFVa was diluted to a concentration of 0.4 or 0.8 mg/kg in 0.9% sterile saline for injection (Hosperia) and administered either as a single bolus by retro-orbital injection 5 minutes before liver laceration or by continuous infusion 30 minutes after induction of trauma/shock or liver laceration. Continuous infusion was administered via the jugular vein catheter by a GenieTouch syringe pump (Kent Scientific) at a rate of 5 µL/min for 20 minutes. Control mice received an equal volume of sterile saline. No resuscitation was administered in these animals.
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