All solvents and reagents were purchased from Merck (Merck Co., Germany) without any more purification except eugenol oxide that was synthesized from eugenol using the general procedure reviewed by Fieser and Fieser.24 EDC.HCL was prepared from Sigma (Sigma-Aldrich Co., Germany). Dimethyl sulfoxide (DMSO) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) were bought from Sigma (Sigma-Aldrich Co., Germany). The K562 cell line was obtained from the Pasteur Institute (Tehran, Iran). The cell culture medium (RPMI 1640) and penicillin/streptomycin were purchased from Gibco (Life Technologies, Paisley, Scotland). The culture plates were supplied from SPL (Seoul, South Korea). Mtb (H37Rv strain) ATCC (No.27294) was obtained from the National Reference Center (NRC) in Borstel, Germany. 7H9 medium (cat# 271310) was obtained from Becton Dickinson (BD Difco; Becton Dickinson). Rifampicin was obtained from the National Reference Center (NRC) in Borstel, Germany. Staphylococcus aureus (PTCC 1112), Escherichia coli (PTCC 1047), and Candidakefyr (ATCC 38296) were provided by the laboratory of Microbiology (Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran).
Edc hcl
Manufactured by Merck Group
Sourced in Germany, United States, Sao Tome and Principe, China
EDC·HCl is a chemical compound commonly used as a coupling agent in organic synthesis. It is a white crystalline solid that is soluble in various organic solvents. EDC·HCl is widely utilized in the preparation of peptides, esters, and amides, facilitating the formation of stable carbon-nitrogen or carbon-oxygen bonds.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
7h9 medium, by BD (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium hydride, by Merck Group (1 mentions)
Allyl bromide, by Merck Group (1 mentions)
Tetraethylene glycol, by Merck Group (1 mentions)
Sodium ascorbate, by Merck Group (1 mentions)
P toluenesulfonyl chloride, by Merck Group (1 mentions)
Anhydrous tetrahydrofuran thf, by Merck Group (1 mentions)
1 thioglycerol, by Merck Group (1 mentions)
Anhydrous n n dimethylformamide dmf, by Merck Group (1 mentions)
N n diisopropylethylamine dipea, by Merck Group (1 mentions)
Cy5 mono nhs ester, by Cytiva (1 mentions)
Anhydrous dcm, by Merck Group (1 mentions)
2 2 dimethoxy 2 phenylacetophenone, by Merck Group (1 mentions)
Copper sulfate pentahydrate, by Merck Group (1 mentions)
Trifluoracetic acid, by Merck Group (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
12 protocols using edc hcl
1
Protocol for Chemical Synthesis and Antimicrobial Evaluation
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All solvents and reagents were purchased from Merck (Merck Co., Germany) without any more purification except eugenol oxide that was synthesized from eugenol using the general procedure reviewed by Fieser and Fieser.24 EDC.HCL was prepared from Sigma (Sigma-Aldrich Co., Germany). Dimethyl sulfoxide (DMSO) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) were bought from Sigma (Sigma-Aldrich Co., Germany). The K562 cell line was obtained from the Pasteur Institute (Tehran, Iran). The cell culture medium (RPMI 1640) and penicillin/streptomycin were purchased from Gibco (Life Technologies, Paisley, Scotland). The culture plates were supplied from SPL (Seoul, South Korea). Mtb (H37Rv strain) ATCC (No.27294) was obtained from the National Reference Center (NRC) in Borstel, Germany. 7H9 medium (cat# 271310) was obtained from Becton Dickinson (BD Difco; Becton Dickinson). Rifampicin was obtained from the National Reference Center (NRC) in Borstel, Germany. Staphylococcus aureus (PTCC 1112), Escherichia coli (PTCC 1047), and Candidakefyr (ATCC 38296) were provided by the laboratory of Microbiology (Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran).
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All solvents and reagents were purchased from Merck (Merck Co., Germany) without any more purification except eugenol oxide that was synthesized from eugenol using the general procedure reviewed by Fieser and Fieser.24 EDC.HCL was prepared from Sigma (Sigma-Aldrich Co., Germany). Dimethyl sulfoxide (DMSO) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) were bought from Sigma (Sigma-Aldrich Co., Germany). The K562 cell line was obtained from the Pasteur Institute (Tehran, Iran). The cell culture medium (RPMI 1640) and penicillin/streptomycin were purchased from Gibco (Life Technologies, Paisley, Scotland). The culture plates were supplied from SPL (Seoul, South Korea). Mtb (H37Rv strain) ATCC (No.27294) was obtained from the National Reference Center (NRC) in Borstel, Germany. 7H9 medium (cat# 271310) was obtained from Becton Dickinson (BD Difco; Becton Dickinson). Rifampicin was obtained from the National Reference Center (NRC) in Borstel, Germany. Staphylococcus aureus (PTCC 1112), Escherichia coli (PTCC 1047), and Candidakefyr (ATCC 38296) were provided by the laboratory of Microbiology (Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran).
2
Synthesis and Functionalization of Clickable Probes
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All the reagents were used as received unless otherwise stated. Propargyl bromide solution (80 wt % in toluene), allyl bromide, sodium hydride (60% dispersion in mineral oil), 2,2-dimethoxy-2-phenylacetophenone, 1-thioglycerol, EDC.HCl, DMAP, N,N′-diisopropylethylamine (DIPEA), p-toluenesulfonyl chloride, tetraethylene glycol, TFA, γ-(Boc-amino)butyric acid (BOC-GABA-OH), copper sulfate pentahydrate, sodium ascorbate, anhydrous DCM, anhydrous tetrahydrofuran (THF), and anhydrous N,N′-dimethylformamide (DMF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cy5-mono-NHS ester and fluorescein isothiocyanate (FITC) were purchased from Amersham Biosciences–GE Healthcare. All other American Chemical Society (ACS) grade solvents were from Fisher Scientific. Deuterated solvents dimethylsulfoxide (DMSO-d6), water (D2O), methanol (CD3OD), and chloroform (CDCl3) were purchased from Cambridge Isotope Laboratories Inc. (Andover, MA). Dialysis membrane (molecular weight cutoff of 1000 Da) was obtained from Spectrum Laboratories Inc. (Rancho Dominguez, CA, USA).
3
SAH Conjugation with BSA
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A 3.8-mg quantity of SAH (Sigma) was dissolved in 1.5 mL of PBS. Then, 10 mg of EDC HCl (Sigma) and 4.5 mg of NHS were added, and the mixture was stirred at room temperature for 24 h. We then dissolved 12.9 mg of BSA in PBS. The SAH was added to the BSA solution, and the mixture was incubated at 4 °C in the dark overnight.
4
Glycoprotein Sample Preparation for Mass Spec
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All chemicals were purchased from Sigma-Aldrich unless otherwise noted. Snap-cap spin columns (SCSC), Aminolink resin, graphitized-active carbon column, HPLC-grade water, and acetonitrile (ACN) were purchased from Fisher Scientific. Ethylenediamine (EDA), p-toluidine (pT), 1-hydroxybenzotriazole hydrate (HBot), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) (liquid), EDC·HCl (powder), urea, ammonium bicarbonate, dithiothreitol (DTT), iodoacetamide (IAA), 2,5-dihydroxybenzoic acid (DHB), N,N′-dimethylaniline (DMA), formic acid (FA), DMSO, hydrochloric acid (HCl), maltoheptaose (DP7), phosphate-buffered saline (PBS, 1×), sodium cyanoborohydride (NaCNBH3), sodium carbonate, sodium citrate, Tris-HCl, and trifluoracetic acid (TFA) were from Sigma-Aldrich. NaCl (5 M) was from ChemCruz Biochemicals. Peptide-N-glycosidase F (PNGase F), denaturing buffer (10×; 400-mM DTT and 5% sodium dodecyl sulfate), and reaction buffer (G7; 10×; 500-mM sodium phosphate, pH 7.5) were purchased from New England BioLabs (Ipswich, MA). Sequencing-grade modified trypsin was purchased from Promega Corporation (Madison, WI). HILIC SPE chromatography was prepared as follows: add Empty SPE (solid-phase extraction) frits to Grace Alltech Extract-Clean empty reservoir (1.5 mL; Fisher Scientific), load 500-μL TSKgel Amide-80 (Sigma-Aldrich), and cap resin using Empty SPE frits.
5
Amine-Functionalized Borosilicate Glass Slides
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Borosilicate glass slides (10 mm × 10 mm × 0.5 mm) were purchased from SCHOTT AG. AziGrip4 amine coating was performed by SuSoS, Switzerland. CaCl2, EDC.HCl, HOBt and MES hydrate were bought from Sigma Aldrich. Succinic anhydride was purchased from TCI. Sodium acetate was purchased from Acros Organic. MgCl2 was bought from Roth, NaCl from Fisher Scientific and KCl from Alfa Aesar. PBS buffer was purchased from Gibco. SSC 20X buffer was purchased from Alfa Aesar. Linear oligonucleotides were purchased from GenScript with HPLC purification. Molecular beacons were bought from Integrated DNA technologies. Coatings were performed with a MSC-100 Cooling Thermoshaker Incubator form Labgene Scientific, Switzerland. All coatings were performed with Milli-Q ultrapure water. Fluorescence measurements of Cy3-modified oligos were carried out with a Synergy H1 multiplate reader from BioTek, at λexc = 532 nm, λem = 568 nm. XPS: X-ray Photoelectron Spectroscopy measurements were carried out on an Axis Supra (Kratos Analytical) using the monochromated Ka X-ray line of an Aluminium anode at the X-Ray Diffraction and Surface Analytics Platform (EPFL–ISIC–XRDSAP, Sion, Switzerland). The pass energy was set to 40eV with a step size of 0.15eV. Charge neutralization was done using a low energy electron gun and the spectra were referenced at 284.8eV using the aliphatic component of the C 1s orbital.
6
Alginate Microspheres Chemical Modification
7
Nanohybrid Formulation for Targeted Delivery
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APN-NH2 was provided by ChemeGen (Los Angeles, CA). Distearoylphosphatidylethanolamine–polyethylene glycol (MW∼5 kDa)-COOH (DSPE-PEG5000-COOH, DSPE-PEG) was purchased from Ponsure Biotech (Shanghai, China). Soyabean lecithin (SL) in injection grade was supplied by Shanghai Taiwei Pharmaceutical Co., Ltd. (Shanghai, China). Baicalin, TPP, EDC·HCl, and NHS were obtained from Sigma-Aldrich (St. Louis, MO).
8
Gelatin Methacryloyl-Based Bioactive Hydrogel
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Gelatin Methacryloyl (GelMA, EFL-GM-30) was purchased from EFL Technology Company (Suzhou, China). Imidazole, EDC-HCL, sodium hexametaphosphate, sodium hydroxide (NaOH) anhydrous cerium chloride, alcohol, and 3 % H2O2 are provided by Sigma Aldrich. Isopropyl myristate and dehydrated sorbitol oleate (Span 80) were purchased from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). All reagents can be used without further purification.
9
Synthesis of Eight-arm PEG Amine Conjugate
10
Multiplex Serological Assay for Porcine Viruses
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The HP-PRRSV strain HuN4, HP-PRRSV vaccine (HuN4-F112, TJM-F92, JXA1-R), classical PRRSV vaccine CH-1R and MLV, NADC30-like PRRSV SDHS53, NADC34-like PRRSV TZJ1341, European PRRSV (VP046 BIS and DV) and recombinant SDHS53 N protein (240 μg/ml) used in this study were retained at the HVRI of the CAAS. Porcine parvovirus (PPV), porcine circovirus type 2 (PCV-2), classical swine fever virus (CSFV), transmissible gastroenteritis virus (TGEV), porcine pseudorabies virus (PRV), and porcine epidemic diarrhea virus (PEDV) were collected at the HVRI. Latex microspheres (diameter = 300 nm) were purchased from Hangzhou Xiaoque Technology Co., Ltd. (Hangzhou, China). MES, EDC·HCl, and NHS were procured from Sigma (Sigma, St. Louis, MO, United States). Nitrocellulose (NC) membranes (Millipore 180) were obtained from Millipore (Billerica, United States). Polyester membranes were purchased from Pall Corporation (NY, United States), and absorbent paper and PVC sheets were procured from Shanghai Jinbiao Biotechnology Co., Ltd. (Shanghai, China).
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