Fluoromax 2 spectrofluorometer

Manufactured by Horiba

Sourced in United States

The Fluoromax-2 is a spectrofluorometer manufactured by Horiba. It is a versatile instrument designed for measuring the fluorescence properties of various samples. The Fluoromax-2 is capable of performing excitation and emission scans, as well as time-resolved fluorescence measurements. The instrument uses a xenon lamp as the light source and can detect fluorescence signals across a wide range of wavelengths.

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Lab products found in correlation

Lambda 35 uv visible spectrometer, by PerkinElmer (1 mentions) Cary 3e spectrophotometer, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Spectrofluorometer (35 citations) Fluoromax spectrofluorometer (22 citations) FluoroMax-2 (22 citations)

4 protocols using fluoromax 2 spectrofluorometer

Fluorometric Monitoring of Mitochondrial NADH and Oxygen Consumption

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NADH (excitation 340 nm, emission 460 nm) fluorescence was measured in a Fluoromax-2 spectrofluorometer (Horiba Jobin Yvon, Inc., Edison, NJ, U.S.A).
Oxygen consumption by mitochondrial suspensions was measured with a polarographic Rank Brothers oxygen electrode with Perspex chamber (Rank Brothers, Cambridge, U.K.) with a magnetic stirrer. In experiments where fluorescence and oxygen consumption were monitored simultaneously, the fluorometric 1 cm × 1 cm cuvette was equipped with a magnetic stirrer, a stopper with an injection port and a miniature oxygen electrode (MI-730, Microelectrodes, Inc., Bedford, NH, U.S.A.).

Cameron A.R., Logie L., Patel K., Erhardt S., Bacon S., Middleton P., Harthill J., Forteath C., Coats J.T., Kerr C., Curry H., Stewart D., Sakamoto K., Repiščák P., Paterson M.J., Hassinen I., McDougall G, & Rena G. (2017). Metformin selectively targets redox control of complex I energy transduction. Redox Biology, 14, 187-197.

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Fluorescence Spectroscopy of Dissolved Organic Matter

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Absorbance spectra were measured from 200 to 700 nm at 1-nm intervals, with a Lambda 35 UV–visible spectrometer (Perkin Elmer, Waltham, MA, USA). Samples were measured in a 1-cm quartz cuvette and distilled water was used as a blank measurement.
Excitation–emission matrices (EEMs) were collected with a FluoroMax-2 spectrofluorometer (Horiba Scientific, Edison, NJ, USA), using a 1-cm quartz cuvette. Excitation wavelengths (λEx) spanned from 250 to 445 nm in 5-nm increments, whereas emission wavelengths (λEm) ranged from 300 to 600 nm at increments of 4 nm. Excitation and emission slit widths were set to 5 nm and the integration time was 0.1 s. Blank subtraction, correction of EEMs and calibration to Raman units was carried out according to Murphy et al. (2010) (link). Four individual fluorescing components in the EEMs were identified and validated with parallel factor (PARAFAC) analysis, using the MATLAB and Statistics Toolbox (R2013a; The MathWorks, Inc., Natick, MA, USA) in combination with the DOMFluor toolbox (Stedmon and Bro, 2008 ). The components were derived from the EEMs of 95 samples and their fluorescence characteristics are depicted as insets in Figure 3b.

Logue J.B., Stedmon C.A., Kellerman A.M., Nielsen N.J., Andersson A.F., Laudon H., Lindström E.S, & Kritzberg E.S. (2015). Experimental insights into the importance of aquatic bacterial community composition to the degradation of dissolved organic matter. The ISME Journal, 10(3), 533-545.

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Absorption and Fluorescence Spectroscopy of Peptides

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Dry peptides were dissolved in ultrapure deionized water, PB, TFE, ethanol or DMF to a concentration of 8 µM. Baseline-corrected absorption spectra were recorded at r.t. in single-beam mode on a Cary 4000 UV–visible (UV-VIS) spectrophotometer (Varian) using 500 µl volume, 10 mm light-path length quartz glass cells (Hellma). Fluorescence measurements were performed immediately after UV-VIS measurements on a FluoroMax2 spectrofluorometer (HORIBA Jobin Yvon) equipped with a thermostated cell compartment without cell exchange (excitation wavelength varied; typically, 1 nm slits were used for both excitation and emission channels). The data were processed and analyzed in OriginPro 2020b.

Afonin S., Koniev S., Préau L., Takamiya M., Strizhak A.V., Babii O., Hrebonkin A., Pivovarenko V.G., Dathe M., le Noble F., Rastegar S., Strähle U., Ulrich A.S, & Komarov I.V. (2021). In Vivo Behavior of the Antibacterial Peptide Cyclo[RRRWFW], Explored Using a 3-Hydroxychromone-Derived Fluorescent Amino Acid. Frontiers in Chemistry, 9, 688446.

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Spectroscopic Characterization of Compounds

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UVvis absorption spectra were recorded in a Varian Cary 3E spectrophotometer. Fluorescence excitation and emission spectra were obtained in a Jobin-Yvon Spex Fluoromax-2 spectrofluorometer, with correction for instrumental factors by means of a reference photodiode and correction files supplied by the manufacturer. Fluorescence quantum yields were measured using quinine sulfate (< 3  10 -5 mol dm -3
) in aqueous HClO 4 (1.0 mol dm -3
) as standard ( = 0.546). 32, 33 To improve the accuracy of the quantum yields measurements, log-normal functions were fitted to the emission bands to evaluate the band areas. 34 All experiments were carried out at 20 ºC. Fluorescence decays were measured by the timecorrelated single-photon counting technique in an Edinburgh Instruments LifeSpec-ps time-resolved spectrometer equipped with a sub-nanosecond pulsed LED from PicoQuant as the excitation source (308 nm). The reconvolution analysis software supplied by the manufacturer was employed.

Rodríguez-Prieto F., Corbelle C.C., Fernández B., Pedro J.A., Ríos Rodríguez M.C, & Mosquera M. (2017). Fluorescence quenching of the N-methylquinolinium cation by pairs of water or alcohol molecules. Physical chemistry chemical physics : PCCP, 20(1).

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