CD spectra were recorded on a Chirascan plus ACD (Applied Photophysics Ltd, England) [35 ]. HR1P and HR2P were dissolved in buffer (0.1 M KCl, 0.05 M KH2PO4, pH 7.2) at a final concentration of 10 μM. Briefly, HR1P was incubated with PBS or UA-30 (20 μM) at 25 °C for 30 min, followed by addition of HR2P (10 μM). After further incubation at 25 °C for 30 min, the CD wave scans were measured from 190 to 260 nm at 4 °C with the bandwidth of 2 nm and the step size of 1 nm.
Chirascan plus acd
Manufactured by Applied Photophysics
Sourced in United Kingdom
The Chirascan plus ACD is a circular dichroism (CD) spectrophotometer designed and manufactured by Applied Photophysics. It is a versatile instrument that can be used to analyze the chirality and conformation of various biomolecules, such as proteins, peptides, and nucleic acids. The Chirascan plus ACD provides accurate and reliable measurements of CD spectra, allowing users to characterize the secondary structure and folding of these molecules.
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Lab products found in correlation
3 protocols using chirascan plus acd
1
Circular Dichroism Spectroscopy of Protein Interactions
2
Analysis of Polysaccharide Secondary Structures
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The secondary structures of GPs and their Zn (II) derivatives were analyzed using a Chirascan plus ACD spectropolarimeter (Applied Photophysics, Leatherhead, UK). Two polysaccharides were dissolved in 5 mM citrate buffer solution and then scanned in the range of 180–400 nm at a constant rate (100 nm/min) and bandwidth (1.0 nm). Three scans were conducted and averaged for each sample.
3
Circular Dichroism Spectroscopy of Protein Interactions
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CD spectra were recorded on a Chirascan plus ACD (Applied Photophysics ltd, England). HR1P and HR2P were dissolved in buffer (0.1 M KCl, 0.05 M KH2PO4, pH 7.2) at a final concentration of 10 μM. Briefly, HR1P was incubated with PBS or BA-4 (20 μM) at 25 ℃ for 30 min, followed by addition of HR2P (10 μM). After further incubation at 25 ℃ for 30 min, the CD wave scans were measured from 190 to 260 nm at 4 ℃ with the bandwidth of 2 nm and the step size of 1 nm [18] (link), [19] (link).
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