For SPECT imaging in vitro, the apoptotic and normal C6 cells cultured under the same conditions described above were incubated with the 99mTc-duramycin-Au DENPs or 99mTc-Au DENPs at different radiodoses (25, 50, 100, 200, and 400 μCi/mL) for 4 h. Then, the cells were treated under the above conditions and scanned according to our previous work (Li et al., 2016 (link)). Similarly, apoptotic and normal C6 cells were incubated with fresh medium containing PBS, duramycin-Au DENPs, or Au DENPs at different Au concentrations (20, 40, 60, 80, and 100 μM) for 3 h. After appropriate treatment according to our previous work (Zhou et al., 2018 (link)), the cells were imaged by a GE Discovery STE PET/CT system with a tube voltage of 100 kV, an electrical current of 220 mA, and a slice thickness of 1.25 mm.
Discovery ste pet ct system
Manufactured by GE Healthcare
Sourced in United States
The Discovery STE PET/CT system is a medical imaging device designed for diagnostic purposes. It combines positron emission tomography (PET) and computed tomography (CT) technologies to capture detailed images of the body's internal structures and function. The system is used in healthcare settings to assist medical professionals in the diagnosis and management of various conditions.
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7 protocols using discovery ste pet ct system
1
SPECT Imaging of Apoptotic C6 Cells
2
FDG PET/CT Imaging Protocol for Myocardial Viability
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After Tc-99m sestamibi myocardial perfusion SPECT, F-18 fluorodeoxyglucose (FDG) cardiac positron emission tomography (PET)/computed tomography (CT) (FDG PET) was done (Fig. 2 and 3 ). Imaging was performed on a Discovery STE PET/CT system (GE medical systems, Milwaukee, WI, USA). Preparation was performed using simplified glucose/insulin loading (20 g of dextrose intravenously with simultaneous intravenous and subcutaneous insulin to adjust blood glucose to 5 mM/L) (15 (link)). PET/CT acquisitions for the heart were started at 40 min after the injection of 7.4 MBq per kilogram of body weight. CT images were acquired using parameters with a peak voltage of 140 keV, a tube current of 20 mA×16 sec, a rotation time of 1.0 sec, a field of view of 50 cm and a slice thickness of 3.27 mm. Immediately following the CT acquisition, the PET data were acquired in the same anatomic locations in the 2D mode with acquisition time of 15 min. The CT data were used for attenuation correction and the images were reconstructed using a conventional iterative algorithm (OSEM). A Xeleris workstation (GE medical systems) providing multiplanar reformatted images was used for image display and analysis.
3
18F-FDG PET/CT Imaging for Brain Analysis
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Patients fasted for at least 6 hours prior to examination. Before radiopharmaceutical injection, blood glucose was measured (< 130 mg/dL in all cases). Each subject was intravenously injected with approximately 185 MBq of 18F-FDG 60 minutes before the scan. 18F-FDG PET/CT scans were then acquired by a Discovery ST-E PET/CT System (GE Medical Systems) combining a helical multislice CT scanner and a designed Bismuth germanate (BGO) block detector PET tomograph, in 3D modality with a total axial field of view of 30 cm and no interplane gap space. 18F-FDG PET/CT images were acquired through 2 sequential scans: CT brain scan (thickness, 3.75 mm; 140 kV; 60-80 mA/s) and PET brain scan [Field of View (FOV) of 30 cm, transaxial]. The PET scan was initiated immediately after the CT examination in order to use CT data for the attenuation correction of the PET data. 3D data were reconstructed using a 3D-OSEM algorithm (VUE-point) with the corrections (random, scatter, attenuation) incorporated into the iterative process. The parameters were: number of subsets 28; number of iteration 4. Data were collected in 128 × 128 matrices with a reconstructed voxel of 1.33 x 1.33 x 2.00 mm. Finally, 18F-FDG PET/CT images were fused with contrast enhanced 3D SPGR T1-weighted images using the Integrated Registration software on the Advantage image processing workstation (GE Medical Systems).
4
Standardized PET/CT Imaging Protocol
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All patients underwent 18F-fluoro-2-dexoxy-D-glucose (18F-FDG) PET/CT imaging on a Discovery STE PET/CT system (GE Healthcare, USA), consisting of a 16-detector-row CT scanner. The 18F-FDG PET/CT imaging agent was produced by the PET/CT center at the Tumor Hospital of Shanxi Province using 18F produced with a MINItrace drug synthesis system (GE Healthcare, USA). Eventually, imaging agents were synthesized using a Trace Lab FXN multifunctional composite device. The chemical purity of the drug was greater than 90%.
Patients fasted for at least 4 hrs before intravenous administration of 18F-FDG (5–6MBq/kg) to ensure a serum glucose level below 11 mmol/L. PET/CT scanning was acquired at 1 hr after 18F-FDG administration. Transmission data were acquired using a low-dose CT scan (120 kV, 180 mA, a 512×512 matrix, 3.75-mm slice thickness), and the scanning range was from vertex to the upper segment of the femur. PET images were acquired by the 3D model, and the scanning range was the same as for CT. Six to eight bed-boards were collected according to the different heights of patients, with one bed-scanning taking 3 mins. The images were reconstructed using a conventional iterative algorithm (OSEM). Multiplanar reformed PET and CT images were analyzed frame by frame on a Xeleris workstation (GE Healthcare, USA).
Patients fasted for at least 4 hrs before intravenous administration of 18F-FDG (5–6MBq/kg) to ensure a serum glucose level below 11 mmol/L. PET/CT scanning was acquired at 1 hr after 18F-FDG administration. Transmission data were acquired using a low-dose CT scan (120 kV, 180 mA, a 512×512 matrix, 3.75-mm slice thickness), and the scanning range was from vertex to the upper segment of the femur. PET images were acquired by the 3D model, and the scanning range was the same as for CT. Six to eight bed-boards were collected according to the different heights of patients, with one bed-scanning taking 3 mins. The images were reconstructed using a conventional iterative algorithm (OSEM). Multiplanar reformed PET and CT images were analyzed frame by frame on a Xeleris workstation (GE Healthcare, USA).
5
PET/CT Imaging of 18F-FDG in Cancer
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A Discovery STE PET/CT system (GE Healthcare, Waukesha, WI, USA) was used to perform PET/CT. The PET/CT center of Shanxi Cancer Hospital provided 18F-FDG with a radiochemical purity of >95%. Patients fasted for >6 h before examination. The fasting blood glucose concentration was checked and confirmed to be <11 mmol/L before examination. Image scanning was performed 60 min after intravenous injection of 18F-FDG (0.12–0.15 mCi/kg). PET was performed in the 3-dimensional mode using 3.75 mm per slice. CT was performed using the following parameters: 120 kV, 200 mA, 0.8 s/lap, and 22.5 mm/s bed speed. The images were immediately obtained from the top of the skull to the upper femur (6–8 bed positions and 3 min per bed position). The PET images were reconstructed and attenuation-corrected by CT images. The images were then observed on an Xeleris Workstation (GE Healthcare) for assessment. Two experienced nuclear medicine radiologists reviewed all PET/CT data independently and reached a consensus on the results. The reviewers were blinded to the EGFR mutational status.
6
Standardized PET Imaging Protocol
7
Standardized Technique for FP-CIT PET/CT Imaging
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PET/CT imaging was performed using a Discovery STE PET/CT system (GE Healthcare, Chicago, IL, USA), comprising a bismuth germanate full PET scanner and a 16-detector-row CT scanner. Intravenous injection of 185 MBq F-18 FP-CIT (Future-Chem Co., Ltd., Pusan, Korea) was administered on the PET/CT scanner table, and a PET scan was acquired for the first 5 min after injection as an early perfusion image. Routine dopamine transporter (DAT) image acquisition was started 3 h after the injection, and PET scan was performed for 20 min. For acquisition of images, patient was placed on the table and head was fixed using bandage. Emission PET data were acquired in the 3-dimensional mode after a low-dose CT scan. CT scans were obtained without contrast enhancement and were used for attenuation correction. Images were checked for movement, but motion correction was not applied. The images were reconstructed using an ordered-subset expec-tation maximum conventional iterative algorithm with 35 subsets/4 iterations, a 256 × 256 matrix (slice thickness of 3.27 mm), and a 3.2-mm Gaussian post reconstruction filter (VUE point, GE Healthcare).
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