For transmission electron microscopy (TEM) analysis, 5 μL of sample solution was loaded onto a copper-grid coated carbon film. After the sample had adsorbed for 1 min, the grid was washed with a drop of purified water and then negatively stained with 1% uranyl acetate for 1 min. Excess stain solution was removed using a piece of filter paper, and the grid was air-dried. The sample was imaged at a focus between 0.8 and 1.5 μm using a JEM-1400 Plus transmission electron microscope (JEOL Ltd., Tokyo, Japan) equipped with a Lab6 gun and operating at 120 kV. Images were recorded using an UltraScan OneView CMOS camera (Gatan, Pleasanton, CA, USA).
Ultrascan oneview cmos camera
Manufactured by Ametek
Sourced in United States
The UltraScan OneView CMOS camera is a high-performance lab equipment product designed for scientific and industrial applications. It features a CMOS imaging sensor that captures high-resolution images and video. The camera offers advanced functionality, such as fast frame rates and adjustable exposure times, to suit a variety of laboratory and research needs.
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2 protocols using ultrascan oneview cmos camera
1
TEM Analysis of Nanoparticle Samples
2
Exosome Characterization by TEM and NTA
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Exosome samples were imaged under a JEM-1400 Plus transmission electron microscope (JEOL Ltd., Tokyo, Japan) at an under focus of 0.8–1.5 μm and recorded using an UltraScan OneView CMOS camera (Gatan, Pleasanton, CA, USA). Samples were prepared by loading 5 μL solution onto an EM grid covered with glow-discharged continuous carbon film. The grid was washed with deionized water after 1 min and stained with 1% uranyl acetate for 1 min. After removal of staining solution using filter paper, the grid was dried completely in open air.
The size distribution of particles was determined by nanoparticle tracking analysis (NTA), which assesses the combined properties of light scattering and Brownian motion. Isolated EVs in liquid were diluted in 1 mL phosphate-buffered saline (PBS; Lonza), and visualized and counted by a Nanosight instrument (Malvern Instrument, Worcestershire, UK) at a temperature of 25 °C using a 488 nm laser.
The size distribution of particles was determined by nanoparticle tracking analysis (NTA), which assesses the combined properties of light scattering and Brownian motion. Isolated EVs in liquid were diluted in 1 mL phosphate-buffered saline (PBS; Lonza), and visualized and counted by a Nanosight instrument (Malvern Instrument, Worcestershire, UK) at a temperature of 25 °C using a 488 nm laser.
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