α amylase bacillus amyloliquefaciens

Soluble starch, D-glucose, α-glucosidase (type V from rice), p-nitrophenyl α-D-glucopyranoside, acarbose, 3,5-dinitrosalicylic acid (DNS), Folin-Ciocalteu reagent, gallic acid, quercetin, 6-hydroxy-2-5-7-8-tetramethylchroman-2-carboxylic acid (Trolox), 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulphate, 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH), sodium fluorescein, 2,4,6-tripyridyl-s-triazine (TPTZ), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, USA. α-amylase (Bacillus amyloliquefaciens) was purchased from Roche Diagnostics, USA. All the other chemicals and reagents were of analytical grade. All the analyses were carried out using high-throughput 96-well microplate readers (SpectraMax Plus384, Molecular Devices, USA and SPECTRAmax-Gemini EM, Molecular Devices Inc, USA).

Soluble starch, bovine serum albumin (BSA), D-glucose, α-glucosidase (type V from rice), p-nitrophenyl α-D-glucopyranoside, acarbose, trichloroacetic acid (TCA), acetylcholinesterase (AChE) from electric eel (Type-VI-S), butyrylcholinesterase (BChE) from horse serum, acetylthiocholine, butyrylthiocholine, 5,5′-dithio-bis-(2-nitrobenzoic) acid (DTNB), methylglyoxal (MGO), 3,5-dinitrosalicylic acid (DNS), dimethyl sulfoxide (DMSO), galantamine, rutin, cyanidin chloride, and ammonium iron(III) sulfate dodecahydrate were purchased from Sigma-Aldrich, USA. α-Amylase (Bacillus amyloliquefaciens) was purchased from Roche Diagnostics, USA. All the other chemicals and reagents were of analytical grade.

Folin-Ciocalteu reagent (FC Reagent), gallic acid, quercetin, acarbose, 6-hydroxy-2-5-7-8-tetramethylchroman-2carboxylic acid (Trolox), ethylenediaminetetraacetic acid (EDTA), 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2, 2-azino-bis (3ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulphate, 2, 2′-azobis (2-amidinopropane) dihydrochloride (AAPH), sodium fluorescein, 2,4,6 tripyridyl-s-triazine (TPTZ), and α-amylase (Bacillus amyloliquefaciens) were purchased from Roche Diagnostics (USA), and 3,5-dinitrosalycylic acid, 4, 4′-disulfonic acid sodium salt (ferrozine), soluble starch, bovine serum albumin (BSA), D-glucose, and trichloro-acetic acid (TCA) were purchased from Sigma-Aldrich (USA). All other chemicals used for the preparation of buffers and solvents were in the analytical grade. Spectrophotometric assays were conducted using 96-well microplate readers (Spectra Max Plus384, Molecular Devices, USA, and SPECTRAmax-Gemini EM, Molecular Devices Inc., USA). The mineral analysis was done using microwave digester CEM Mar 5, USA, using the Easy Prep digestion program and ICP-MS (Agilent 7900, USA).