Human GC cell lines including HGC-27, AGS, SGC-7901, BGC-823, NCI-N87 and human normal gastric mucosal cells GSE-1 were purchased from Chinese Academy of Sciences Affiliated Cell Resource Center of Shanghai Institute of Life Sciences (Shanghai, China). HGC-27, BGC-823, SGC-7901 and GSE-1 cells were cultured in 90% RPMI 1640 medium (Sigma-Aldrich, USA) supplemented with 10% FBS (Gibco, USA); AGS cells were cultured in 90% F12K medium (Sigma-Aldrich, USA) supplemented with 10% FBS (Gibco, USA); NCI-N87 cells were cultured in 88% RPMI 1640 medium (Sigma-Aldrich, USA) supplemented with 10% FBS (Gibco, USA), 1% glutamax (Invitrogen, USA), and 1% sodium pyruvate (Invitrogen, USA). All these cell lines were incubated in a humidified incubator under 95% air and 5% CO2 condition at 37°C.
Nci n87
Manufactured by Merck Group
Sourced in United States
The NCI-N87 is a human gastric carcinoma cell line derived from a poorly differentiated adenocarcinoma of the stomach. It is commonly used in cell culture research and assays.
Automatically generated - may contain errors
Lab products found in correlation
Nci n87, by American Type Culture Collection (6 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Merck Group (3 mentions)
Hgc 27, by Merck Group (2 mentions)
Rpmi 1640, by Merck Group (2 mentions)
Snu 16, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions)
Penicillin, by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Bgc 823, by Merck Group (1 mentions)
Sgc 7901, by Merck Group (1 mentions)
F12k medium, by Merck Group (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Sk br 3, by Merck Group (1 mentions)
Au 565, by Merck Group (1 mentions)
Hcc1954, by Merck Group (1 mentions)
11 protocols using nci n87
1
Culturing Human Gastric Cancer Cell Lines
2
Establishing Cisplatin-Resistant Gastric Cancer Cell Lines
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The human gastric cancer cell lines, NCI-N87 (RRIDs: CVCL_1603) and AGS (RRIDs: CVCL_0139), were purchased from ATCC (American Type Culture Collection, USA). The cells were cultured in DMEM/F12 (Invitrogen, CA, USA) medium that supplemented with 10% (v/v) of heat inactivated FBS (Gibco, USA) at 37 °C with 5% CO2. To generate cisplatin resistant GC cell lines, NCI-N87 and AGS cells were treated with gradually increasing concentrations of cisplatin (Sigma-Aldrich, MS, USA) for about two months until the cells could grow in the medium supplemented with 1000 nM cisplatin. The resistant cell lines were named AGS Cis-R and NCI-N87 Cis-R.
3
Characterization of Breast Cancer Cell Lines
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Cell lines were obtained from ATCC (HCC2218, HCC1954, HCC1419, ZR-75-30, AU565, NCI-2170, BT-474, SK-BR-3, NCI-N87, SKOV3, MCF7), Sigma (OE-19), DSMZ (JIMT-1), Cedarlane (ZR-75-1) and AddexBio (MDA-MB-175-VII, MDA-MB-468). Cell lines were authenticated by supplier and were used from source and expanded for a maximum of 20 passages. Cell lines were routinely spot check tested for mycoplasma, all tests were negative. Cell line supplier and catalog numbers are provided in Supplementary Table 14 . Trastuzumab (Herceptin®) and pertuzumab (Perjeta®) were purchased from Crown Bio and Xentech, respectively. Zanidatamab was prepared according to standard manufacturing procedures (CMC Bio). A non-specific IgG1 Ab that binds to respiratory syncytial virus (RSV) protein F, palivizumab, was used as a negative control (neg. control or NC) and was produced under standard expression and purification conditions. Human complement serum, baby rabbit complement serum and pooled murine complement serum were obtained from Cedarlane. Mouse strain serum (male and female Balb/c, male and female CB17 SCID) was collected under protocols that were approved by the Animal Care Committee at University of British Columbia.
4
Taxol and AhR Agonist Treatment of Gastric Cancer Cells
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Two human gastric cancer cell lines, AGS and NCI-N87, obtained from the American Type Culture Collection were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientifc, Inc.) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, Inc.) in a humidified incubator with 5% CO2 and 95% air at 37°C. For treatment, Tax was obtained from Shanghai Huicheng Technology, Ltd. The AGS and NCI-N87 cells were treated with increasing concentrations of Tax (1, 3, 10, 30 and 100 µM) for 48 h. In addition, the aryl hydrocarbon receptor (AhR) agonist SB203580 (10 µM; Sigma-Aldrich; Merck KGaA) was applied for further treatment.
5
Characterization of Gastric Cancer Cell Lines
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Three human gastric cancer cell lines were used in the present study. NCI-N87 and SNU-16 cells were purchased from American Type Culture Collection (ATCC) (Rockville, MD, USA). SCH cells were purchased from Japan Health Sciences Foundation (Osaka, Japan). NCI-N87, SNU-16, and SCH were maintained in RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10 % (v/v) fetal bovine serum at 37 °C under 5 % CO2. The histological type of each cell line is shown in Table 1 .
Histological type and HER2 status of three gastric tumor tissues
| Tumor name | Histological typea | HER2 IHC score | HER2/CEP17 ratio (FISH) |
|---|---|---|---|
| NCI-N87 | Differentiated/intestinal-type epithelial carcinoma | 3+ | 8.4 [11 (link)] |
| SCH | Undifferentiated-/diffuse-type choriocarcinoma | 2+ | 2.3 |
| SNU-16 | Undifferentiated-/diffuse-type epithelial carcinoma | 1+ | 1.4 [11 (link)] |
aHistological type was described according to the information from each cell line distributor
6
Gastric Cancer Cell Line Bile Acid Exposure
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For our purposes, we required well-established, acid-stable gastric cancer cell lines with comparable levels of c-Myc expression (10 (link)). Accordingly, we purchased AGS (ATCC® CRL-1739™) and NCI-N87 (ATCC® CRL-5822™) cell lines from the American Type Culture Collection (Manassas, VA, USA). These gastric cancer cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) (GIBCO Invitrogen) containing 4.5 mg/l glucose, 100 mg/l streptomycin, and 2 mM L-glutamine supplemented with 10% fetal bovine serum (FBS) (GIBCO Invitrogen). They were maintained at 37°C under a humidified 5% CO2 atmosphere in a CO2 incubator (Sanyo). Solutions of bile acids (Sigma-Aldrich) were prepared using appropriate solvents according to the manufacturer's protocols (Table SI ). AGS and NCI-N87 cells were cultured in the growth medium for 24 h and then transferred to fresh, serum-free medium containing 100 µM of a bile acid for 48 h, with the bile acid being cholic acid (CA; Sigma-Aldrich, C9377), chenodeoxycholic acid (CDCA; Sigma-Aldrichl, C1129), taurocholic acid (TCA; Sigma-Aldrich, T4009), or glycochenodeoxycholic acid (GCDCA; Sigma-Aldrich, G0759). Afterward, we extracted the total RNA and total protein from the cells.
7
Culturing Human Stomach Cancer Cell Lines
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A panel of six HSCCLs were used in this study. Of the six HSCCLs examined in this study FU97, MKN74 and MKN1 were purchased from Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan); AGS, HGC-27 and NCI-N87 were purchased from Culture Collections (Public Health England, Porton Down, UK). MKN1, MKN74 and NCI-N87 were cultured with Roswell Park Memorial Institute-1640 medium (RPMI-1640) (Sigma, UK), AGS was cultured in F12-HAM (Sigma, UK), HGC-27 was cultured in Eagle’s minimum essential medium (EMEM) (Sigma, UK), and FU97 was cultured in Dulbecco’s modified Eagle medium (DMEM) (Sigma, UK). All media were supplemented with 10% fetal bovine serum (FBS) and the antibiotics, penicillin (50 U/mL), streptomycin (0.05 mg/mL) and neomycin (0.1 mg/mL, Sigma, UK). RPMI-1640 and EMEM were also supplemented with glutamine (Sigma, UK), whereas DMEM was supplemented with insulin (Sigma, UK).
8
Culturing Human Gastric Cancer Cell Lines
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The human GC cell line ACC-201 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures. The human GC cell line CRL-5822 (NCI-N87) was obtained from the American Type Culture Collection (ATCC). Both cancer cell lines were cultured in RPMI1640 medium supplemented with 10% FBS (Sigma-Aldrich) and antibiotics (100 IU/mL of penicillin and 100 μg/mL of streptomycin (Sigma-Aldrich) in a humidified 5% CO2 atmosphere at 37 °C (ACC-201 and NCI-N87).
9
Demethylation Induces DAL-1 Expression
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The human GC cell lines AGS, NCI-N87, KATOIII, SNU-1, SNU-5, SNU-16, and Hs746T and the human embryonic kidney cell line HEK-293T were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The human GC cell line HGC-27 was obtained from the Cell Resources Center of Shanghai Life Sciences, Chinese Academy of Sciences (Shanghai, China). The cells were authenticated by the Beijing Microread Genetics (Beijing, China) using short tandem repeat (STR) analysis. All cells were routinely maintained. AGS, NCI-N87, and KATOIII cells (1 × 106/mL) were grown in either the presence or absence of the demethylating agent 5-Aza-2′-CdR (5 μmol/L, Sigma, St. Louis, MO, USA) for 3 to 5 days, and then checked with the expression of DAL-1.
10
Culturing NCI-N87 Gastric Cancer Cells
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We purchased Human GC cell line NCI-N87 from American Type Culture Collection. NCI-N87 cells were placed in Dulbecco's Modified Eagle Medium (DMEM; Sigma, USA) with the addition of 10% (v/v) heat-inactivated fetal bovine serum (FBS, Sigma, USA), 2 mM glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin at a RESEARCH humidified surrounding of 37°C, 5% carbon dioxide (CO 2 ).
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