5-Carboxyfluorescein-labeled peptides, 5-carboxyfluorescein-Ahx-DPPLHS-pT-AI-NH2,23 (link) 5-carboxyfluorescein-Ahx-GPMQTS-pT-PKNG, and 5-carboxyfluorescein-Ahx-GPLATS-pT-PKNG45 (link) (final concentrations: 10 nM) were incubated with corresponding Plk1, Plk2 and Plk3-PBDs (PBD concentration was used at EC85) purified from E. coli in a binding buffer containing 10 mM Tris-Cl (pH 8.0), 1 mM EDTA, 50 mM NaCl, and 0.01% Nonidet P-40. Binding reactions were carried out in the presence of testing peptides at the indicated concentrations. Fluorescence polarization was measured 30 min after mixing all components in the 384-well format using Molecular Devices Spectra Max Paradigm with a Multi-Mode Microplate Detection platform. Data acquired from three independent experiments were plotted using GraphPad Prism 7.
Multi mode microplate detection platform
Manufactured by Molecular Devices
Sourced in United States
The Multi-Mode Microplate Detection platform is a versatile lab equipment designed for high-performance detection and analysis of microplate samples. It provides a range of detection modes, including absorbance, fluorescence, and luminescence, enabling comprehensive analysis of various biological and chemical assays.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using multi mode microplate detection platform
1
Fluorescent-Labeled Peptide Binding Assay
2
Evaluating HepG2 Cell Viability with PA and PBL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MTT analysis method was adopted to analyzed cell viability. Briefly, HepG2 cells were add to 96 wells plate (3500 cells/well). After 24 h of incubation, HepG2 cells were exposed to a series concentration of PA (0.1, 0.2, 0.3, 0.5, 0.8, 1 mM) for 48 h. To detect the effect of PBL on cell viability, HepG2 cells were exposed to a range of PBL concentrations (0.78, 1.59, 3.18, 6.25, 12.5, 25 µg/mL) for 48 h. Moreover, we focused on the impact of PBL on the survival rate of HepG2 cells induced by PA, cells were cultured with 300 µM PA and PBL from 0.78 to 25 µg/mL at 48 h. To add 10 µL MTT solution to each well and continue incubation for 4 h. The medium was removed, and 200 µL of DMSO was added to each well, then the absorbance was measured at 570 nm using Multi-Mode Microplate Detection Platform (Molecular Devices, Sunnyvale, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!