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Bamhi xhol

Manufactured by Addgene

BamHI/Xhol is a restriction enzyme that recognizes and cleaves DNA sequences. It has dual specificity, cutting DNA at sequences recognized by both BamHI and Xhol restriction enzymes.

Automatically generated - may contain errors

2 protocols using bamhi xhol

1

Construction of AAV Plasmids

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Standard molecular cloning methods were used to construct AAV plasmids. pAAV-CBA-ConFon-EYFP-WPRE was constructed by subcloning BamHI/Xhol fragment from Addgene #55650 into AAV backbone containing CBA promoter (cytomegalovirus/chicken beta-actin hybrid promoter). The CBA promoter, also named CAG promoter, was initially cloned from Addgene #11160. The pAAV-EF1a-DIO-EGFP-WPRE was constructed on the backbone of pAAV-Ef1a-DIO eNpHR 3.0-EYFP (Addgene #26966). The pAAV-EF1a-fDIO-tdtomato-WPRE was made based on pAAV-Ef1a-fDIO-EYFP (Addgene #55641) by replacing EYFP with tdTomato.
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2

Construction of AAV Plasmids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Standard molecular cloning methods were used to construct AAV plasmids. pAAV-CBA-ConFon-EYFP-WPRE was constructed by subcloning BamHI/Xhol fragment from Addgene #55650 into AAV backbone containing CBA promoter (cytomegalovirus/chicken beta-actin hybrid promoter). The CBA promoter, also named CAG promoter, was initially cloned from Addgene #11160. The pAAV-EF1a-DIO-EGFP-WPRE was constructed on the backbone of pAAV-Ef1a-DIO eNpHR 3.0-EYFP (Addgene #26966). The pAAV-EF1a-fDIO-tdtomato-WPRE was made based on pAAV-Ef1a-fDIO-EYFP (Addgene #55641) by replacing EYFP with tdTomato.
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