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Lumina 2

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The Lumina II is a high-performance luminescence microplate reader developed by PerkinElmer. It is designed to accurately and reliably measure luminescent signals in a variety of microplate-based assays.

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Lab products found in correlation

Living image software, by PerkinElmer (2 mentions) D luciferin, by GoldBio (2 mentions) D luciferin, by PerkinElmer (2 mentions) Living image 4, by PerkinElmer (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Matrigel, by BD (1 mentions) Living image v 4, by PerkinElmer (1 mentions) 70 μm nylon cell strainer, by BD (1 mentions) Living image software 4, by PerkinElmer (1 mentions) Fh535, by Merck Group (1 mentions) Balb c nude mice, by Orient Bio (1 mentions) Vivoglo luciferin, by Promega (1 mentions) Living image software version 3, by PerkinElmer (1 mentions) P1043, by Promega (1 mentions)

Spelling variants of this product

IVIS Lumina II (170 citations) IVIS Lumina II system (33 citations) IVIS Lumina Series II (6 citations) Xenogen IVIS Lumina II system (5 citations) Caliper IVIS Lumina II (5 citations) Lumina II IVIS imager (4 citations) IVIS Lumina II Quantitative Fluorescent and Bioluminescent Imaging (3 citations) Caliper Lumina II (2 citations) Lumina II Living Image 4.0 software (2 citations) Caliper IVIS Lumina II system (2 citations)

21 protocols using lumina 2

1

Biolayer Interferometry Imaging of Tumors

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Biolayer interferometry was performed once a week until the end of the study using an in vivo imaging system‐Lumina II (Perkin Elmer), generating a pseudocolored image representing light intensity and superimposed over a grayscale reference image. Each mouse was intraperitoneally injected with 150 mg/kg luciferin potassium salt (115144‐35‐9; Fanbo). Ten minutes later, the mice were anesthetized using 1.5% isoflurane. To maintain body temperature, mice were placed on a thermostatically controlled heating pad (37°C) during imaging. Acquisition binning and duration were set according to tumor activity. Signal intensity was quantified as the total flux (photons/s) within regions of interest drawn manually around the tumor area using Living Image 4.0 software (Perkin Elmer). Signals from both prone and supine positions were obtained.
Li M., Zhang W., Yang X., Liu H., Cao L., Li W., Wang L., Zhang G, & Gao R. (2019). Downregulation of HNRNPK in human cancer cells inhibits lung metastasis. Animal Models and Experimental Medicine, 2(4), 291-296.
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2

In Vivo Bioluminescence Imaging

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After 10 min postintraperitoneal injection of luciferin (3 mg/20 g bodyweight, Keyuandi, Shanghai, China), the intensity of in vivo biofluorescence of each mouse was evaluated and the pictures were taken using the Lumina II in vivo imaging system (Perkin Elmer, Fremont, CA, USA).
Xiao M., Bian Q., Lao Y., Yi J., Sun X., Sun X, & Yang J. (2021). SENP3 loss promotes M2 macrophage polarization and breast cancer progression. Molecular Oncology, 16(4), 1026-1044.
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3

Fluorescently Labeled Designer EV Biodistribution

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All animals were randomly grouped in cages. For this procedure animals were anesthetized using 5% inhaled isoflurane to induce and maintained with 1–2% isoflurane while conducting the procedure (according to the weight of the animal). The animals were positioned on an intubation stand with adjustable head position (Kent Scientific). A 23 μL bolus of fluorescently labeled mGluR8 designer EVs (1.6 × 108 EVs/μL) resuspended in DMEM plain media were gently delivered though each nostril of the animal. Respiratory rate/effort was constantly monitored throughout the procedure to minimize discomfort. 24 hours after designer EV treatment the animals were euthanized and all major organs (brain, heart, liver, lungs, spleen, kidneys, and pancreas) were collected, briefly washed in PBS (Thermo Fisher Scientific), and then transferred into a 2% FBS (Gibco, Thermo Fisher Scientific) solution in sterile PBS (Thermo Fisher Scientific) at 4°C. All organs were immediately imaged using the bioluminescent in vivo imaging system (IVIS) Lumina II (PerkinElmer Inc). Images of all organs were captured at 535nm excitation emission and 1 second exposure time (using stages A and B).
Ortega-Pineda L., Sunyecz A., Salazar-Puerta A.I., Rincon-Benavides M.A., Alzate-Correa D., Anaparthi A.L., Guilfoyle E., Mezache L., Struckman H.L., Duarte-Sanmiguel S., Deng B., McComb D.W., Dodd D., Lawrence W.R., Moore J., Zhang J., Reátegui E., Veeraraghavan R., Nelson M.T., Gallego-Perez D, & Higuita-Castro N. (2022). Designer extracellular vesicles modulate pro-neuronal cell responses and improve intracranial retention. Advanced healthcare materials, 11(5), e2100805.
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4

Apoptosis Evaluation with ApoPep-1

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The histone-1 targeting peptide (ApoPep-1) has been shown to be useful for evaluating apoptosis.9 (link) It was conjugated with a red fluorescent dye (AHR-553, BioActs, Incheon, South Korea), and the dye-conjugated peptide (excitation and emission wavelengths of 561 nm and 589 nm, respectively) was intravenously injected (100 nmol) 2 hours before the rats were killed. One-mm-thick myocardial rings were imaged using a fluorescence imaging system (Lumina II, Perkin-Elmer, Waltham, Massachusetts) at 535 nm excitation and 583 nm emission.
Choi H., Han J.H., Lim S.Y., Lee I., Cho Y.S., Chun E.J, & Lee W.W. (2017). Imaging of Myocardial Ischemia–Reperfusion Injury Using Sodium [18F]Fluoride Positron Emission Tomography/Computed Tomography in Rats and Humans. Molecular imaging, 16, 1536012117704767.
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5

Biodistribution of DiR-labeled MSC-EVs

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DiR-labeled MSC-EVs were injected in BALB/c mice of 6–8 weeks of age via intravenous (IV), intratracheal (IT) or intranasal (IN) administration and monitored over time using an IVIS® Imaging System LUMINA II (PerkinElmer, Waltham, MA, USA). Imaging was performed after injection, at 3 and 24 h after EV administration. After 24 h, the mice were sacrificed, the organs were resected, and images of the organs were captured. Ventral and dorsal images were obtained for each animal and quantified through the region of interest (ROI). We measured the fluorescence as radiance (p/s/cm2/sr) in the live animals and excised organs, using the Living Image 3.2 software program (PerkinElmer).
Tolomeo A.M., Zuccolotto G., Malvicini R., De Lazzari G., Penna A., Franco C., Caicci F., Magarotto F., Quarta S., Pozzobon M., Rosato A., Muraca M, & Collino F. (2023). Biodistribution of Intratracheal, Intranasal, and Intravenous Injections of Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles in a Mouse Model for Drug Delivery Studies. Pharmaceutics, 15(2), 548.
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6

Modeling Chronic Pneumonia and NSCLC

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Here, 4‐wk‐old female BALB/c athymic nude mice (SLAC Laboratory Animal Co. Ltd, Shanghai, China) were maintained in a specific room that was climate controlled, with food and water, and on a 12 h/12 h, light/dark cycle. Mice were allowed to adapt to the housing facilities for 7 d before any experimental procedures were begun. All protocols were approved by the animal use and care committee of Soochow University. Chronic pneumonia was established by injection of porcine pancreatic elastase (PPE). PPE (Solarbio) was dissolved in 0.9% normal saline. The mice were anesthetized and an incision of the neck skin was made. Then 3.75 units of PPE in 50 μL solvent was injected into the main trachea.
At 2 wk after PPE injection, NSCLC cells (5 × 106) in 100 µL Matrigel (Becton Dickinson) were injected into the left lungs of nude mice. At 5 wk later, the mice were anesthetized and given d‐luciferin in PBS. At 20 min after d‐luciferin injection, bioluminescence was imaged with a charge‐coupled device camera (IVIS; Lumina II, PerkinElmer). Then, the lung and tumor tissues were stripped, formalin fixed, and paraffin embedded.
Liang Z., Ge X., Xu M., Qin H., Wu M., Shen M., Zhang Y., Liu X., Chen K., Li W., Duan W, & Qin S. (2021). Tumor‐associated macrophages promote the metastasis and growth of non‐small‐cell lung cancer cells through NF‐κB/PP2Ac‐positive feedback loop. Cancer Science, 112(6), 2140-2157.
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7

Labeling CytoC/aNGs with Cy5.5 for In Vivo Imaging

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CytoC/aNGs was labeled with Cy5.5. In brief, CytoC/aNGs dissolved in the sodium bicarbonate buffer (pH 8.5) was added with Cy5.5 NHS ester (1:1, mol/mol). After reaction at 4 °C for 8 h, Cy5.5-CytoC/aNGs was obtained after repeated buffer exchange with PBS. The tumor-bearing mice were intratumorally administrated with Cy5.5-CytoC/aNGs/Gel or Cy5.5-CytoC/aNGs (5 mg/kg, 0.1 mL). The mice were imaged by in vivo imaging system (PerkinElmer, Lumina II, USA).
Jiang T., Ma Y., Xu X., Ji Q., Feng M., Cheng C., Feng Y., He B, & Mo R. (2020). Enzyme-instructed hybrid nanogel/nanofiber oligopeptide hydrogel for localized protein delivery. Acta Pharmaceutica Sinica. B, 11(7), 2070-2079.
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8

In Vivo Biodistribution Monitoring

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To monitor the targeting capability of the PMNG, an in vivo imaging system (IVIS) (Lumina II, PerkinElmer) was used. At days 1, 7, 14, 21, and 28 after IV injection, three mice were euthanized. The hindlimbs, heart, liver, lung, spleen, and kidneys were harvested. Fluorescent images were taken with an excitation of 535 nm and a DsRed emission filter to collect the fluorescence signal of rhodamine. The fluorescent intensity at each limb was calculated using PerkinElmer Living Image software.49 (link)
Dang Y., Gao N., Niu H., Guan Y., Fan Z, & Guan J. (2021). Targeted Delivery of a Matrix Metalloproteinases-2 Specific Inhibitor Using Multifunctional Nanogels to Attenuate Ischemic Skeletal Muscle Degeneration and Promote Revascularization. ACS applied materials & interfaces, 13(5), 5907-5918.
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9

Murine Pleural Carcinomatosis Model

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Experimental pleural carcinomatosis was induced by pleural delivery of 1.5 × 105 murine cancer cells, 106 human cancer cells or 3 × 106 HEK293T cells. MPE models and splenectomy have been described elsewhere5 (link)6 (link)7 (link). For bioluminescence imaging, mice were serially imaged on a Xenogen Lumina II and data were analysed using Living Image v.4.2 (Perkin-Elmer, Waltham, MA), after delivery of 1 mg intravenous D-luciferin (Gold Biotechnology, St Louis, MO) by retro-orbital injection. For splenocyte give-back, spleens were removed under sterile conditions from CAG.Luc.eGFP donors (n=3 per group), 13 days after intrapleural injection with saline or tumour cells. Single-cell suspensions were prepared by passing spleens through 70 μm nylon cell strainers (BD Biosciences, Bedford, MA), followed by delivery of 100 μl saline containing 5 × 106 splenocytes to splenectomized hosts.
Αgalioti T., Giannou A.D., Krontira A.C., Kanellakis N.I., Kati D., Vreka M., Pepe M., Spella Μ., Lilis I., Zazara D.E., Nikolouli E., Spiropoulou N., Papadakis A., Papadia K., Voulgaridis A., Harokopos V., Stamou P., Meiners S., Eickelberg O., Snyder L.A., Antimisiaris S.G., Kardamakis D., Psallidas I., Μarazioti A, & Stathopoulos G.T. (2017). Mutant KRAS promotes malignant pleural effusion formation. Nature Communications, 8, 15205.
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10

Luminescence-based Parasite Quantification

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At 20-26 h after reinvasion into new RBCs (i.e. 48 hours after the start of the assay), 180 µL of NF54/ap2g-re9h parasite culture was transferred from each well to a black 96-well plate (Greiner CELLSTAR microplate 655086, F-bottom, black) and incubated with 20 µL D-Luciferin (3.75 mg/mL in PBS) (Perkin Elmer, catalog# 122799) for 5-10 min at room temperature. Luminescence intensities were then measured using an intra-vital imaging system (Perkin Elmer, Lumina II) by exposing the plates for 3 min. Luminescence counts per well were determined by fitting a grid over the plate using the software Living Image (version 4.7.2).
Thommen B.T., Passecker A., Buser T., Hitz E., Voss T.S, & Brancucci N.M. (2022). Revisiting the Effect of Pharmaceuticals on Transmission Stage Formation in the Malaria Parasite Plasmodium falciparum. Frontiers in Cellular and Infection Microbiology, 12, 802341.
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