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2 protocols using ultima gold scintillation cocktail

1

Antioxidant Assays with Viper Venom

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Sinapic acid, gallic acid (GA), thiobarbituric acid (TBA), Sephadex (G25, G50, and G75), CM-Sephadex-25, 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), Ultima Gold Scintillation Cocktail, and dimethyl sulfoxide (DMSO) areproducts of Sigma-Aldrich, St. Louis, MO, USA. 14C-oleic acid was procured from Perkin Elmer Life Sciences Inc. in Boston, MA, USA. The venom of Viper russelli was purchased from Irula Cooperative Society Ltd., Tamil Nadu, India. All reagents and chemicals used in the investigation were of superior quality.
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2

Characterization of Anti-inflammatory Compounds

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Sephadex G-25, −50 and −75, CM sephadex C25 Corosolic acid, Oleanolic acid and Lipopolysaccharide (#L2018), Ultima Gold Scintillation Cocktail, Bovine serum albumin (BSA), were procured from Sigma-Aldrich, USA.14C-oleic acid obtained from Perkin Elmer Life Sciences Inc. Boston. Mouse Anti-Human IL-6 (Cat No: 340527) was from B.D Biosciences. Six-well cell culture plates from BioLite - Thermo. The PC3 Cell line was obtained from the national centre for cell science (NCCS), Pune, the cell culture medium (RPMI 1640, #AL028A), Fetal Bovine Serum (#RM10432) and dulbecco’s phosphate buffered saline (D-PBS) (#TL1006) were procured from HiMedia Laboratories, Mumbai, India. Ethylenediaminetetraacetic acid (EDTA) and dimethyl sulphoxide (DMSO) were acquired from SRL (Sisco Research Laboratories), India. The solvents and chemicals utilised in the entire investigation were standard laboratory grade.
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