A 5‐ethynyl‐2'‐deoxyuridine (EdU) kit (C0071L) was purchased from Beyotime Biotechnology. The EdU assay was carried out according to the manufacturer's protocol. Cells were seeded in 24‐well plates at a density of 5,000 cells per well. EdU was added to the culture medium. After 2 h, the cells were washed and fixed with 4% paraformaldehyde for 15 min. Then, the cells were incubated with Click Additive Solution for 30 min. After three washes, the nuclei were stained with DAPI for 10 min. Ten random images of each sample were captured using a confocal microscope (SP5, Leica, Wetzlar, Germany).
5 ethynyl 2 deoxyuridine edu kit
Manufactured by Beyotime
Sourced in China
5-ethynyl-2′-deoxyuridine (EdU) is a nucleoside analog that can be incorporated into DNA during active DNA synthesis. The EdU kit provides a method for detecting and analyzing cellular proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Bio-Rad (1 mentions)
Cell counting kit 8 cck 8 assay, by Beyotime (1 mentions)
Live dead staining kit, by Thermo Fisher Scientific (1 mentions)
Imagej, by Leica camera (1 mentions)
Acrylamide, by Maclin Biochemical Technology (1 mentions)
Streptozotocin (stz), by Solarbio (1 mentions)
Sodium citrate buffer, by Solarbio (1 mentions)
Trichloromethane, by Sinopharm (1 mentions)
Mmp 9 antibody, by Proteintech (1 mentions)
Cell counting kit 8 (cck8), by Biosharp (1 mentions)
Isopropyl alcohol, by Sinopharm (1 mentions)
Potassium persulfate, by Sinopharm (1 mentions)
Fluorescence microscope, by Olympus (1 mentions)
6 protocols using 5 ethynyl 2 deoxyuridine edu kit
1
EdU Assay for Cell Proliferation
2
Proliferation of A549 and H1299 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The proliferation of the A549 and H1299 cells was measured by a Cell Counting Kit-8 (CCK8) assay (Beyotime, SHH, CHN) and 5-ethynyl-2′-deoxyuridine (EdU) kit (Beyotime, SHH, CHN). After COL5A2 siRNA or EPHB2 siRNA treatment, A549 and H1299 cells were seeded in 96-well plates with 5000 cells and 200 µL DMEM per well for 48 h and then incubated with the EdU kit for 3 h following the manufacturer’s protocol. Alternatively, the cells were cultured for 0–72 h and incubated with the CCK-8 kit following the manufacturer’s instructions for 2 h. A microplate reader (Bio-Rad, Berkeley, CA, USA) was used to detect the absorbance of the cells at 450 nm.
3
Cytotoxicity and Proliferation Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Live/Dead staining Kit (Invitrogen, USA) was applied to determine the cytotoxicity. The cells were incubated with a staining solution and washed with phosphate buffer solution (PBS) (KeyGen Biotech, China). The 5-ethynyl-2′-deoxyuridine (EdU) Kit (Beyotime Biotechnology, China) was applied to evaluate proliferation. Pictures taken by confocal laser scanning microscope (CLSM) (Leica, Germany) and cells counted by ImageJ (Version 5.0, USA).
4
Polymer Microsphere Fabrication Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acrylamide (AM) was supplied by Macklin (Shanghai, China). Potassium persulfate, trichloromethane and isopropyl alcohol were purchased from Sinopharm Chemical Reagent (Shanghai, China). Potassium sucrose octasulfate was purchased from Avito (Shanghai, China). Polyvinyl alcohol (PVA), methyl methacrylate (MMA), Span-80, divinylbenzene (DVB) and all other chemicals were purchased from Aladdin (Shanghai, China). Cell counting kit 8 was purchased from Biosharp (Wuhan, China). Live/dead staining kits, sodium citrate buffer and streptozotocin were purchased from Solarbio (Beijing, China). The activated partial thromboplastin time assay kit and prothrombin time assay kit were purchased from Rayto (Shenzhen, China). The 5-ethynyl-2’-deoxyuridine (EdU) kit was provided by Beyotime (Shanghai, China). The MMP-9 protein reagent for the in vitro cell assay was purchased from Novoprotein (Suzhou, China). The transforming growth factor-β (TGF-β) antibody was provided by Boster (Wuhan, China). The vascular endothelial growth factor (VEGF) antibody and epidermal growth factor (EGF) antibody were purchased from ABclonal (Wuhan, China). The MMP-9 antibody was purchased from Proteintech (Wuhan, China). The platelet endothelial cell adhesion molecule-1 (CD31) antibody was provided by Abcam (Shanghai, China).
5
EdU Proliferation Assay in BGC-823 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This assay was performed by using an 5-ethynyl-2'-deoxyuridine (EdU) kit (Beyotime, Shanghai, China). Briefly, BGC-823 cells (2 × 104 cells/well) seeded in the 24-well plate were cultured with different formulations (PBS, Apa, CS-6, Apa&CS-6 and HA-Apa-Lip@PB-CS-6 NPs) for 44 h. Next, all cells were incubated with EdU for another 4 h, fixed with 4% paraformaldehyde for 20 min, and permeated with 0.3% Triton X-100 for 15 min. After incubation with the click reaction mixture and Hoechst 33342, cell images were recorded using CLSM.
6
Cell Proliferation Assay using EdU
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was assessed using a 5-ethynyl-2′-deoxyuridine (EdU) kit (Beyotime Institute of Biotechnology, Cat# C0071S). Briefly, cells were incubated with EdU (10 µM) for 4 hours, fixed with 4% paraformaldehyde for 15 minutes, and permeated with 0.3% Triton X-100 for another 15 minutes, and the EdU signal was then visualized according to the manufacturer’s instructions. 4,6-Diamidino-2-phenylindole was used for nuclear staining. The cells were visualized at a magnification of 100× using a fluorescence microscope (Olympus, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!