Mouse monoclonal caspase 3 antibody

BKM120 was purchased from Selleck Chemicals (Houston, TX). BKM120 were dissolved in DMSO and stored at -80°C. We purchased MEK inhibitors, PD0325901 and U0126, from LC laboratories (Woburn, MA) and Selleck Chemicals (Houston, TX), respectively. Chloroquine (CQ), polyclonal and monoclonal actin antibodies were purchased from Sigma Chemical Co. (St. Louis, MO). Mouse monoclonal ERK1/2, p-ERK1/2 (Thr202/Tyr204), PARP, Akt, p-Akt (S473) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Mouse monoclonal caspase-3 antibody was purchased from Imgenex (San Diego, CA). Rabbit polyclonal LC3 antibody (NB100-2220) was purchased from Novus Biologicals, Inc. (Littleton, CO).

(+)-JQ1 was purchased from ApexBio (Houston, TX). OTX015 and MS436 were purchased from Cayman Chemical (Ann Arbor, MI). Soluble recombinant human TRAIL was purchased from PeproTech, Inc. (Rocky Hill, NJ). MG132, cycloheximide (CHX) and PFI-1 were purchased from Sigma Chemical Co. (St. Louis, MO). Monoclonal anti-FLIP antibody (NF6) was obtained from Alexis Biochemicals (San Diego, CA). Mouse monoclonal caspase-8, PARP, survivin and c-Myc antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Mouse monoclonal caspase-3 antibody was purchased from Imgenex (San Diego, CA). Rabbit polyclonal DR5 antibody was obtained from ProSci Inc. (Poway, CA). Mouse monoclonal DR4 antibody (B-N28) was purchased from Diaclone (Stamford, CT). Rabbit polyclonal Mcl-1 and Bcl-X_L/S and mouse monoclonal Bcl-2 antibody were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Tubulin and GAPDH antibodies were purchased from Sigma Chemical Co. and Trevigen Inc. (Gaithersburg, MD), respectively.