Clonogenic growth assays were performed in 6-well cell culture plates with 2,000 cells per well, as we previously described.41 (link), 43 (link) All wells were pre-coated with 0.5% agarose as the bottom layer whereas the top layer is consisted of 0.3% agarose and tumor cells. After 6–8 weeks, colonies were stained with crystal violet blue solution (Sigma) for 1 hr and counted under a microscope. Triplicate wells were used for each cell line and three independent experiments were performed.
Crystal violet blue solution
Manufactured by Merck Group
Sourced in United Kingdom
Crystal violet blue solution is a laboratory reagent used for various staining and labeling applications in biological and microscopy research. It consists of a water-soluble cationic dye that binds to negatively charged cellular components, allowing for the visualization and identification of specific structures or microorganisms. The solution provides a consistent and reliable staining performance for a wide range of scientific investigations.
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Lab products found in correlation
4 protocols using crystal violet blue solution
1
Clonogenic Growth Assay Protocol
2
Clonogenic Growth Assay Protocol
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Clonogenic growth assays were performed in 6-well cell culture plates with 2,000 cells per well, as we previously described.41 (link), 43 (link) All wells were pre-coated with 0.5% agarose as the bottom layer whereas the top layer is consisted of 0.3% agarose and tumor cells. After 6–8 weeks, colonies were stained with crystal violet blue solution (Sigma) for 1 hr and counted under a microscope. Triplicate wells were used for each cell line and three independent experiments were performed.
3
Colony-Forming Assay for miR-215-5p
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The colony-forming assay was performed using six-well plates and tumor cells stably transfected with miR-215-5p or control vector were seeded in a concentration of 250 cells per well. After 12–14 days, colonies were stained with crystal violet blue solution (Sigma-Aldrich). Plates were scanned, and colonies counted and analyzed by GelCount (Oxford Optronix, Abingdon, UK).
4
Quantifying Colony Formation Assay
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Colony-forming assay was performed using six-well plates pre-coated with 0.75% agarose as the bottom layer, whereas the top layer consisted of 0.35% agarose and tumor cells transfected with miR-215 mimics or control oligonucleotide. After 12–14 days, colonies were stained with crystal violet blue solution (Sigma-Aldrich) and scanned by GelCount (Oxford Optronix, Abingdon, UK). The data were analyzed using ImageJ software (Wayne Rasband, NIH, MD, USA).
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