Nicolet 6700 thermo scientific ftir spectrometer

¹H and ¹³C NMR spectra of glucan were recorded at 27°C on a Bruker 5 mm broadband, with a spectrometer (Bruker Avance 600 MHz (German)), which operated at 126 MHz for ¹³C NMR and 600 MHz for ¹H NMR. The sample (20 mg) was dissolved in D₂O (99.8%, 0.5 mL), lyophilized and re-dissolved again in D₂O (0.5 mL). The sample was finally centrifuged to remove excessive un-dissolved sample before analysis. All the experiments were recorded using standard Bruker software. FT-IR spectra were recorded on a Nicolet 6700 Thermo Scientific FT-IR spectrometer (USA) with detector (DTGS) in a range of 4000–400 cm⁻¹. The sample measured on KBr discs as a film.

The structural characterization of the composite microspheres was determined through FTIR by using a Nicolet 6700 Thermo Scientific FTIR spectrometer (Waltham, MA, USA). Measurements were carried out in absorbance mode at wavenumber range from 4000 to 400 cm⁻¹ at a resolution of 4 cm⁻¹. X-ray diffraction (XRD) was performed by using an X-ray diffractometer D8 advance Brucker (Billerica, MA, USA) in the range of 10° to 80° 2Ɵ with a step size of 0.01° and dwell time of 1° per minute.

N,N′-dicyclohexylcarbodiimide (DCC) and O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate (TBTU) were obtained from Acros Organics (Geel, Belgium). Additionally, 30% hydrogen peroxide, potassium tetrachlorido platinate(II), N-hydroxysuccinimide, and 1-pyrenebutyric acid were purchased from Sigma-Aldrich, Taufkirchen, Germany. A Nicolet 6700 Thermo Scientific FTIR spectrometer was used for recording the IR spectra in KBr, whereas a Bruker Avance III HD spectrometer (500.13 MHz for ¹H and 125 MHz for ¹³C NMR) was used to obtain the NMR spectra. DMSO-d₆ was used as the solvent, and its residual peaks were used for calibration of the chemical shifts that are provided in parts per million (δ). Data for the elemental analyses were obtained from a Vario EU III instrument. All solvents were of analytical or synthetic grade. For all in vitro experiments, cis-diamidodichlorido platinum(II) (CDDP), cisplatin, was provided by Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany. A FractionPREP Cell Fractionation Kit from Bio Vision, Milpitas, CA USA, was used to perform the cell fractionation prior to the ICP-MS analyses. Buffer solutions were freshly prepared in deionized water.