Neb hifi master mix

Cloning and plasmid propagation were conducted in chemically competent NEBTurbo cells (New England Biolabs). Expression of recombinant peptides and proteins was performed in chemically competent BL21 Star (DE3) (Thermo Fisher Scientific). Primers and double-stranded DNA fragments were purchased from Integrated DNA Technologies. Media used for bacterial cultures were purchased from Fisher Scientific. Phusion DNA polymerase, and NEB HiFi Master Mix were purchased from New England Biolabs. Sanger sequencing of plasmids was performed by ACGT, Inc. Plasmid DNA was purified using QIAprep spin columns (Qiagen). HisTrap columns were purchased from GE Healthcare. Gels for SDS-PAGE were purchased from Bio-Rad Labs. Protein solutions were concentrated using Amicon Ultra Centrifugal filters (Sigma Millipore). Antibiotics were purchased from GoldBio. All other chemicals were purchased from Fisher Scientific unless otherwise noted. HalA2, HalM2, CylL_S, and CylL_L were expressed and purified as previously described.^{58 (link), 69 (link)}