Prism 4.02 for windows

The cytotoxic effects of the amino acid ruthenium complexes were evaluated using an MTT assay with S180 tumor cells and L929 normal cells as described previously [18] (link). Briefly, 1.0×10⁵ S180 cells and 2.0×10⁴ L929 cells were plated in 96-well tissue culture plates and treated with different concentrations of amino acid ruthenium complexes (0.2–200 µM) for 48 h. After treatment, 10 µL of MTT (5 mg mL⁻¹) was added to each well, and the plates were incubated at 37°C for an additional 3 h. The purple formazan crystals were dissolved in 50 µL of SDS, and the absorbance was determined at 545 nm using a Stat Fax 2100 microplate reader (Awareness Technology, Palm City, FL, USA). The cell viability was calculated as follows: viability (%)  =  (absorbance of the treated wells)/(absorbance of the control wells) ×100. The IC₅₀ (complex concentration (µM) that results in a 50% reduction in cellular viability) was obtained from dose-response curves using GraphPad Prism 4.02 for Windows (GraphPad Software, San Diego, CA, USA).
The IC₅₀ values obtained from the MTT assay were used as a reference for the selection of the most promising amino acid ruthenium complex for further testing.

Results were expressed as mean ± standard deviation (mean ± SD). All analyses were carried out with GraphPad Prism 4.02 for Windows (GraphPad Software, San Diego, CA). Significant differences between treatment effects were determined by one-way analysis of variance (ANOVA), followed by Tukey's test to correct for multiple comparisons with an acceptable statistical level of significance set to 0.05.

Results were expressed as mean ± standard deviation (mean ± SEM). All analyses were carried out with GraphPad Prism 4.02 for Windows (GraphPad Software, San Diego, CA). Significant differences between treatment effects were determined by one-way ANOVA, followed by Tukey’s post-hoc test for multiple comparisons with statistical significance of p < 0.05.

Statistical analysis was performed using GraphPad Prism 4.02 for Windows (GraphPad Software Inc., La Jolla, CA, USA). The statistical tests included Mann-Whitney U tests (comparing two groups differing in one parameter) or Wilcoxon matched pairs test. Permeabilization kinetics analysis was performed by non-linear regression analysis utilizing one phase exponential association.