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2 protocols using mouse ifn γ pe

1

Multiparameter Flow Cytometry for Immune Cell Analysis

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mouse CD4–PE (eBioscience, 12-0042-82), mouse NK1.1–allophycocyanin (BioLegend, 108,710), mouse CD8a–PE–cyanine 7 (eBioscience, 25-0081-82), mouse CD69–PE (Biolegend, 104508), human/mouse granzyme B–FITC (BioLegend, 515403), mouse CD314 (NKG2D)-PE-cyanine 7 (eBioscience, 25-5882-81), mouse IFN-γ–PE (eBioscience, 12-7311-81), mouse CD45-eFluor 450 (eBioscience, 48-0459-41), mouse CD107a-V450 (BD, 560648), mouse CD8a-allophycocyanin (eBioscience, 17-0081-81), mouse CD279 (PD-1)-PE (BioLegend, 135205), mouse CD19–PE–cyanine 7 (eBioscience, 25-0193-81), Ki67 (Abcam, ab15580), cleaved caspase-3 (Asp175) (CST, 9661S), CD31 (Abcam ab, 28364), FITC-labeled AffiniPure F(ab)2 fragment donkey anti-mouse IgG (H + L) (Jackson Lab, 715-096-151), and Alexa Fluor 647-labeled AffiniPure F(ab)2 fragment goat anti-rabbit IgG (H + L) (Jackson Lab, 111-606-045). Mouse monoclonal antibody (mAb) to DKK2 (5F8) was generated by standard hybridoma technology through immunization of mice with a synthetic peptide (KLNSIKSSLGGETPGC) of human DKK2 at AbMax (Beijing, China). Therapeutic antibodies to VEGFR were Clone DC101 (BioXcell, BE0060) with rat IgG1 isotype control (BioXcell, BE0091) as the control IgG.
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2

Multiparameter Flow Cytometry for Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
mouse CD4–PE (eBioscience, 12-0042-82), mouse NK1.1–allophycocyanin (BioLegend, 108,710), mouse CD8a–PE–cyanine 7 (eBioscience, 25-0081-82), mouse CD69–PE (Biolegend, 104508), human/mouse granzyme B–FITC (BioLegend, 515403), mouse CD314 (NKG2D)-PE-cyanine 7 (eBioscience, 25-5882-81), mouse IFN-γ–PE (eBioscience, 12-7311-81), mouse CD45-eFluor 450 (eBioscience, 48-0459-41), mouse CD107a-V450 (BD, 560648), mouse CD8a-allophycocyanin (eBioscience, 17-0081-81), mouse CD279 (PD-1)-PE (BioLegend, 135205), mouse CD19–PE–cyanine 7 (eBioscience, 25-0193-81), Ki67 (Abcam, ab15580), cleaved caspase-3 (Asp175) (CST, 9661S), CD31 (Abcam ab, 28364), FITC-labeled AffiniPure F(ab)2 fragment donkey anti-mouse IgG (H + L) (Jackson Lab, 715-096-151), and Alexa Fluor 647-labeled AffiniPure F(ab)2 fragment goat anti-rabbit IgG (H + L) (Jackson Lab, 111-606-045). Mouse monoclonal antibody (mAb) to DKK2 (5F8) was generated by standard hybridoma technology through immunization of mice with a synthetic peptide (KLNSIKSSLGGETPGC) of human DKK2 at AbMax (Beijing, China). Therapeutic antibodies to VEGFR were Clone DC101 (BioXcell, BE0060) with rat IgG1 isotype control (BioXcell, BE0091) as the control IgG.
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