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Lichroprep rp 18 40 63 μm

Manufactured by Merck Group
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Sourced in Spain, United States

LiChroprep® RP-18 (40–63 μm) is a silica-based reversed-phase chromatography material with particle sizes ranging from 40 to 63 micrometers. It is designed for use in liquid chromatography applications.

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Lab products found in correlation

Avance 3 500 spectrometer, by Bruker (3 mentions) L dihydroxyphenylalanine l dopa, by Merck Group (3 mentions) Kieselgel 60 f254, by Merck Group (3 mentions) Uv 1800 spectrophotometer, by Shimadzu (3 mentions) Microtof q 2 mass spectrometer, by Bruker (3 mentions) Rp 18 f254, by Merck Group (3 mentions) 6550 ifunnel q tof, by Agilent Technologies (2 mentions) Sep pak vac 35cc 10g c18 cartridge, by Waters Corporation (2 mentions) Prepstar hplc system, by Agilent Technologies (2 mentions) Agilent 1290 infinity hplc system, by Agilent Technologies (2 mentions) Agilent 6120 quadrupole low resolution, by Agilent Technologies (2 mentions) Luna c18 2 or c8 2 100 10 μm 10.0 250 mm, by Phenomenex (2 mentions) Phenyl hexyl 5 μm, by Agilent Technologies (2 mentions) Agilent 1260 infinity hplc, by Agilent Technologies (2 mentions) Ir 408 infrared spectrometer, by Shimadzu (2 mentions) Silica gel 60, by Scharlab (2 mentions) Mushroom tyrosinase, by Merck Group (1 mentions) Ch3cn, by Merck Group (1 mentions) Silica 60 f254, by Merck Group (1 mentions) L 7000, by Hitachi (1 mentions)

7 protocols using lichroprep rp 18 40 63 μm

1

Analytical HPLC-MS Metabolite Profiling

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Analytical HPLC-MS spectra of metabolites were collected using an Agilent 1260 Infinity HPLC with an Agilent 6120 Quadrupole low-resolution (LR) electrospray ionization (ESI) mass spectrometer (Agilent, Santa Clara, CA, USA). Liquid column chromatography was carried out using either LiChroprep® RP-18 (40–63 μm) (Merck Millipore, Billerica, MA, USA) or Sep-Pak® Vac 35cc (10g) C18 cartridge (Waters, Milford, MA, USA). Preparative HPLC for the isolation of metabolites was achieved on an Agilent Prepstar HPLC system with Agilent Polaris C18-A 5 μm (21.2 × 250 mm), Phenomenex Luna C18(2) or C8(2) (100Å) 10 μm (10.0 × 250 mm) (Phenomenex, Torrance, CA, USA), and Agilent Phenyl-Hexyl 5 μm (9.4 × 250 mm) columns. High-resolution mass spectra were obtained from an Agilent iFunnel 6550 QTOF (quadrupole time-of flight) MS instrument fitted with an electrospray ionization (ESI) source coupled to an Agilent 1290 Infinity HPLC system.
Park H.B., Wei Z., Oh J., Xu H., Kim C.S., Wang R., Wyche T.P., Piizzi G., Flavell R.A, & Crawford J.M. (2020). Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coli. Nature microbiology, 5(11), 1319-1329.
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2

Tyrosinase Inhibition Assay Protocol

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Optical values were measured on a Shimadzu UV-1800 spectrophotometer (Shimadzu Pte., Ltd., Singapore). NMR spectra were acquired on a Bruker Avance III 500 spectrometer (Bruker BioSpin AG, Bangkok, Thailand). Chemical shifts are expressed as δ values. HRESIMS data were acquired on Bruker micrOTOF-QII mass spectrometer (Bruker Singapore Pte., Ltd., Singapore). Column chromatography (CC) was carried out using silica gel 60, 0.06–0.2 mm (Scharlau, Barcelona, Spain) and LiChroprep RP-18, 40–63 μm (Merck KGaA, Darmstadt, Germany). Kieselgel 60 F254 or RP-18 F254 plates for thin-layer chromatography (TLC) were purchased from Merck (Merck KGaA, Darmstadt, Germany). Mushroom tyrosinase (EC 1.14.18.1; 3933 U mL−1) and l-dihydroxyphenylalanine (l-DOPA) were obtained from Sigma-Aldrich (Sigma-Aldrich Pte Ltd, Singapore). Other chemicals were of the highest grade available.
Le T.H., Dang P.H., Nguyen H.X., Do T.N., Nguyen N.T, & Nguyen M.T. (2022). Strebluses E–H, four new stilbene-like derivatives from the stems of Streblus ilicifolius. RSC Advances, 13(1), 570-574.
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3

Analytical HPLC-MS Metabolite Profiling

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Analytical HPLC-MS spectra of metabolites were collected using an Agilent 1260 Infinity HPLC with an Agilent 6120 Quadrupole low-resolution (LR) electrospray ionization (ESI) mass spectrometer (Agilent, Santa Clara, CA, USA). Liquid column chromatography was carried out using either LiChroprep® RP-18 (40–63 μm) (Merck Millipore, Billerica, MA, USA) or Sep-Pak® Vac 35cc (10g) C18 cartridge (Waters, Milford, MA, USA). Preparative HPLC for the isolation of metabolites was achieved on an Agilent Prepstar HPLC system with Agilent Polaris C18-A 5 μm (21.2 × 250 mm), Phenomenex Luna C18(2) or C8(2) (100Å) 10 μm (10.0 × 250 mm) (Phenomenex, Torrance, CA, USA), and Agilent Phenyl-Hexyl 5 μm (9.4 × 250 mm) columns. High-resolution mass spectra were obtained from an Agilent iFunnel 6550 QTOF (quadrupole time-of flight) MS instrument fitted with an electrospray ionization (ESI) source coupled to an Agilent 1290 Infinity HPLC system.
Park H.B., Wei Z., Oh J., Xu H., Kim C.S., Wang R., Wyche T.P., Piizzi G., Flavell R.A, & Crawford J.M. (2020). Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coli. Nature microbiology, 5(11), 1319-1329.
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4

Spectroscopic Analysis of Organic Compounds

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Optical rotations were performed with a JASCO P-2000 polarimeter (Tokyo, Japan). MS spectra were obtained on Bruker Daltonik Esquire 2000 (ESIMS, ESIMS/MS) and micrOTOF orthogonal ESI-TOF (HRESIMS) mass spectrometers (Bruker Daltonics, Bremen, Germany). 1D and 2D NMR spectra were recorded in CD3OD (δH 3.30 and δC 49.0) on a Bruker Avance III 600 spectrometer, equipped with a dual-cryo probe (Karlsruhe, Germany). Centrifugal partition chromatography (CPC; model LLB-M) was purchased from Sanki Engineering Co. (Kyoto, Japan). Column Chromatography (CC) was carried out on pre-packed Lobar columns (size A, 240 × 10 mm; size B, 310 × 25 mm; size C, 440 × 37 mm, LiChroprep RP-18, 40–63 μm, Merck, Darmstadt, Germany) and Sephadex LH-20 (Pharmacia Co., Stockholm, Sweden). TLC plate (aluminum sheets, Silica 60 F254, 20 × 20 cm, 0.2 mm thick, Merck) was used analytically. HPLC system consisted of a Hitachi L-7000 pump, a Hitachi L-7000 UV detector monitored at 210 nm, and a Prodigy ODS3 100A column (semi-prep., 250 × 10 mm, 5 μm, Phenomenex, California, USA), delivered by a mixture of solvent A, 0.1% formic acid (Merck) in H2O, and solvent B, 0.1% formic acid in CH3CN (Merck), with flow rate 2.3 mL/min.
Chen H.Y., Liu J.F., Tsai S.F., Lin Y.L, & Lee S.S. (2017). Cytotoxic protobassic acid saponins from the kernels of Palaquium formosanum. Journal of Food and Drug Analysis, 26(2), 557-564.
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5

Spectroscopic Analysis of Tyrosinase Inhibition

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Optical values were measured on a Shimadzu UV-1800 spectrophotometer (Shimadzu Pte., Ltd., Singapore). IR spectra were measured with a Shimadzu IR-408 infrared spectrometer (Shimadzu Pte., Ltd., Singapore). NMR spectra were acquired on a Bruker Avance III 500 spectrometer (Bruker BioSpin AG, Bangkok, Thailand). Chemical shifts are expressed as δ values. HRESIMS data were acquired on Bruker micrOTOF-QII mass spectrometer (Bruker Singapore Pte., Ltd., Singapore). Column chromatography was carried out using silica gel 60, 0.06–0.2 mm (Scharlau, Barcelona, Spain) and LiChroprep RP-18, 40−63 μm (Merck KGaA, Darmstadt, Germany). Kieselgel 60 F254 or RP-18 F254 plates for TLC were purchased from Merck (Merck KGaA, Darmstadt, Germany). Tyrosinase (EC 1.14.18.1) from mushroom (3933 U·mL−1) and L-dihydroxyphenylalanine (l-DOPA) were obtained from Sigma-Aldrich (Sigma-Aldrich Pte., Ltd., Singapore). Other chemicals were of the highest grade available.
Dang P.H., Le T.H., Do T.N., Nguyen H.X., Nguyen M.T, & Nguyen N.T. (2021). Diarylalkanoids as Potent Tyrosinase Inhibitors from the Stems of Semecarpus caudata. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 8872920.
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6

Spectroscopic Analysis of Natural Compounds

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Optical values were measured on a Shimadzu UV-1800 spectrophotometer (Shimadzu Pte., Ltd., Singapore). IR spectra were measured with a Shimadzu IR-408 infrared spectrometer (Shimadzu Pte., Ltd., Singapore). NMR spectra were acquired on a Bruker Avance III 500 spectrometer (Bruker BioSpin AG, Bangkok, Thailand). Chemical shifts are expressed as δ values. HRESIMS data were acquired on Bruker micrOTOF-QII mass spectrometer (Bruker Singapore Pte., Ltd., Singapore). Column chromatography was carried out using silica gel 60, 0.06–0.2 mm (Scharlau, Barcelona, Spain), and LiChroprep RP-18, 40−63 μm (Merck KGaA, Darmstadt, Germany). Kieselgel 60 F254 or RP-18 F254 plates for TLC were purchased from Merck (Merck KGaA, Darmstadt, Germany). Tyrosinase (EC 1.14.18.1) from mushroom (3933 U·mL−1) and l-dihydroxyphenylalanine (l-DOPA) were obtained from Sigma-Aldrich (Sigma-Aldrich Pte Ltd, Singapore). Other chemicals were of the highest grade available.
Nguyen N.T., Dang P.H., Nguyen H.X., Do T.N., Le T.H., Le T.Q, & Nguyen M.T. (2021). Tyrosinase Inhibitors from the Stems of Streblus Ilicifolius. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 5561176.
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7

Spectroscopic and Structural Analysis of Compounds

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Melting points were measured on an electrothermal apparatus. IR and VCD spectra were recorded on a BioTools ChiralIR 2× VCD spectrometer (BioTools, Inc., Jupiter, FL, USA). X-ray data were obtained on a Bruker D8 Venture Geometry diffractometer. The 1D and 2D NMR experiments were performed on a Bruker AVANCE III HD 400 MHz and a Bruker AVANCE III HD 700 MHz. Chemical shifts were referenced to tetramethylsilane, and J values are given in Hz. The HRESIMS data were acquired on a Jeol AccuTOF JMS T100LC spectrometer. Silica gel 40–63 μm (Aldrich) and LiChroprep RP-18 40–63 μm (Merck) were used for column chromatography (CC). Thin-layer chromatography (TLC) was carried out on silica gel 60 F254 (Merck).
Melchor-Martínez E.M., Silva-Mares D.A., Torres-López E., Waksman-Minsky N., Pauli G.F., Chen S.N., Niemitz M., Sánchez-Castellanos M., Toscano A., Cuevas G, & Rivas-Galindo V.M. (2017). Stereochemistry of a Second Riolozane and Other Diterpenoids from Jatropha dioica. Journal of natural products, 80(8), 2252-2262.
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