Boron dipyrromethene bodipy

Manufactured by Thermo Fisher Scientific

Sourced in United States

Boron dipyrromethene (BODIPY) is a fluorescent dye compound commonly used in various laboratory applications. It exhibits strong absorption and emission properties, making it a useful tool for fluorescence-based techniques. BODIPY dyes have a characteristic boron-containing structure that contributes to their photophysical characteristics.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Calcofluor white cfw, by Merck Group (1 mentions) Leica 3d analysis toolkit, by Leica camera (1 mentions) Tcs sp8 confocal microscope, by Leica camera (1 mentions) Matlab, by MathWorks (1 mentions)

3 protocols using boron dipyrromethene bodipy

Visualization of Fungal Cell and Organelle Structures

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The fungal cell wall and hyphal septum were visualized by staining with 20 μg/mL calcofluor white (CFW) (Sigma-Aldrich, St. Louis, MO, USA), and mycelial cell nuclei were visualized by staining with 20 μg/mL 4′,6-diamidino-2-phenylindole (DAPI) and 20 μg/mL CFW (63 (link)). As previously described, lipid droplets (LDs) were stained with 10 μg/mL boron dipyrromethene (BODIPY) (Thermo Fisher Scientific, Waltham, MA, USA) (29 (link)). In addition, autophagy of the hyphae was detected by 100 μg/mL monodansylcadaverine (MDC) staining.
For TEM analysis, WT and mutant strains were harvested from colonies cultured on PD broth for 3 days. Then, the mycelia were collected and fixed with 2.5% glutaraldehyde for TEM observation (30 (link)).

Liu Q., Li D., Bai N., Zhu Y, & Yang J. (2023). Peroxin Pex14/17 Is Required for Trap Formation, and Plays Pleiotropic Roles in Mycelial Development, Stress Response, and Secondary Metabolism in Arthrobotrys oligospora. mSphere, 8(2), e00012-23.

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Sporulation and Appressorium Development in Fungi

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For sporulation, 3 ml of double-distilled water (ddH₂O) was added to the CM plate to obtain conidia, and then quantitative statistics were performed. Appressorium development was observed on hydrophobic surfaces with 20 μl of spore suspensions (5 × 10⁴ conidia/ml) at 22°C. Conidial germination was observed at 4 h. Appressorium formation was observed at 4, 6, 8, and 24 h. Collapse of appressoria was observed at 24 h, and the glycerol solutions used were 0.25, 0.5, and 1.0 M. Lipid droplet staining was observed at 0, 8, 16, and 24 h using the fluorescent dye boron dipyrromethene (BODIPY) (Thermo Fisher Scientific, United States), and tricyclazole was used in spore suspension to inhibit the formation of melanin in the appressorium. Glycogen staining was observed at 0, 8, 16, and 24 h using KI/I₂ solution.

Sun L., Qian H., Wu M., Zhao W., Liu M., Wei Y., Zhu X., Li L., Lu J., Lin F, & Liu X. (2022). A Subunit of ESCRT-III, MoIst1, Is Involved in Fungal Development, Pathogenicity, and Autophagy in Magnaporthe oryzae. Frontiers in Plant Science, 13, 845139.

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Quantifying Vascular and Lipid Profiles

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Hydrogels were fixed in 4% formaldehyde for 2 hours at room temperature,
permeabilized using 0.5% Triton-X for 20 minutes, blocked using 10% goat serum
for 2 hours, then stained using Rhodamine labeled Griffonia (Bandeiraea)
Simplicifolia Lectin I (GS-1; Vector Labs, Burlingame, CA, 1:100),
boron-dipyrromethene (BODIPY; ThermoFisher, Waltham, MA, D3922, 1:100), and DAPI
(ThermoFisher, Waltham, MA, R37606). The distribution of both vessels and lipid
droplets of entire wells (n = 6/group) were determined using a
Leica TCS SP8 Confocal Microscope (Buffalo Grove, IL) using a rendering of
100 µm thickness/10 µm per section of the entire well. Quantification of “Well
Coverage (%)” was performed using the Leica 3D analysis toolkit with Otsu
thresholding. Quantification of lipid droplet size (average/well) and number
(#/well) was performed using ImageJ (U. S. National Institutes of Health,
Bethesda, MD) followed by a custom MATLAB (MathWorks, Natick, MA) script
(Supplementary Text).

Acosta F.M., Stojkova K., Zhang J., Garcia Huitron E.I., Jiang J.X., Rathbone C.R, & Brey E.M. (2022). Engineering Functional Vascularized Beige Adipose Tissue from Microvascular Fragments of Models of Healthy and Type II Diabetes Conditions. Journal of Tissue Engineering, 13, 20417314221109337.

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