Porous membrane

Manufactured by BD

Sourced in Germany

Porous membranes are thin, selective barriers that allow the passage of specific molecules or particles while retaining others. They are designed to facilitate controlled filtration, separation, and diffusion processes in various laboratory and industrial applications.

Automatically generated - may contain errors

Lab products found in correlation

Cm100, by Philips (2 mentions) Vega 3 scanning electron microscope, by TESCAN (1 mentions) Glutaraldehyde, by Serva Electrophoresis (1 mentions) Uranyl acetate, by Philips (1 mentions) Uranyl acetate, by Merck Group (1 mentions) Formaldehyde, by Merck Group (1 mentions) Spot imaging software, by Nikon (1 mentions) Sb203580, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

8-µm porous membranes (2 citations)

5 protocols using porous membrane

Scanning Electron Microscopy of FLO-1 Cells

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Scanning electron microscopy was carried out as previously described^{35 (link),41 (link),45 (link),46 (link)}. FLO-1 cells cultured at the A–L and L–L interfaces on porous membranes (BD Falcon) in DMEM, A-DMEM and UroM for 1, 2 or 4 wks were fixed in 2% formaldehyde (w/v) and 2% glutaraldehyde (v/v) in 0.1 M cacodylate buffer, pH 7.4 for 2 h 45 min at 4 °C. The fixation was followed by rinsing in 0.2 M cacodylate buffer. The samples were then post-fixed in 1% (w/v) osmium tetroxide for 2 h at room temperature, dehydrated in a graded series of ethanol, dried at a critical point, spattered with gold and examined at 30 kV with a Tescan Vega3 scanning electron microscope (Brno, Czech Republic).

Tratnjek L., Sibinovska N., Kralj S., Makovec D., Kristan K, & Kreft M.E. (2021). Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing. Scientific Reports, 11, 6664.

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Transmission Electron Microscopy of FLO-1 Cells

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Transmission electron microscopy was carried out as previously described^{40 (link),45 (link)}. FLO-1 cells cultured at the A–L and L–L interfaces on porous membranes (BD Falcon) for 1, 2 or 4 wks and cell culture dishes for 1 or 2 wks in DMEM, A-DMEM and UroM were fixed with 4% (w/v) formaldehyde (Sigma) and 2.5% (v/v) glutaraldehyde (Serva, Heidelberg, Germany) in 0.1 M cacodylate buffer, pH 7.4 for 2 h 45 min. The fixation was followed by overnight rinsing in 0.1 M cacodylate buffer and post-fixation in 2% (w/v) osmium tetroxide for 1 h at room temperature. Afterwards, the samples were incubated in 2% uranyl acetate (Merck, Germany) for 1 h at room temperature. The samples were then dehydrated in a graded series of ethanol and embedded in Epon (Serva). Epon semi-thin sections were stained with 1% toluidine blue and 2% borate in distilled water and observed with a Nicon Eclipse TE microscope. Ultrathin sections were contrasted with uranyl acetate and lead citrate and observed with a transmission electron microscope (Philips CM100, (Philips, Eindhoven, The Netherlands), operation voltage 80 kV, equipped with CCD camera (AMT, Danvers, MA, USA)).

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Ultrastructural Analysis of NPU Cells

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NPU cells of the XIII passage were seeded onto porous membranes (BD Falcon) at a density of 2 × 10⁵ viable cells/cm². After three weeks in culture, NPU cells were fixed with 4% (w/v) paraformaldehyde and 2.5% (v/v) glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4 for 2 h 45 min. The fixation was followed by overnight rising in 0.33% sucrose in cacodylate buffer and a post-fixation in 1% (w/v) osmium tetroxide for 1 h at 4 °C. The samples were afterwards dehydrated in a graded series of ethanol and embedded in Epon (Serva Electrophoresis, Heidelberg, Germany). Ultrathin sections were contrasted with uranyl acetate and lead citrate and examined with TEM (Philips CM100, Eindhoven, Netherlands) at 80 kV.

Jerman U.D., Kolenc M., Steyer A., Veranič P., Prijatelj M.P, & Kreft M.E. (2014). A Novel Strain of Porcine Adenovirus Detected in Urinary Bladder Urothelial Cell Culture. Viruses, 6(6), 2505-2518.

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Quantification of Cell Migration and Invasion

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For detection of migration and invasion of

α

δ

^{+}

cells, 5

\times

^{4}

cells were added onto a porous membrane (pore size, 8

μ

m; BD Biosciences) that was coated with 2 mg/mL Matrigel. After 48-hour incubation at 37

^{\circ}

C, cells were washed three times with PBS, and fixed with 4% neutral formaldehyde prior to routine hematoxylin staining for 5 min. The number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 representative fields at

\times

20 magnification. Images were acquired and analyzed using SPOT imaging software (Nikon).

Huang C., Li Y., Zhao W., Zhang A., Lu C., Wang Z, & Liu L. (2019). 21 may be a potential marker for cancer stem cell in laryngeal squamous cell carcinoma. Cancer Biomarkers, 24(1), 97-107.

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PEG-Scl2 Hydrogel Osteogenesis and p38 Inhibition

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Concomitantly with the experiment comparing PEG-Scl2-2 to PEG-Scl2-3, an additional experiment assessing the effects of p38 activity inhibition on hMSC osteogenesis within PEG-Scl2-2 hydrogels was conducted. Towards this end, PEG-Scl2-2 gels were fabricated as previously described and immersed in DMEM supplemented with 10% MSC-qualified FBS and 1% PSA at 37 °C and 5% CO₂ for 1 h. The hydrogels were then divided into two groups: 1) hydrogels exposed to 10 μM of p38 activity inhibitor SB203580 (Cell Signaling), which was first dissolved at 1 mM in dimethylsulfoxide (DMSO) prior to addition to the culture medium, and 2) control hydrogels exposed only to equivalent amounts of DMSO vehicle. The hydrogel discs were transferred to 12-well culture inserts fitted with a porous membrane (BD Biosciences), and medium changes were performed every two days. The culture medium consisted of DMEM supplemented with 10% MSC-qualified FBS, 1% PSA, and osteogenic supplements with either inhibitor supplementation or DMSO equivalent volume. After 2 weeks of culture, samples were harvested from each hydrogel group for cell density and Western blot analyses, which were conducted as previously described.

Becerra-Bayona S.M., Guiza-Arguello V.R., Russell B., Höök M, & Hahn M.S. (2018). Influence of collagen-based integrin α1 and α2 mediated signaling on human mesenchymal stem cell osteogenesis in three dimensional contexts. Journal of biomedical materials research. Part A, 106(10), 2594-2604.

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