7683b series auto injector

The GC-MS analysis of the essential oils was carried out using a GC 6850 Network gas chromatographic system, equipped with a 7683 B Series auto injector and a 5973 I inert mass selective detector (Agilent Technologies USA), using a HP-5 MS capillary column having 5% phenyl polysiloxane as a stationary phase, column length 30.0 m, i.d. 0.25 mm and film thickness 0.25 μm. One μl of sample was injected in the split mode with split ratio 30:1 at a temperature of 300°C. Helium was used as carrier gas with a constant flow rate of 1.5 ml/min. The temperature program was as follows: initial temperature 150°C, held for 1 min and then ramping at rate of 10°C/min up to 290°C, kept constant for 5 min. The temperature of transfer line was 300°C. Electron ionization mode with the ionization energy of 70 eV having mass range scanned 3–500 m/z was used for mass spectra determination. The temperature of ion source was 230°C and that of MS quadropole 150°C. The identification of components was based on comparison of their mass spectra with those of NIST mass spectra library
[37 ,38 ].

GC–MS analysis was performed using an Agilent 6890 N Network GC System equipped with an Agilent 7683B Series auto-injector, coupled to an Agilent 5975 Inert Mass Selective Detector. The operating conditions were as follows: initial oven temperature, 50°C for 5 min, then to 150°C at 4°C/min and held for 5 min, then to 250°C at 4°C/min and held for 10 min; injector and detector temperatures, 275^oC; injection volume, 0.2 μL; split ratio, 50:1. The carrier gas used was He at 1.0 mL/min. The significant MS operating parameters were: ionization voltage, 70 eV; ion source temperature 230^oC; mass range 50–600 U (Adams, 2001 ).
GC-MS was performed for the n-hexane fraction of the ethanol extract of C. aciculatus. The constituents were identified by comparison of their mass spectra with reference spectra in the computer library (NIST 05).

For GC-MS analysis, a gas chromatograph operated with 6890N network GC system with 5975C inert XL EI/CI mass selective detector was used along with a 7683 B series auto injector for split/splitless injection (Agilent Technologies, Inc., Wilmington, DE, USA). The Agilent gas chromatograph system was equipped with a capillary column, which is made of methyl siloxane, in dimensions of 12 × 200 × 0.33 µm. The carrier gas for GC separation was helium and the linear being used was 4 mm ID tap GW along with a non-stick O-ring purchased from Agilent Technologies (Wilmington, DE, USA). 96-Well human TNFα ELISA kits (model: EH3 TNFA) purchased from Thermo Scientific (Waltham, MA, USA) were stored at −24 °C freezer and thawed at room temperature before each use.