High pure ffpe rna micro kit

As previously described (Zhou et al., 2017b (link)), total RNA was isolated from 200 µl serum or exosome samples using mirVana Paris Kit (Ambion, Austin, TX, USA) under the manufacturer’s protocol instruction. We added 5 µl of synthetic C. elegans miR-39 (5nM/L, RiboBio, Guangzhou, China) to each sample after denaturing the solution (Ambion, Austin, TX, USA) for sample-to-sample normalization. We extracted total RNA from FFPE specimens via the High Pure FFPE RNA Micro Kit (Ambion Austin, TX, USA). Then, we dissolved RNA in 100 µl RNAse-free water and stored these RNA samples at −80 °C until use. Nanodrop 2000 Thermo scientific spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) was used to evaluate the total RNA’s concentration and purity.

RNA was isolated from 200μl serum using mirVana Paris Kit (Ambion, Austin, TX, USA) according to the manufacturer’s protocol. To allow for normalization of sample-to-sample variation in RNA extraction procedures, synthetic C. elegans miRNA cel-miR-39 (5 nM/L, 5μl RiboBio, Guangzhou, China) was spiked into each denatured sample after combining the serum sample with denaturing solution (Ambion, Austin, TX, USA). Total RNA was extracted from FFPE specimens using the High Pure FFPE RNA Micro Kit (Ambion, Austin, TX, USA). RNA was eluted with 100μl of RNase-free water and stored at − 80°C for further use. The ultraviolet spectrophotometer was used to evaluate the concentration and purity of the total RNA. The concentration of serum RNA ranged from 15.73 ng/μl to 48.62 ng/μl.

RNA was isolated from 200 μL serum using mirVana Paris Kit (Ambion, Austin, TX) according to the manufacturer's protocol. Total RNA was extracted from FFPE specimens using the High Pure FFPE RNA Micro Kit (Ambion), as previously described. RNA was eluted with 100 μL of RNase‐free water and stored at −80°C for further use. The ultraviolet spectrophotometer was used to evaluate the concentration and purity of the total RNA. The concentration of serum RNA ranged from 15.26 to 50.19 ng/μL.