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Wheat germ agglutinin alexa fluor 488 and 350 dyes

Manufactured by Thermo Fisher Scientific

Wheat Germ Agglutinin (WGA) Alexa Fluor 488 and 350 dyes are fluorescent conjugates used for labeling and detecting specific glycoconjugates in biological samples. The Alexa Fluor 488 dye emits green fluorescence, while the Alexa Fluor 350 dye emits blue fluorescence. These dyes can be used in a variety of applications, such as cell staining, microscopy, and flow cytometry.

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2 protocols using wheat germ agglutinin alexa fluor 488 and 350 dyes

1

Comprehensive Immune Signaling Antibody Protocols

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Puromycin was purchased from Sigma‐Aldrich. GAPDH, β‐actin, GFP, OFP, SHP2, p‐LCK, LCK, p‐AKT (Thr308), AKT, p‐ZAP70, and ZAP70 antibodies for western blot were purchased from Abmart. FGL1 antibodies were purchased from Santa Cruz Biotechnology Inc. Antibodies, including human PD‐L1 and PD‐1 were purchased from Invitrogen. Human LAG‐3 and Na+K+ATPase antibodies were purchased from Cell Signaling Technology and Santa Cruz Biotechnology Inc. respectively. Marker antibodies for exosomes, including anti‐CD9, anti‐CD63, and anti‐ALIX, were purchased from Santa Cruz Biotechnology Inc, and anti‐CD81 from System Biosciences. Wheat Germ Agglutinin (WGA) Alexa Fluor 488 and 350 dyes were purchased from Thermo Scientific. Ficoll Paque Plus used for isolating peripheral blood mononuclear cells (PBMC) cells was purchased from GE Healthcare. Staining antibodies, including CD3, CD4, CD8, CD25, and Foxp3 for FACS analysis were purchased from Biolegend Inc.
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2

Sorafenib-Induced Oxidative Stress Assay

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Sorafenib, Calcein-AM and TMRE were purchased from Med Chem Express (MCE). DCFH-DA (2'-7'-dichlorodihydrofluorescein diacetate) was purchased from Sigma. FeCl3·6H2O and NH4Cl were purchased from MACKLIN. Sulphosalicylic acid was purchased from Aladdin. Wheat germ agglutinin (WGA) Alexa Fluor 488 and 350 dyes were purchased from Thermo Scientific. GFP and OFP fluorescin antibodies for Western blotting were purchased from Transgen. COX2 antibodies for Western blotting and TFRC, Ki67 antibodies for IHC were purchased from Cell Signaling Technology.
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