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2 protocols using poly ethylene glycol

1

Biomaterials for Tissue Engineering

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Poly(vinyl alcohol) (Mw 85–124 kDa), 3,4-dihydroxy-D-phenylalanine (DOPA), and poly(D,L-lactide-co-glycolide) (PLGA) were purchased from Sigma-Aldrich (USA). Ethyl cellulose, bovine submaxillary mucin, and poly(ethylene glycol) (PEG) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (China). Lysozyme, dopamine hydrochloride, FITC, TRITC phalloidin, DAPI, Dex, RB sodium salt, Tris buffer, and agarose were purchased from Beijing Solarbio Science & Technology Co., Ltd. (China). Alexa Fluor-555-WGA was purchased from Thermo Fisher Scientific (USA). Enhanced cell counting kit-8 (CCK-8) was obtained from Saint-Bio Co., Ltd. (China). Anti-cytokeratin 5 rabbit monoclonal antibody (CK5, Abcam, Cat no. ab52635, Lot. GR3292032-7, dilution: 1:100), anti-cytokeratin13 rabbit polyclonal antibody (CK13, Servicebio, Cat no. GB11802, dilution: 1:500), and CD11b polyclonal antibody (CD 11b, Bioss, Cat no. bs-1014R, Lot. AG05216987, dilution: 1:100).
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2

Prunus mume Fruit Pulp Extraction

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Prunus mume Sieb. et Zucc. were purchased at commercial maturity in Dazhou, Sichuan province in June. The pulps were cut from the fruits at 4 °C and frozen at −78 °C until used. Polyvinylpyrrolidone (PVPP, USP), catechol, polyethylene glycol (PEG 15000), and cellulose membranes (76 × 49 mm) were purchased from Yuanye (Shanghai, China). l-Cysteine (Biological reagent, BR) was purchased from Sigma-Aldrich (USA). Tris–HCl was purchased from Amresco (USA). 4-Hexylresorcinol (BR) was purchased from Aladdin (USA). SDS-PAGE Gel Kit and Tris were purchased from Solarbio (Beijing, China). Glycine (BR) was purchased from Biofroxx (Germany). Markers were purchased from Tiangen (China). Bovine albumin serum (BSA, BR) was purchased from Regal (Shanghai, China). All other reagents were purchased from KESHI (Chengdu, China), including the brilliant blue G250 (BR). All reagents and chemicals used in this study were of analytical grade, except where indicated otherwise.
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