Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich (St. Louis, MO, USA); Astragalus polysaccharides (APS, UV ≥ 98%) were bought from Yuan-ye Biotechnology (Shanghai, China). RPMI-1640 medium, penicillin-streptomycin solution, 0.05% trypsin-digestion solution, and fetal bovine serum were obtained from Gibco (Grand Island, NY, USA). Phosphate buffer (PBS) was purchased from HyClone (South Logan, UT, USA). Rabbit anti-GAPDH, rabbit anti-iNOS, rabbit anti-Arg-1, mouse anti-IBA-1, anti-rabbit IgG HRP-linked antibody, and anti-mouse IgG HRP-linked antibody were obtained from Cell Signaling Technology (CST, Danvers, MA, USA). Goat anti-mouse IgG H + L antibody was obtained from Biotech (Beijing, China).
Mouse anti iba 1
Manufactured by Cell Signaling Technology
Sourced in United States
Mouse anti-IBA-1 is a primary antibody that recognizes the Ionized Calcium-Binding Adapter Molecule 1 (IBA-1) protein. IBA-1 is a calcium-binding protein that is specifically expressed in microglia, the resident immune cells of the central nervous system. This antibody can be used to detect and visualize microglia in tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rabbit anti inos, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Rabbit anti arg1, by Cell Signaling Technology (1 mentions)
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Rat anti brdu, by Merck Group (1 mentions)
Donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Mouse anti gfap, by Merck Group (1 mentions)
Mouse anti β3 tubulin, by Promega (1 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse anti iba 1
1
Astragalus Polysaccharides Modulate Macrophage Phenotype
2
Immunofluorescence Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were used: mouse anti-PPARα (1:200; cat. no. 20R-PR21, Fitzgerald, Acton, MA, USA), rabbit anti-cleaved caspase-3 (1:500; cat. no. 9661, Cell Signaling), mouse anti-Iba-1 (1:500; cat. no. MABN92, Merck), mouse anti-GFAP (1:500; cat. no. G3893, Sigma), rat anti-BrdU (1:2000; cat. no. OBT0030G, Accurate Chemical and Scientific, Westbury, NY, USA), and mouse anti-β3 tubulin (1:5000; Promega, Madison, WI, USA). For double immunofluorescence, sections were incubated overnight at 4°C with a cocktail of the primary antibodies and then with the correspondent secondary antibodies: donkey anti-rat IgG (H+L) labeled with Alexa Fluor® 488 (1:1000; cat. no. A21208, Molecular Probes, Invitrogen, Paisley, UK), donkey anti-mouse IgG (H+L) labeled with Alexa Fluor® 594 (1:1000; cat. no. A21203, Molecular Probes), and donkey anti-rabbit IgG labeled with Cy3 bis-NHS ester (1:300; cat. no. 711-166-152, Jackson ImmunoResearch).
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