Sealsafe plus

Manufactured by Tecniplast

Sourced in Italy, Japan

SealSafe Plus is a modular, ventilated cage system designed for laboratory animals. It provides a controlled environment to house and care for research animals. The system features a sealed, filtrated cage design to maintain air quality and minimize contamination. SealSafe Plus offers adjustable air flow and temperature controls to support the animals' health and welfare.

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Lab products found in correlation

M z extrudat, by Ssniff (3 mentions) Wistar rats, by Tecniplast (2 mentions) Lab r a2, by Japan SLC (2 mentions) C57bl 6jrj mice, by Janvier Labs (1 mentions) Narcoren, by Boehringer Ingelheim (1 mentions) Mesocricetus auratus, by Janvier Labs (1 mentions)

Close spelling variants of this product

Sealsafe Plus GM500 (7 citations) Sealsafe plus DGM™, (4 citations) Sealsafe (3 citations) Sealsafe Plus Mouse (3 citations) Sealsafe Plus system (2 citations) Sealsafe Plus GR900 (2 citations) Sealsafe Plus GM500 cages (2 citations) Sealsafe Plus GR900 green line (2 citations)

9 protocols using sealsafe plus

Wistar Rat Pathogen-Free Housing Study

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Six 7-week-old male Wistar rats (Japan SLC, Hamamatsu, Japan) were maintained under specific pathogen-free conditions in individual ventilated cages (Sealsafe Plus; Tecniplast,
Bugugiate, Italy). They were permitted free access to water and food (Lab RA-2; Nosan Corp., Yokohama, Japan). The animal facility was maintained under a 12-hr/12-hr light/dark
cycle at 23 ± 2°C and 50 ± 10% humidity. Clinical and pathological examinations in all animals confirmed that there were no signs of disorder. This animal study was approved by the
Institutional Animal Care and Use Committee (Permission no. 25-06-01) and carried out according to the Kobe University Animal Experimentation Regulations.

NAKANISHI S., MANTANI Y., HARUTA T., YOKOYAMA T, & HOSHI N. (2020). Three-dimensional analysis of neural connectivity with cells in rat ileal mucosa by serial block-face scanning electron microscopy. The Journal of Veterinary Medical Science, 82(7), 990-999.

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Comprehensive Mouse Housing and Acclimation Protocol

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Forty-eight C57BL/6JRj mice (24 eight-week-old males and 24 eight-week-old females) were purchased from a verified international breeder (Janvier Labs, Le Genest-Saint-Isle, France). All mice were raised following the recommendations of the Federation of Laboratory Animal Science Associations (FELASA). The mice were randomly housed in groups of four in type II long filter-top cages (Tecniplast, Buguggiate, Italy; SealSafe Plus, polyphenylsulfone, 36.5 cm × 21 cm × 14 cm), equipped with red transparent shelters, cocoons, and opaque 10 cm PVC tubes (tunnels) to transport the mice in an animal-friendly way. Housing followed a 12/12 light–dark cycle (200 lux from 7:00 to 19:00) in a temperature and humidity-controlled animal room (22–24 °C and 50–55%, respectively). Water and food (ssniff M-Z Extrudat, ssniff, Soest, Germany) were provided ad libitum. The mice were kept in same-sex groups of four. All animals were allowed a habituation period of one week before the experimental phase began.

Dickmann J., Gonzalez-Uarquin F., Reichel S., Pichl D., Radyushkin K., Baumgart J, & Baumgart N. (2022). Clicker Training Mice for Improved Compliance in the Catwalk Test. Animals : an Open Access Journal from MDPI, 12(24), 3545.

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Postnatal Mouse Microstructure Analysis

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This study was carried out according to the German Animal Welfare Act guidelines and the European Directive 2010/63/EU for protecting animals used for scientific purposes. Reporting was carried out following the ARRIVE guidelines [31 ]. The offspring (postnatal 5 days—P5) were sacrificed by expert personnel for scientific use according to §4 of the Animal Welfare Act, legalized and controlled by the Animal Welfare Authority of the Medical University.
Term-pregnant C57BL/6JRj mice were purchased (Janvier Labs, Le Genest-Saint-Isle, France). After arrival, the mice were housed in type II long filter-top cages (SealSafe Plus, polyphenyl sulfone, 365 mm L × 207 mm W × 140 mm H, Greenline; Tecniplast, Buguggiate, Italy). The mice were kept in a 12:12 h light/dark cycle (lights 06:00–18:00) in a temperature- and humidity-controlled animal room (22 ± 2 °C, 55 ± 5%). Food (ssniff M-Z Extrudat, ssniff, Soest, Germany) and water were supplied ad libitum.
The pup was sacrificed with sodium pentobarbital (i.p. 0.1 mL/10 g BW, Narcoren^® (16 g pentobarbital sodium 16.0 g, benzyl alcohol 3.0 g; Boehringer Ingelheim, Ingelheim, Germany)) at P5 for micro-computed tomography.

Heuser M., Gonzalez-Uarquin F., Nuber M., Brockmann M.A., Baumgart J, & Baumgart N. (2024). A 3D-Printed Dummy for Training Distal Phalanx Amputation in Mice. Animals : an Open Access Journal from MDPI, 14(8), 1253.

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Wistar Rat Specific-Pathogen-Free Experiment

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Eight male specific-pathogen-free Wistar rats aged 7 weeks and derived from different
mothers (Japan SLC Inc., Hamamatsu, Japan) were maintained under specific-pathogen-free
conditions in individual ventilated cages (Sealsafe plus; Tecniplast Japan, Tokyo, Japan)
with controlled temperature (23 ± 1°C) and humidity (50%) on a 12-hr light/dark cycle at
the Life-Science Laboratory of Kobe University. All animals were permitted free access to
water and food (Lab R-A2; Japan SLC Inc.). Clinical and pathological examinations in all
animals confirmed no sign of disorder. The experiments were approved by the Institutional
Animal Care and Use Committee (Permission number: 25-06-01) and were carried out according
to the Kobe University Animal Experimentation Regulations.

MASUDA N., MANTANI Y., YOSHITOMI C., YUASA H., NISHIDA M., ARAI M., KAWANO J., YOKOYAMA T., HOSHI N, & KITAGAWA H. (2017). Immunohistochemical study on the secretory host defense system with lysozyme and secretory phospholipase A2 throughout rat respiratory tract. The Journal of Veterinary Medical Science, 80(2), 323-332.

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Wistar Rat Model for Preclinical Studies

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Thirteen specific-pathogen-free male Wistar rats aged 7 weeks and derived from different mothers (Japan SLC Inc., Hamamatsu, Japan) were maintained under specific-pathogen-free conditions
in individual ventilated cages (Sealsafe PLUS; Tecniplast S.p.A, Buguggiate, Italy) with controlled temperature (23 ± 1°C) and humidity (50%) under a 12-hr light/dark cycle at the
Life-Science Laboratory of Kobe University. All animals were permitted free access to water and food (Lab R-A2; Japan SLC Inc.). Clinical and pathological examinations in all animals
confirmed no sign of disorder. Experiments were approved by the Institutional Animal Care and Use Committee (permission number: 25-06-01) and were carried out according to the Kobe
University Animal Experimentation Regulations.

MASUDA N., MANTANI Y., YUASA H., YOSHITOMI C., ARAI M., NISHIDA M., QI W.M., KAWANO J., YOKOYAMA T., HOSHI N, & KITAGAWA H. (2018). Immunohistochemical study on the distribution of β-defensin 1 and β-defensin 2 throughout the respiratory tract of healthy rats. The Journal of Veterinary Medical Science, 80(3), 395-404.

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Comprehensive SPF Mouse Housing Protocol

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All mice were housed in IVC systems (GM 500, SealSafe Plus, Tecniplast, Buggugiate, Italy) under SPF conditions with a maximum density of 5 adult animals per cage. All racks, cages and cage supplies were cleaned and autoclaved prior to use. The mice were kept at the following environmental conditions; 12:12-h light:dark cycle, 20–24°C, 45–65% relative humidity. The ventilation units ran at 60 air changes per hour at positive pressure (15–22 Pa). Autoclaved wood chips (SAFE Select Fine, J Rettenmaier & Söhne GmbH, Rosenberg, Germany) and nesting material (SAFE Crinklets natural, J Rettenmaier & Söhne GmbH) were used as bedding and environmental enrichment. Sterile-filtered tap water and irradiated standard rodent chow (Altromin 1314, Altromin Spezialfutter, Lage, Germany) were available ad libitum. To access the animal husbandry complete change of clothes and shoes, passing an air shower and wearing sterilized protective clothing and gloves, mouth protection and hair net was mandatory. Cage changes and sampling were performed under a HEPA-filtered cage changing station. Routine health monitoring was performed quarterly for all FELASA-listed agents [39 (link)] based on EAP-PCR [30 (link)].

Körner C., Miller M, & Brielmeier M. (2019). Detection of Murine Astrovirus and Myocoptes musculinus in individually ventilated caging systems: Investigations to expose suitable detection methods for routine hygienic monitoring. PLoS ONE, 14(8), e0221118.

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Characterization of STAT2-/- Syrian Hamsters

Cited 2 times

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Wild-type outbred specific pathogen-free Syrian hamsters (Mesocricetus auratus) were purchased from Janvier Laboratories (France). The generation⁴⁹ (link) and characterization²⁵ (link) of STAT2^−/− (gene identifier: 101830537) hamsters has been described elsewhere. STAT2^−/− hamsters were bred in-house. Hamsters (maximum n = 2) were housed in individually ventilated cages (Sealsafe Plus, Tecniplast; cage type GR900), under standard conditions of 21°C, 55% humidity, and 12 :12 h light/dark cycles. Hamsters were provided food and water ad libitum, as well as extra bedding material and wooden gnawing blocks for enrichment as previously described. This project was approved by the KU Leuven ethics committee (P015-2020), following institutional guidelines approved by the Federation of European Laboratory Animal Science Associations (FELASA). Hamsters were euthanized by intraperitoneal administration of 500 μL (hamsters) Dolethal (200 mg/mL sodium pentobarbital; Vétoquinol SA).

Li L.H., Liesenborghs L., Wang L., Lox M., Yakass M.B., Jansen S., Rosales Rosas A.L., Zhang X., Thibaut H.J., Teuwen D., Neyts J., Delang L, & Dallmeier K. (2022). Biodistribution and environmental safety of a live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate. Molecular Therapy. Methods & Clinical Development, 25, 215-224.

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Wistar Rat Housing and Experimental Conditions

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Eight 7-week-old male Wistar rats (Japan SLC, Hamamatsu, Japan) were maintained under
specific pathogen-free conditions in individual ventilated cages (Sealsafe Plus;
Tecniplast, Bugugiate, Italy). They were permitted free access to water and food (Lab
RA-2; Nosan Corp., Yokohama, Japan). The animal facility was maintained under a
12-hr/12-hr light/dark cycle at 23 ± 2°C and 50 ± 10% humidity. This animal study was
approved by the Institutional Animal Care and Use Committee (permission no. 30-05-01) and
carried out according to the Kobe University Animal Experimentation Regulations.

NAKANISHI S., MANTANI Y., OHNO N., MORISHITA R., YOKOYAMA T, & HOSHI N. (2022). Histological study on regional specificity of the mucosal nerve network in the rat large intestine. The Journal of Veterinary Medical Science, 85(2), 123-134.

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Timed Pregnancy in C57BL/6J Mice

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Primiparous timed-pregnant C57BL/6J mice (SPF, specific-pathogen-free, tested according to the FELASA, Federation for Laboratory Animal Science Associations, recommendations 29 ) were purchased on gestation day 14 from a registered international breeder (Janvier Labs, Le Genest-Saint-Isle, France), where they were kept in type II cages (330 cm 2 ) with 100 g of bedding material (spruce, Rettenmaier, Rosenberg, Germany) enriched with nest-building material (7-8 g sizzle nest per two pregnant females). A successful mating is indicated by a vaginal plug. The day the plug was detected was considered day 0.
On arrival at our facility, all dams were single housed in type II long, filter-top cages (Tecniplast, Buguggiate, Italy; SealSafe Plus, polyphenylsulfone, 365 mm L Â 207 mm W Â 140 mm H Greenline) with varying enrichment described in experimental design. The mice were maintained under a 12:12-h light/dark cycle (lights on 06:00-18:00) in a temperature-and humidity-controlled animal room (22 AE 2 C, 55 AE 5%). Food (ssniff M-Z Extrudat, ssniff, Soest, Germany) and water were supplied ad libitum.

Leidinger C.S., Thöne-Reineke C., Baumgart N, & Baumgart J. (2019). Environmental enrichment prevents pup mortality in laboratory mice. Laboratory animals, 53(1).

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