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2 protocols using protease and phosphatase phosstop inhibitor cocktails

1

Oxidative and DNA-damaging Agents Protocol

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Oxidative and DNA-damage drugs hydroxyurea (HU), methyl methanesulfonate (MMS), 4-Nitroquinoline N-oxide (4NQO), menadione, paraquat and hydrogen peroxide were purchased from Sigma-Aldrich. Zeocin was purchased from Invitrogen, and camptothecin (CPT) was a gift from Dr. Leroy Liu of the University of Medicine and Dentistry of New Jersey. Dihydrorhodamine 123 (DHR) and dihydroethidium (DHE) were purchased from Life Technology. Mouse anti-Myc (9E10) antibody (#200613) was purchased from Harlan Laboratories. Mouse anti-GST antibody (#2624), anti-mouse horseradish peroxidase (HRP)-conjugated antibody (#7076) and anti-rabbit HRP-conjugated antibody (#7074) were purchased from Cell Signaling Technology. Anti-goat HRP-conjugated antibody (A16142) and Alexa Fluor 488-conjugated goat anti-mouse antibody (A11001) were purchased from Life Technologies. Anti-Sod1 antibody (ab-16831) and anti-PGK1 antibody (ab-113687) were purchased from Abcam. Anti-Nop1 antibody (MCA28F2) was purchased from EnCor Biotechnology. Anti-TAP antibody (CAB1001) was purchased from Thermo Scientific. Protease and phosphatase (PhosSTOP) inhibitor cocktails were purchased from Roche.
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2

Oxidative and DNA-damaging Agents Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oxidative and DNA-damage drugs hydroxyurea (HU), methyl methanesulfonate (MMS), 4-Nitroquinoline N-oxide (4NQO), menadione, paraquat and hydrogen peroxide were purchased from Sigma-Aldrich. Zeocin was purchased from Invitrogen, and camptothecin (CPT) was a gift from Dr. Leroy Liu of the University of Medicine and Dentistry of New Jersey. Dihydrorhodamine 123 (DHR) and dihydroethidium (DHE) were purchased from Life Technology. Mouse anti-Myc (9E10) antibody (#200613) was purchased from Harlan Laboratories. Mouse anti-GST antibody (#2624), anti-mouse horseradish peroxidase (HRP)-conjugated antibody (#7076) and anti-rabbit HRP-conjugated antibody (#7074) were purchased from Cell Signaling Technology. Anti-goat HRP-conjugated antibody (A16142) and Alexa Fluor 488-conjugated goat anti-mouse antibody (A11001) were purchased from Life Technologies. Anti-Sod1 antibody (ab-16831) and anti-PGK1 antibody (ab-113687) were purchased from Abcam. Anti-Nop1 antibody (MCA28F2) was purchased from EnCor Biotechnology. Anti-TAP antibody (CAB1001) was purchased from Thermo Scientific. Protease and phosphatase (PhosSTOP) inhibitor cocktails were purchased from Roche.
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