Log In Sign up
The largest database of trusted experimental protocols

Serbp1 sirna 2

Manufactured by Thermo Fisher Scientific
Visit Supplier

SERBP1 siRNA #2 is a small interfering RNA (siRNA) designed to target the SERBP1 gene. siRNAs are used to induce RNA interference and knockdown the expression of specific genes. The core function of this product is to provide a tool for researchers to study the role of the SERBP1 gene in their experiments.

Automatically generated - may contain errors

Lab products found in correlation

Non targeting control sirna, by Thermo Fisher Scientific (2 mentions) Serbp1 sirna 1, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

SiRNAs (258 citations)

2 protocols using serbp1 sirna 2

1

Efficient SERBP1 Knockdown in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pre-designed siRNAs were acquired from Thermo Fisher Scientific: control non-targeting siRNA (Cat No. AM4635), SERBP1 siRNA #1 (Cat No. 4392420, ID: s25142), SERBP1 siRNA #2 (Cat No. 4392420, ID: s25143). When at approximately 60% confluency, cells were transfected with siRNA (100 pmol/ml) using Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific). Complete IMDM was added to the 500 μl OptiMEM used for RNAi approximately 4 h post-transfection. At 48 h post-transfection, cells were trypsinized and re-suspended in IMDM to minimize clumping. At 72 h post-transfection, cells were metabolically labeled and harvested.
Rollins M.G., Shasmal M., Meade N., Astar H., Shen P.S, & Walsh D. (2021). Negative charge in the RACK1 loop broadens the translational capacity of the human ribosome. Cell reports, 36(10), 109663.
+ Open protocol
+ Expand
2

Efficient SERBP1 Knockdown in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pre-designed siRNAs were acquired from Thermo Fisher Scientific: control non-targeting siRNA (Cat No. AM4635), SERBP1 siRNA #1 (Cat No. 4392420, ID: s25142), SERBP1 siRNA #2 (Cat No. 4392420, ID: s25143). When at approximately 60% confluency, cells were transfected with siRNA (100 pmol/ml) using Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific). Complete IMDM was added to the 500 μl OptiMEM used for RNAi approximately 4 h post-transfection. At 48 h post-transfection, cells were trypsinized and re-suspended in IMDM to minimize clumping. At 72 h post-transfection, cells were metabolically labeled and harvested.
Rollins M.G., Shasmal M., Meade N., Astar H., Shen P.S, & Walsh D. (2021). Negative charge in the RACK1 loop broadens the translational capacity of the human ribosome. Cell reports, 36(10), 109663.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!