An UV-2101PC UV-Vis spectrometer from Shimadzu was used to record transmittance curves of the dried hydrogels. The studied range was 200–800 nm with 0.5 nm resolution. Samples had an average thickness of 1 mm.
Uv 2101pc uv vis spectrometer
Manufactured by Shimadzu
Sourced in Japan
The UV-2101PC UV–vis spectrometer is a laboratory instrument manufactured by Shimadzu. It is designed to measure the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum.
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Lab products found in correlation
6 protocols using uv 2101pc uv vis spectrometer
1
Hydrogel Transmittance Spectroscopy
2
Colorimetric Detection of Cytochrome C Activity
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Tetraethylorthosilicate
(TEOS), CytC (from equine heart), 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid) diammonium salt (ABTS), and Coomassie Brilliant Blue G-250 were
purchased from Sigma-Aldrich. Phosphoric acid and hydrogen peroxide
(30% solution) were purchased from EMD Millipore. HRP was purchased
from Gold Biotechnology. Guaiacol was purchased from Cayman Chemical
Company. Premium grade glass coverslips (25 mm × 25 mm ×
1 mm) were purchased from Fisher Scientific. All chemicals and materials
were used as received, with the exception of the glass coverslips,
which were cleaned prior to use. All UV–vis spectra were obtained
via a Shimadzu UV-2101PC UV–vis spectrometer.
(TEOS), CytC (from equine heart), 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid) diammonium salt (ABTS), and Coomassie Brilliant Blue G-250 were
purchased from Sigma-Aldrich. Phosphoric acid and hydrogen peroxide
(30% solution) were purchased from EMD Millipore. HRP was purchased
from Gold Biotechnology. Guaiacol was purchased from Cayman Chemical
Company. Premium grade glass coverslips (25 mm × 25 mm ×
1 mm) were purchased from Fisher Scientific. All chemicals and materials
were used as received, with the exception of the glass coverslips,
which were cleaned prior to use. All UV–vis spectra were obtained
via a Shimadzu UV-2101PC UV–vis spectrometer.
3
Silica-Polyethylenimine Hybrid Coatings
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Tetraethylorthosilicate
(TEOS) and 600 and 2500 MW branched polyethylenimines (BPEIs) were
purchased from Sigma-Aldrich. The 600 MW BPEI has a primary/secondary/tertiary
amine ratio of 1:2:1, respectively, and the 25 000 MW BPEI
has a ratio of 1:1.2:0.76. Phosphoric acid was purchased from EMD
Millipore. Premium-grade glass coverslips (25 mm × 25 mm ×
170 μm) were purchased from Fisher Scientific. All chemicals
and materials were used as received, with the exception of the glass
coverslips, which were cleaned prior to use. All UV–vis spectra
were obtained via a Shimadzu UV-2101PC UV–vis spectrometer.
(TEOS) and 600 and 2500 MW branched polyethylenimines (BPEIs) were
purchased from Sigma-Aldrich. The 600 MW BPEI has a primary/secondary/tertiary
amine ratio of 1:2:1, respectively, and the 25 000 MW BPEI
has a ratio of 1:1.2:0.76. Phosphoric acid was purchased from EMD
Millipore. Premium-grade glass coverslips (25 mm × 25 mm ×
170 μm) were purchased from Fisher Scientific. All chemicals
and materials were used as received, with the exception of the glass
coverslips, which were cleaned prior to use. All UV–vis spectra
were obtained via a Shimadzu UV-2101PC UV–vis spectrometer.
4
UV Transmittance of Hydrogels
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The transmittance of the dried hydrogels was investigated using a UV-2101PC UV–VIS spectrometer (Shimadzu, Kyoto, Japan). The studied range was set to 200–800 nm with a 0.5-nm step width. Samples had an average thickness of 1 mm.
5
Absorption and Transmittance Analysis of Hydrogels
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The absorbance spectra of the THPTS and TMPyP as well as the transmittance of the dried hydrogels were investigated using an UV-2101PC UV/VIS spectrometer (Shimadzu, Kyoto, Japan). The studied range was set to 200–800 nm with 0.5 nm step width. Samples had an average thickness of 210 μm.
6
UV-VIS Spectroscopy Hydrogel Analysis
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UV/VIS spectra were recorded with an UV-2101PC UV-VIS spectrometer (Shimadzu, Kyoto, Japan) using Milli-Q water as solvent. Extinction coefficients were calculated at the maximum absorption wavelength using Beer–Lambert-law.39 (link) Eλ is defined as extinction at the wavelength λ. I0 is the initial intensity of the light, while I is the intensity of light after passing the sample. ελ is the molar extinction coefficient, which depends on the wavelength and the sample. d is the diameter of the cuvette. In this case d was 1 cm. T is the transmittance of the hydrogels. The concentrations c of the different samples are listed in Table 1 .
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