N methyl n tert butyldimethylsilyl trifluoroacetamide mtbstfa 1 tert butyldimethylchlorosilane tbdmcs

Standards of all metabolites were purchased from several vendors, such as Sigma-Aldrich (St. Louis, MO, USA) and Tokyo Chemical Industry (Kita-ku, Tokyo, Japan). HPLC-grade water, methanol (MeOH), and acetonitrile (ACN) were purchased from J.T. Baker Inc. (Phillipsburg, NJ, USA). LC-MS-grade formic acid was purchased from Thermo Scientific (Waltham, MA, USA). Spin-X centrifuge filter 0.45 μm with cellulose acetate was purchased from Costar (Corning, NY, USA). Triethylamine (TEA), hematoxylin and eosin (H&E), PPE, and LPS (Escherichia coli O111:B4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS) was purchased from Gibco (Grand Island, NY, USA). Toluene, diethyl ether (DEE), ethyl acetate (EA), dichloromethane (DCM), and sodium chloride (pesticide grade) were purchased from Kanto Chemical (Chuo-ku, Tokyo, Japan). Sodium hydroxide (NaOH) and sulfuric acid were obtained from Daejung Reagents Chemicals (Siheung, South Korea). O-methoxyamine hydrochloride (MO) and N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) + 1% tert-butyldimethylchlorosilane (TBDMCS) were obtained from Thermo Scientific (Bellefonte, PA, USA). All other chemicals were of analytical grade.