Human keratinocytes cells were treated with 5 µmoL of long chain ceramides, C18:0 and C 24:0 (Avanti) and collected 48 hours post-treatment. Nuclear protein was extracted using Nuclear Extraction Kit (RayBiotech) and PPARB trans-activity was measured using PPAR delta Transcription Factor Assay Kit (Abcam) as per manufacturer’s instructions. Details in Supplemental Information.
Nuclear extraction kit
Manufactured by RayBiotech
The Nuclear Extraction Kit is a laboratory tool designed to isolate and extract nuclear components from cells. It provides a standardized protocol for the separation and purification of nuclear fractions, which can be used for various downstream analyses and applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nuclear extraction kit
1
Ceramide-Induced PPAR Delta Activity
2
PPAR delta Activation by Ceramides
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