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Pbv luc

Manufactured by Addgene
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The PBV-Luc is a lab equipment product that serves as a reporter system. It contains a luciferase gene under the control of a promoter, enabling the measurement of promoter activity in cellular assays.

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Lab products found in correlation

Sbe4 luc, by Addgene (2 mentions) Prl tk renilla luciferase plasmid, by Promega (2 mentions) Glomax multi detection system, by Promega (2 mentions) Pcdna3.1 ccdb renilla, by Addgene (1 mentions) Renilla luciferase plasmid, by Addgene (1 mentions) Lenticas9 blast, by Addgene (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Dual glo luciferase assay system, by Promega (1 mentions)

Close spelling variants of this product

PBV-Luc vector

4 protocols using pbv luc

1

ESRP1 Promoter-Luciferase Assay

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MCF7 cells (0.05 × 106) were seeded in 24-well plates and cultured for 16 h. The cells were co-transfected with different ESRP1 promoter–luciferase constructs, and pRL-TK Renilla luciferase plasmid (Promega, E2231) harvested at 48 h and lysed in passive lysis buffer. For TGF-β and normoxic/hypoxic experiments, cells were transfected with SBE4-Luc (Addgene, 16495) or pBV-Luc (Addgene, 16539). After 12 h of transfection, respective treatments were given. The firefly luciferase activities were measured in a GloMax-Multi Detection System (Promega), and the values were normalized to Renilla luciferase activities. The relative values are represented as mean ± SD of triplicates from a representative experiment.
Ahuja N., Ashok C., Natua S., Pant D., Cherian A., Pandkar M.R., Yadav P., Narayanan S.S. V., Mishra J., Samaiya A, & Shukla S. (2020). Hypoxia-induced TGF-β–RBFOX2–ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancer. NAR Cancer, 2(3), zcaa021.
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2

ESRP1 Promoter-Luciferase Assay

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MCF7 cells (0.05 × 106) were seeded in 24-well plates and cultured for 16 h. The cells were co-transfected with different ESRP1 promoter–luciferase constructs, and pRL-TK Renilla luciferase plasmid (Promega, E2231) harvested at 48 h and lysed in passive lysis buffer. For TGF-β and normoxic/hypoxic experiments, cells were transfected with SBE4-Luc (Addgene, 16495) or pBV-Luc (Addgene, 16539). After 12 h of transfection, respective treatments were given. The firefly luciferase activities were measured in a GloMax-Multi Detection System (Promega), and the values were normalized to Renilla luciferase activities. The relative values are represented as mean ± SD of triplicates from a representative experiment.
Ahuja N., Ashok C., Natua S., Pant D., Cherian A., Pandkar M.R., Yadav P., Narayanan S.S. V., Mishra J., Samaiya A, & Shukla S. (2020). Hypoxia-induced TGF-β–RBFOX2–ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancer. NAR Cancer, 2(3), zcaa021.
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3

Dual-Luciferase Assay for β-Catenin

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Cells were cotransfected with β-catenin luciferase reporter constructs based on the backbone of luciferase plasmid (pBV-Luc, Addgene, Watertown, MA, USA) and Renilla plasmid (pcDNA3.1-ccdB-Renilla, Addgene, Watertown, MA, USA) according to the manual instruction. Cell lysis and luciferase activity were measured using the Luc-Pair™ Dual-Luminescence Assay Kit 2.0 (GeneCopoeia, Rockville, MD, USA) according to the manufacturer's instructions.
Jiao Y., Zhou J., Jin Y., Yang Y., Song M., Zhang L., Zhou J, & Zhang J. (2020). Long Non-coding RNA TDRKH-AS1 Promotes Colorectal Cancer Cell Proliferation and Invasion Through the β-Catenin Activated Wnt Signaling Pathway. Frontiers in Oncology, 10, 639.
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4

Transcriptional Regulation via YAP1-TEAD2 Axis

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A 500 bp region spanning the Jdp2 or control enhancers (Supplementary Information) were cloned from Hepa1-6 genomic DNA into a firefly luciferase reporter (pBV-luc; Addgene #16539), followed by the SV40 promoter. TEAD2 plasmid was generated by subcloning the TEAD2 sequence from Addgene #33107 into lentiCas9-blast (Addgene #52962) to replace the Cas9 sequence. 293fs cells were plated (6-well) and transfected with 800 ng of YAP1 plasmid (Addgene #86497), 800 ng of TEAD2 plasmid, 400 ng of firefly luciferase reporter plasmid, and 400 ng of Renilla luciferase plasmid (Addgene #27163) using lipofectamine 3000 (Invitrogen) for 72 hours. Luciferase activity was measured using the Dual-glo luciferase assay system (Promega) according to the manufacturer's protocol. Finally, we normalized firefly luciferase activity to Renilla.
Rodríguez T.C., Kwan S.Y., Smith J.L., Dadafarin S., Wu C.H., Sontheimer E.J, & Xue W. (2023). Multiomics characterization of mouse hepatoblastoma identifies yes-associated protein 1 target genes. Hepatology (Baltimore, Md.), 78(1).
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