4 dinitrobenzene

Manufactured by Merck Group

Sourced in United States

4-dinitrobenzene is a chemical compound used in various laboratory applications. It serves as a precursor for the synthesis of other chemical substances and is employed in analytical procedures. The core function of 4-dinitrobenzene is to facilitate chemical reactions and analyses in controlled laboratory environments.

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Lab products found in correlation

Close spelling variants of this product

1-chloro-2,4-dinitrobenzene (CDNB) 1-fluoro-2,4-dinitrobenzene (DNFB) 1,4-dinitrobenzene (DNB); 1-Chloro-2,4,-dinitrobenzene (CDBN) 1-chloro-2,4-dinitrobenzene 1-chloro-2,4-dinitrobenzene (CDNB), 5,5-dithiobis-2-nitrobenzoic acid (DTNB), epinephrine Glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB), 5′,5′-dithiobis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), epinephrine 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB), trichloroacetic acid (TCA), thiobarbituric acid (TBA), 1chloro-2,4-dinitrobenzene (CDNB), 1-Fluoro-2,4-dinitrobenzene 1-2'-4' dinitrobenzene

8 protocols using 4 dinitrobenzene

Enzymatic Assays for Biochemical Analysis

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Dimethyl sulfoxide (DMSO), 1-Chloro-2, 4-Dinitrobenzene (CDNB), sodium bicarbonate, and Hydroxyl ethyl piperazineethanesulfonic acid, trypsin (bovine pancreatic trypsin), Nα-benzoyl-DL-arginine-p-nitroanilide (BAPNA), and diethylaminoethyl cellulose were purchased from Sigma-Aldrich (USA). Trichloroacetic acid (TCA), sodium pyrophosphate, phenazine methosulfate, nitroblue tetrazolium, NADH, sulfanilamide, N-1-napthylethylenediamine dihydrochloride (NEDD), orthophosphoric acid, dithiobis-2-dinitrobenzoic acid (DTNB), and ethylenediaminetetraacetic acid were procured from SRL, India. All other reagents and chemicals used were of analytical grade.

Shamsi T.N., Parveen R, & Fatima S. (2017). Trypsin inhibitors demonstrate antioxidant activities, inhibit A549 cell proliferation, and increase activities of reactive oxygen species scavenging enzymes. Indian Journal of Pharmacology, 49(2), 155-160.

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Thioacetamide-Based Antioxidant Assay

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Thioacetamide (TAA), thiobarbituric acid (TBA), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 5,5-dithiobis-2-nitrobenzoic acid (DTNB), nicotinamide adenine dinucleotide hydrogen (NADH), phenazine methosulphate, trichloroacetic acid (TCA), 1-chloro-2, 4-dinitrobenzene (CDNB), nicotinamide adenine dinucleotide hydrogen phosphate (NADPH) were obtained from Sigma-Aldrich Co., St. Louis, MO, USA, and Merck (Darmstadt, Germany). All chemicals and reagents used in the experiments were of analytical grade (AR).

Ganesan K., Sukalingam K, & Xu B. (2017). Solanum trilobatum L. Ameliorate Thioacetamide-Induced Oxidative Stress and Hepatic Damage in Albino Rats. Antioxidants, 6(3), 68.

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Comprehensive Enzymatic Assay Protocol

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Acetaminophen, bufurolol, 1’-hydroxybufurolol, caffeine, dexamethasone, ethoxyresorufin, glutathione (reduced), mephenytoin, 4-hydroxymephenytoin, pentoxyresorufin, resorufin, phenacetin, warfarin, 7-hydroxywarfarin, phenylmethanesulfonyl fluoride, p-nitrophenol (PNP), UDP-glucuronic acid (ammonium salt) (UDPGA), 1-chloro-2, 4-dinitrobenzene (CDNB), 2-mercaptoethanol, 3’-Phosphoadenosine-5’-phosphosulfate (PAPS), flavin adenine dinucleotide, dicoumarol, 2,6 dichlorophenolindophenol, 2-naphthylsulfate, high performance liquid chromatography (HPLC) grade acetonitrile, and methanol were purchased from Sigma–Aldrich (St. Louis, MI, USA). Nicotinamide adenine dinucleotide phosphate (NADPH) and dimethyl sulfoxide were purchased from SRL Pvt. Ltd (Mumbai, Maharashtra, India). Testosterone, 6b-hydroxyTestosterone, chlorzoxazone, and 6-hydroxychlorzoxazone were purchased from Cayman chemical company (USA). Ultrapure water (18.2 M/Ω cm) was obtained from Milli-Q PLUS PF water. All other chemicals were commercially available or HPLC grade.

Kumar D., Trivedi N, & Dixit R.K. (2016). Evaluation of the synergistic effect of Allium sativum, Eugenia jambolana, Momordica charantia, Ocimum sanctum, and Psidium guajava on hepatic and intestinal drug metabolizing enzymes in rats. Journal of Intercultural Ethnopharmacology, 5(4), 372-382.

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Comparative Evaluation of ED Drug Profiles

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Sildenafil, tadalafil, vardenafil pills manufactured by Pfizer Pharmaceutical Company, Lilly Corporation, Schering-Plough Corporation, USA, respectively, were obtained from local drug stores at Egypt. Sulfosalcylic acid, bis-(3-caboxy-4-nitrophenyl)-disulfide, 1-chloro-2, 4-dinitrobenzene and all other chemicals were purchased from Sigma Chemical Co., St. Louis, MO, USA. Monoclonal antibodies of glutathione S-transferase π and glutathione peroxidase were obtained from ABCAM, UK.

Sheweita S., Salama B, & Hassan M. (2015). Erectile dysfunction drugs and oxidative stress in the liver of male rats. Toxicology Reports, 2, 933-938.

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Antioxidant Enzyme Activity Assay

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All of the chemicals (ethanol, thiobarbituric acid, trichloroacetic acid, pyrogallol, triton-X-100, H2O2, 1-chloro 2, 4-dinitrobenzene and nicotinamide adenine dinucleotide phosphate) used in this study to determine the antioxidant enzyme activities, MDA level, and AChE activity were obtained from Sigma-Aldrich (Germany).

Baş H., & ERSOY D.E. (2020). Fumigant toxicity of essential oil of Hypericum perforatum L., 1753 (Malpighiales: Hypericaceae) to Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae). Turkish Journal of Entomology.

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Citrullus colocynthis Seed Oil Extraction

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Citrullus colocynthis seeds were purchased from Ede market, Osun state, Nigeria. They were ground into powder. Oil was extracted from the ground seed in a soxhlet extractor for 10 h using n-hexane [10] . Ethylacetate, n-hexane, sodium sulphate, potassium hydroxide, sulphuric acid, methanol, potassium chloride, 2,2-diphenyl-1-picrylhydrazyl, 1-chloro-2, 4-di nitrobenzene (CDNB), sodium chloride, thiobarbituric acid (TBA), epinephrine, 5′,5′-Dithiobis-2-nitrobenzoic acid (DTNB), and dimethylsulphoxide (DMSO) were purchased from Sigma Chemical Company (USA). All other chemicals used were of analytical grade and were obtained from Sigma-Aldrich, St. Louis, USA.

Adewuyi A., Otuechere C.A., Ellah N.H., Kaki S.S., Fayemi S., & Adeosun C.B. (2021). Evaluation of the hepato-renal functions and antimicrobial activity of fatty amido benzoic acid synthesised from Citrullus colocynthis seed oil. Applied Biological Chemistry, 64(1).

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Evaluation of Hepatotoxicity Markers

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Poly (lactide-co-glycolide), arsenic trioxide, thiobarbituric acid, 5'-5' dithiobis-2-nitrobenzoic acid, 1-chloro 2, 4-dinitrobenzene and bovine serum albumin were procured from Sigma Chemical Company (USA).
Commercial kits for the determination of alanine transaminases, aspartate transaminase, and alkaline phosphatase were procured from Span Diagnostics, Surat (India). Other chemicals and reagents used in this study were of analytical grade and procured from E. Merck (India) and Glaxo (India).

Verma Y., Rana K., Rani V., & Sv R. (2021). Hepatotoxic Manifestations of Arsenic Trioxide Loaded Poly (Lactide-co-Glycolide) Nanoparticles in Wistar Rat.

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Synthesis and Characterization of PLGA-Arsenic Trioxide

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Poly (lactide-co-glycolide), arsenic trioxide, thiobarbituric acid, 5'-5' dithiobis-2-nitrobenzoic acid, 1-chloro 2, 4-dinitrobenzene and bovine serum albumin were procured from Sigma Chemical Company (USA). Commercial kits for the determination of alanine transaminases, aspartate transaminase, and alkaline phosphatase were procured from Span Diagnostics, Surat (India). Other chemicals and reagents used in this study were of analytical grade and procured from E. Merck (India) and Glaxo (India).

Verma Y., Rana K., Rani V., & Sv R. (2021). Hepatotoxic manifestations of arsenic trioxide loaded poly (lactide-co-glycolide) nanoparticles in wistar rat.

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