Tumors from NSG and humanized mice were harvested at experimental endpoint, which were formalin-fixed (Sigma, Saint Louis, MO, USA) at room temperature for 24 h. For H&E staining, the fixed tissues were paraffin-embedded (Leica, Germany), sliced into 5 µm sections (Leica) and stained with hematoxylin and eosin (Thermo Fisher Scientific, Waltham, MA, USA). The EBER ISH was conducted for the detection of EBV-positive cells, using Leica Bond-Max autostainer as previously described [68 (link)]. For the IHC staining, the tumor sections were stained for LMP1 and LMP2A using their respective antibodies purchased from Abcam (Cambridge, MA, USA), followed by hematoxylin counterstain. All the stained images were captured using a ZEN fluorescence microscope (Zeiss, Germany) with ZEN 2 acquisition software (Zen Blue Version, Zeiss, Germany).
Zen fluorescence microscope
Manufactured by Zeiss
Sourced in Germany
The ZEN fluorescence microscope is a versatile imaging tool designed for high-performance fluorescence microscopy. It features advanced optics and a user-friendly software interface to capture and analyze fluorescence-labeled samples with precision.
Automatically generated - may contain errors
2 protocols using zen fluorescence microscope
1
Histological Analysis of Tumor Samples
2
Immunohistochemical Analysis of Tumor Samples
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Tumors were formalin-fixed (Sigma-Aldrich) at room temperature for 24 hours, paraffin-embedded (Leica, Germany), sliced into 5-μm sections, and stained for human PD-L1, human CD3, and human NCR1 using their respective antibodies purchased from Abcam (Cambridge, MA, USA), followed by hematoxylin counterstain (Thermo Fisher Scientific). The stained images were captured using a ZEN fluorescence microscope (Zeiss, Germany) with ZEN 2 acquisition software (Zen Blue Version, Zeiss).
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