Columbia blood agar base cba

The C. jejuni strains H01 and 81-176 were isolated from human patients suffering from campylobacteriosis. The strain H01 was previously isolated by our research group [10 (link)]. The strain 81-176 carries the plasmids pVir and pTet, conferring the pTet plasmid resistance to tetracycline [9 (link)]. The C. jejuni A3S strain is nalidixic acid-resistant, streptomycin-resistant, and tetracycline-susceptible [7 (link)], and it was used as the recipient strain during the mating experiments. All C. jejuni strains were grown on Columbia blood agar base (CBA, Oxoid) supplemented with 5% of defibrinated sheep blood (Oxoid, Hampshire, UK). When required, culture media was supplemented with tetracycline (TET), kanamycin (K), gentamicin (G), nalidixic acid (Nal), and streptomycin (S) at 20, 25, 10, 50, and 15 µg/mL, respectively. CBA plates were incubated for 48 h at either 37 or 42 °C under microaerophilic conditions using a CampyGen^TM atmosphere generation system (Oxoid, Hampshire, UK).

The C. jejuni strains used in this study were strain M1, associated with transmission from poultry to a human, strain 1336, isolated from a wild bird and strain 414, isolated from a bank vole [30 (link), 38 (link)]. Campylobacter strains were stored on storage beads in glycerol broth (M Lab) at -80°C. C. jejuni strains were grown on sterile Columbia Blood Agar Base (CBA, Oxoid) with 5% (v/v) defibrinated horse blood (Oxoid) at 37°C for 48 h under microaerobic conditions (85% [v/v] N₂, 5% [v/v] O₂, and 10% [v/v] CO₂), in a Whitley VA500 Workstation incubator (Don Whitley Scientific Ltd).

L. crispatus CCUG 44128, L. jensenii CCUG 44492, L. gasseri CCUG 44075, and L. iners CCUG 38955 A were grown on Columbia blood agar base (CBA) (Oxoid, Basingstoke, UK) supplemented with 5% (v/v) defibrinated horse blood (Oxoid), at 37 °C under 10% CO₂. Escherichia coli ATCC 25922 was also grown on CBA supplemented with 5% (v/v) defibrinated horse blood, but in an aerobic atmosphere at 37 °C. Candida albicans SC 5413 was grown on Sabouraud dextrose agar (SDA; Liofilchem, Waltham, MA, USA) plates and incubated for 24 h at 37 °C, as described by Fernandes et al. [47 (link)].