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Fast green to stain fibrous tissue

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Fast green is a synthetic dye used to stain fibrous tissue in laboratory settings. It is a coloring agent that can be applied to biological samples to enhance visualization and analysis under a microscope.

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Lab products found in correlation

Safranin o, by Merck Group (2 mentions) Haematoxylin staining, by Merck Group (2 mentions) Resazurin assay, by Merck Group (2 mentions) Quant it picogreen dsdna kit, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Fast Green (421 citations) Fast green stain (5 citations)

2 protocols using fast green to stain fibrous tissue

1

Evaluating Chondrocyte Matrix Formation

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During culture, metabolic activity was measured using a resazurin assay (Sigma Aldrich) at day 1,7,14, 28. After 28 days, matrix formation was quantified by biochemical assessment of GAG (dimethylmethylene Blue (DMMB),SigmaAldrich) per DNA (Quant-iT-Picogreen-dsDNA-kit, Invitrogen) according to manufacture protocols. Additionally, samples were embedded in MMA, polymerized, and saw, or paraffin embedded and cut, to visualize the cell distribution (haematoxylin staining (Sigma Aldrich)) and matrix distribution, respectively. Matrix distribution was visualized with a safranin O (Sigma Aldrich), combined with fast green to stain fibrous tissue (Sigma Aldrich). Immunohistochemistry was performed to visualize type II collagen (II-II6B3, DSHB).
Peiffer Q.C., de Ruijter M., van Duijn J., Crottet D., Dominic E., Malda J, & Castilho M. (2020). Melt electrowriting onto anatomically relevant biodegradable substrates: Resurfacing a diarthrodial joint. Materials & design, 195, 109025.
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2

Evaluating Chondrocyte Matrix Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols
During culture, metabolic activity was measured using a resazurin assay (Sigma Aldrich) at day 1,7,14, 28. After 28 days, matrix formation was quantified by biochemical assessment of GAG (dimethylmethylene Blue (DMMB),SigmaAldrich) per DNA (Quant-iT-Picogreen-dsDNA-kit, Invitrogen) according to manufacture protocols. Additionally, samples were embedded in MMA, polymerized, and saw, or paraffin embedded and cut, to visualize the cell distribution (haematoxylin staining (Sigma Aldrich)) and matrix distribution, respectively. Matrix distribution was visualized with a safranin O (Sigma Aldrich), combined with fast green to stain fibrous tissue (Sigma Aldrich). Immunohistochemistry was performed to visualize type II collagen (II-II6B3, DSHB).
Peiffer Q.C., de Ruijter M., van Duijn J., Crottet D., Dominic E., Malda J, & Castilho M. (2020). Melt electrowriting onto anatomically relevant biodegradable substrates: Resurfacing a diarthrodial joint. Materials & design, 195, 109025.
+ Open protocol
+ Expand

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