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Mcdb107 medium

Manufactured by Funakoshi
Sourced in Japan

MCDB107 medium is a nutrient-rich liquid media formulation designed to support the growth and maintenance of a variety of cell cultures. The core function of this product is to provide the necessary nutrients, growth factors, and other essential components required for optimal cell proliferation and survival in a laboratory setting.

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2 protocols using mcdb107 medium

1

HUVEC and SKOV3 Cell Xenograft

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Human umbilical vein endothelial cells (HUVECs) were purchased from PromoCell (Heidelberg, Germany). The HUVECs were maintained in MCDB107 medium (Funakoshi, Tokyo, Japan), supplemented with 10% FBS, 20 ng/mL bovine brain extract (Kyokuto Pharmaceutical Industrial Co., Ltd., Tokyo, Japan), 10 ng/mL epidermal growth factor (EGF) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), and 50 μg/mL heparin (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan). All experiments were performed using HUVECs between passages 5 and 9. The HUVEC culture dishes were coated with type I collagen (Nitta Gelatin Inc., Osaka, Japan). SKOV3 cells were purchased from ATCC (Manassas, VA) and were maintained in McCoy’s 5 A medium containing 10% FBS. All cells were maintained at 37 °C in a humidified 5% CO2/95% air atmosphere.
Female KCN nu/nu mice (5–6 weeks old) were purchased from Japan SLC Inc. (Shizuoka, Japan). SKOV3 cells (5 × 106 cells/mouse) were subcutaneously inoculated in the flanks of the mice. In vivo US imaging studies were performed when the tumors reached 100 mm3. Tumor volumes were calculated following the equation: volume (mm3) = (width × width) × (length) × 0.5. The animal use protocol and relevant experimental procedures were approved by the Committee of Animal Use and Welfare of Tokyo University of Pharmacy and Life Sciences (authorization number: P18-66).
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2

Cell Culture Protocols for Various Cell Lines

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HEK293, BT474, and SKBR3 cells were purchased from ATCC, Manassas, VA, and MCF7 cells from Health Science Research Resources Bank, Osaka, Japan. Human dermal fibroblasts (HDFs) were purchased from Cell Applications, San Diego, CA. HEK293, BT474, and MCF7 cells and HDFs were maintained in DMEM containing 10% FBS, while SKBR3 cells were maintained in McCoy’s 5 A medium containing 10% FBS. Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza, Basel, Switzerland, and maintained in MCDB107 medium (Funakoshi, Tokyo, Japan) supplemented with 10% FBS, 20 ng/mL bovine brain extract (Funakoshi), 10 ng/mL EGF (BD Biosciences, Bedford, MA), and 50 μg/mL heparin (Wako, Osaka, Japan). For HUVECs, the culture dishes were coated with 0.3 mg/mL type I collagen (Koken, Tokyo) at 37 °C for 1 h. All cells were maintained at 37 °C in a humidified 5% CO2/95% air atmosphere.
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