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Recombinant peptide n glycosidase f

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Recombinant Peptide-N-Glycosidase F is an enzyme used in the laboratory setting. Its core function is to cleave the bond between asparagine residues and carbohydrate moieties in glycoproteins.

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Lab products found in correlation

Hplc grade acetonitrile acn, by Biosolve (2 mentions) Disodium hydrogen phosphate dihydrate, by Merck Group (2 mentions) Trifluoroacetic acid tfa, by Merck Group (2 mentions) Ammonium acetate, by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Urea, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Ammonium bicarbonate, by Merck Group (1 mentions) Potassium dihydrogen phosphate, by Merck Group (1 mentions) Chloroacetamide, by Merck Group (1 mentions) Cesium iodide, by Merck Group (1 mentions) Tris 2 carboxyethyl phosphine hydrochloride, by Merck Group (1 mentions) 2 picoline borane, by Merck Group (1 mentions) 2 aminobenzoic acid 2 aa, by Merck Group (1 mentions) Super dhb, by Merck Group (1 mentions) Nonidet p40 substitute, by Merck Group (1 mentions) Analytical grade ethanol, by Merck Group (1 mentions)

Close spelling variants of this product

Recombinant peptide-N-glycosidase F (PNGase F) Peptide-N-glycosidase F N-glycosidase F

2 protocols using recombinant peptide n glycosidase f

1

Glycoprotein Sample Preparation Protocol

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Ammonium formate was purchased from Fluka
(Steinheim, Germany). Acetic acid, 2-aminobenzoic acid (2-AA), 2-picoline
borane (2-PB), ammonium acetate, ammonium bicarbonate (ABC), bovine
serum albumin (BSA), cesium iodide, cytochrome C (cyt C), dimethyl
sulfoxide (DMSO), disodium hydrogen phosphate dihydrate, Tris (2-carboxyethyl)phosphine
hydrochloride (TCEP), chloroacetamide (CAA), dithiothreitol (DTT),
iodoacetamide (IAA), nonidet P-40 substitute (NP-40), potassium dihydrogen
phosphate, sodium chloride, super-DHB, and urea were obtained from
Sigma-Aldrich (Steinheim, Germany). Formic acid (FA) and 2-propanol
(IPA) were supplied by Riedel-De Haen (Seelze, Germany). The HPLC-grade
acetonitrile (ACN) was purchased from Biosolve (Valkenswaard, The
Netherlands). TrifluoroAcetic acid (TFA) was obtained from Merck (Darmstadt,
Germany). Recombinant Peptide-N-Glycosidase F (PNGase F) was purchased
from Roche Diagnostics or Promega. Milli-Q water was provided by Purelab
ultra (ELGA Labwater, Ede, The Netherlands). EndoPro purified fromAspergillus nigerwas provided by DSM formulated in
50% glycerol.
van Schaick G., Domínguez-Vega E., Gstöttner C., van den Berg-Verleg J.H., Schouten O., Akeroyd M., Olsthoorn M.M., Wuhrer M., Heck A.J., Abello N, & Franc V. (2021). Native Structural and Functional Proteoform Characterization of the Prolyl-Alanyl-Specific Endoprotease EndoPro from Aspergillus niger. Journal of Proteome Research, 20(10), 4875-4885.
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2

Peptide-N-Glycosidase F Protocol

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Nonidet P-40 substitute (NP-40), potassium dihydrogenphosphate (KH2PO4), disodium hydrogen phosphate dihydrate (Na2HPO4 × 2 H2O), sodium chloride (NaCl), 50 % sodium hydroxide (NaOH), 1-hydroxybenzotriazole 97 % (HOBt) and super-DHB (9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid, sDHB) were obtained from Sigma-Aldrich (Steinheim, Germany). Potassium hydroxide (KOH), sodium dodecyl sulfate (SDS), analytical grade ethanol and trifluoroacetic acid (TFA) were purchased from Merck (Darmstadt, Germany). HPLC-grade acetonitrile (ACN) was purchased from Biosolve (Valkenswaard, The Netherlands) and 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC) hydrochloride was obtained from Fluorochem (Hadfield, UK). Recombinant peptide-N-glycosidase F (PNGase F) was purchased from Roche Diagnostics (Mannheim, Germany). From a Millipore Q-Gard 2 system (Amsterdam, The Netherlands) maintained at ≥ 18 MΩ milli-Q water (MQ) was generated.
Vreeker G.C., Vangangelt K.M., Bladergroen M.R., Nicolardi S., Mesker W.E., Wuhrer M., van der Burgt Y.E, & Tollenaar R.A. (2021). Serum N-glycan profiles differ for various breast cancer subtypes. Glycoconjugate Journal, 38(3), 387-395.
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