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2 protocols using glp 1 7 36

1

GLP-1 and Liraglutide Phosphate Dissolution

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On the day of the experiment, GLP-1 (7–36) (Sigma, St. Louis, MO, USA) or liraglutide (Peptides International Inc., Louisville, KY, USA) was freshly dissolved in 0.1 M phosphate-buffered saline (PBS), adjusted to pH 7.0.
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2

β-Adrenoceptor Activation Assay

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Isoproterenol, forskolin, ICI 118-551 (ICI), epinephrine, norepinephrine, salbutamol, salmeterol, ritodrine, fenoterol, alprenolol, glucagon, oxyntomodulin, GLP-1-(7-36), rolipram, zaprinast, caffeine, HEPES, IBMX, magnesium and calcium chloride, bovine serum albumin (BSA), and Whatman glass microfiber filters (grade GF/B) were purchased from Sigma-Aldrich (Broendby, Denmark). Dulbecco's modified Eagle's medium (DMEM), Hank's balanced salt solution (HBSS), fetal bovine serum (FBS), zeocin, geneticin G418, and penicillin/streptomycin were purchased from Invitrogen (Naerum, Denmark). Black 384-well plates were purchased from Greiner Bio-One (Kremsmünster, Austria). [ 3 H] dihydroalprenolol and EnVision Xcite multilabel plate reader were from PerkinElmer (Waltham, MA).
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