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Alexa546 conjugated anti mouse igg ab

Manufactured by Thermo Fisher Scientific
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The Alexa546-conjugated anti-mouse IgG Ab is a secondary antibody conjugated with the Alexa Fluor 546 fluorescent dye. It is designed to bind to mouse immunoglobulin G (IgG) antibodies, enabling their detection and visualization in various immunoassays and imaging applications.

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Lab products found in correlation

Can get signal immunoreaction enhancer solution, by Toyobo (2 mentions) Mouse anti irf4 ab, by Thermo Fisher Scientific (2 mentions) Goat anti irf4 ab, by Santa Cruz Biotechnology (2 mentions) Rabbit anti rick ab, by Abcam (2 mentions) Rabbit anti myd88 ab, by Abcam (2 mentions) Rabbit anti traf6 ab, by Abcam (2 mentions) Duolink in situ kit, by Olink (2 mentions)

Close spelling variants of this product

Alexa546-conjugated anti-mouse IgG Anti-mouse-Alexa546 Mouse anti-IgG

2 protocols using alexa546 conjugated anti mouse igg ab

1

Visualizing IRF4 Interactions in Colon Samples

Cited 1 time
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Colon tissues were harvested and fixed in 10% formalin. Deparaffinized colon sections were then obtained and incubated with mouse anti-IRF4 Ab (Invitrogen) followed by Alexa546-conjugated anti-mouse IgG Ab (Invitrogen) by using Can Get Signal immunoreaction enhancer solution (Toyobo) as described previously 43 (link). For visualization of interaction between IRF4 and RICK, MyD88, TRAF6 in colon samples, a Duolink In Situ kit was used (Olink Bioscience). Goat anti-IRF4 Ab (Santa Cruz), rabbit anti-RICK Ab, rabbit anti-MyD88 Ab, and rabbit anti-TRAF6 Ab (Abcam) were used to visualize target protein interactions. For visualization of interaction between IRF4 and RICK, MyD88, TRAF6 in human DCs, cells were fixed in 4% paraformaldehyde and subjected to the protocols suggested by the Duolink In Situ kit.
Watanabe T., Asano N., Meng G., Yamashita K., Arai Y., Sakurai T., Kudo M., Fuss I.J., Kitani A., Shimosegawa T., Chiba T, & Strober W. (2014). NOD2 Down-Regulates Colonic Inflammation by IRF4-Mediated Inhibition of K63-Linked Polyubiquitination of RICK and TRAF6. Mucosal immunology, 7(6), 1312-1325.
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2

Visualizing IRF4 Interactions in Colon Samples

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Colon tissues were harvested and fixed in 10% formalin. Deparaffinized colon sections were then obtained and incubated with mouse anti-IRF4 Ab (Invitrogen) followed by Alexa546-conjugated anti-mouse IgG Ab (Invitrogen) by using Can Get Signal immunoreaction enhancer solution (Toyobo) as described previously 43 (link). For visualization of interaction between IRF4 and RICK, MyD88, TRAF6 in colon samples, a Duolink In Situ kit was used (Olink Bioscience). Goat anti-IRF4 Ab (Santa Cruz), rabbit anti-RICK Ab, rabbit anti-MyD88 Ab, and rabbit anti-TRAF6 Ab (Abcam) were used to visualize target protein interactions. For visualization of interaction between IRF4 and RICK, MyD88, TRAF6 in human DCs, cells were fixed in 4% paraformaldehyde and subjected to the protocols suggested by the Duolink In Situ kit.
Watanabe T., Asano N., Meng G., Yamashita K., Arai Y., Sakurai T., Kudo M., Fuss I.J., Kitani A., Shimosegawa T., Chiba T, & Strober W. (2014). NOD2 Down-Regulates Colonic Inflammation by IRF4-Mediated Inhibition of K63-Linked Polyubiquitination of RICK and TRAF6. Mucosal immunology, 7(6), 1312-1325.
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