For immunofluorescent stains, we rinsed the cells and 3D cultures twice with PBS (phosphate buffered saline). Cells were then fixed through a RT 15–30 min incubation in fresh 4% paraformaldehyde aqueous solution (157–4, ElectronMicroscopy Sciences) followed by rinsing twice with PBS. Cells were permeabilized through incubation in 0.1% Triton X-100 in PBST (phosphate buffered saline with 0.1% tween®20) for 15 minutes at RT. Cell on-specific binding was blocked through overnight incubation in 3% human serum albumin in PBST at 4 °C. After a 24-hours incubation with the primary antibody solutions at 4°C, the cells were washed five times. The following antibodies (and dilutions) were used: anti-p-tau antibody (1:40, AT8, Thermo Scientific, MN1020); anti-PHF (1:1000, A gift from P. Davies, Albert Einstein College of Medicine); anti-GFAP antibody (1:500, Neuromap, N206A/8), anti-MAP2 antibody (1:200, Cell Signaling Technology, 4542); anti-CD68 (1:100, Cell Signaling, 76437); anti-cd11b (1:100, Life Technologies, NB110–89474); anti-S100 (1:400, Abcam, ab868); anti-S100A6 (1:200, Cell Signaling, D3H3W); anti-S100B (1:500, Abcam, Ab218515); anti-ALDH1L1 (1:200, EMD Millipore, MABN495).
Anti s100a6
Manufactured by Cell Signaling Technology
Anti-S100A6 is a purified antibody product. It binds to the S100A6 protein, which is a member of the S100 family of calcium-binding proteins. The core function of this antibody is to detect and measure the levels of S100A6 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti s100, by Abcam (2 mentions)
Anti map2 antibody, by Cell Signaling Technology (2 mentions)
Anti aldh1l1, by Merck Group (2 mentions)
Anti s100b, by Abcam (2 mentions)
Anti cd11b, by Thermo Fisher Scientific (2 mentions)
Anti cd68, by Cell Signaling Technology (2 mentions)
Paraformaldehyde aqueous solution, by Electron Microscopy Sciences (2 mentions)
Anti p tau antibody, by Thermo Fisher Scientific (2 mentions)
2 protocols using anti s100a6
1
Immunofluorescent Staining of Cells and 3D Cultures
2
Immunofluorescent Staining of Cells and 3D Cultures
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For immunofluorescent stains, we rinsed the cells and 3D cultures twice with PBS (phosphate buffered saline). Cells were then fixed through a RT 15–30 min incubation in fresh 4% paraformaldehyde aqueous solution (157–4, ElectronMicroscopy Sciences) followed by rinsing twice with PBS. Cells were permeabilized through incubation in 0.1% Triton X-100 in PBST (phosphate buffered saline with 0.1% tween®20) for 15 minutes at RT. Cell on-specific binding was blocked through overnight incubation in 3% human serum albumin in PBST at 4 °C. After a 24-hours incubation with the primary antibody solutions at 4°C, the cells were washed five times. The following antibodies (and dilutions) were used: anti-p-tau antibody (1:40, AT8, Thermo Scientific, MN1020); anti-PHF (1:1000, A gift from P. Davies, Albert Einstein College of Medicine); anti-GFAP antibody (1:500, Neuromap, N206A/8), anti-MAP2 antibody (1:200, Cell Signaling Technology, 4542); anti-CD68 (1:100, Cell Signaling, 76437); anti-cd11b (1:100, Life Technologies, NB110–89474); anti-S100 (1:400, Abcam, ab868); anti-S100A6 (1:200, Cell Signaling, D3H3W); anti-S100B (1:500, Abcam, Ab218515); anti-ALDH1L1 (1:200, EMD Millipore, MABN495).
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