Anti cd3 apc clone 17a2

Manufactured by Thermo Fisher Scientific

Anti-CD3-APC (clone 17A2) is a fluorescently labeled monoclonal antibody that binds to the CD3 antigen on T cells. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Facscanto, by BD (2 mentions) Collagenase, by Thermo Fisher Scientific (1 mentions) Anti cd45 pe clone 30 f11, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-CD3 (512 citations) CD3-APC (57 citations) Anti-CD3-APC (40 citations) CD3 (17A2, (21 citations) Anti-CD3 (17A2, (8 citations) Clone 17A2 (8 citations) Anti-CD3 (clone 17A2, (7 citations) CD3 (clone 17A2, (5 citations) CD3 APC (17A2, (2 citations)

2 protocols using anti cd3 apc clone 17a2

Lymphocyte Isolation and Characterization

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Secondary lymphoid organs were homogenized. Red blood cells were lysed for 5 min on ice in 150 mM NH₄Cl, 10 mM NaHCO₃, 100 µM Na₂-EDTA. Lymphocytes were washed in PBS 2% FCS and filtered on a nylon mesh. Cells were incubated with antiCD16/32 (Fc-block, clone 93, 1/100, eBiosciences) and stained with a mix of anti-CD19-PE.Cy7 (clone eBio1D3, 1/200, eBiosciences), anti-CD93-biot (mAb493, 1/100, prepared in-house by Antonius Rolink), anti-CD1d-FITC (clone 1B1, 1/100, eBiosciences), and anti-CD3-APC (clone 17A2, 1/100, eBiosciences) for 20 min at 4 °C followed by PE-Cy5.5-coupled streptavidin (1/200) and analysis with a FACS Canto (BD Biosciences). Data were analyzed with the FlowJo software.

Vigolo M., Chambers M.G., Willen L., Chevalley D., Maskos K., Lammens A., Tardivel A., Das D., Kowalczyk-Quintas C., Schuepbach-Mallepell S., Smulski C.R., Eslami M., Rolink A., Hummler E., Samy E., Fomekong Nanfack Y., Mackay F., Liao M., Hess H., Jiang X, & Schneider P. (2018). A loop region of BAFF controls B cell survival and regulates recognition by different inhibitors. Nature Communications, 9, 1199.

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Multiparametric Flow Cytometry Analyses

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Flow cytometry was performed on cell suspensions from the spleen, peripheral blood, thymus and lymph nodes. Mashing organs over 75 mm filters (BD) in RPMI plus 5% FBS provided single cell suspensions. Following red blood cell lysis using ACK (Ammonium-Chloride-Potassium) lysis buffer, the cells were stained for one hour on ice, washed with PBS and analyzed using a BD FACSCanto. The following antibodies were used for staining: Alexa488-conjugated 169 cDb, anti-CD4-PE (clone GK1.5), anti-CD45-APC (clone 30-F11; all fluorescent Abs from eBioscience).
For tumor digestion to single cell suspensions, tumors were incubated with Collagenase (type I; Invitrogen) at 1 mg/mL in RPMI plus 5% FBS for one hour at 37°C with continual shaking followed by straining over a 75 mm filter. The following antibodies were used for staining: anti-CD8-FITC (clone 53–6.7), anti-CD4-APC-Cy7 (clone GK1.5), anti-CD3-APC (clone 17A2), and anti-CD45-PE (clone 30-F11; all fluorescent Abs from eBioscience).

Tavaré R., Escuin-Ordinas H., Mok S., McCracken M.N., Zettlitz K.A., Salazar F.B., Witte O.N., Ribas A, & Wu A.M. (2015). An effective immuno-PET imaging method to monitor CD8-dependent responses to immunotherapy. Cancer research, 76(1), 73-82.

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