After CS exposure, HaCaT and A549 cells (1 x 106 cell/ml) were scraped and collected in 0.1M cacodylate buffer (pH 7.4), then spun in 1.5ml tubes at 2000×g for 5 min. Pellets were fixed with 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer for 4 h at 4 C. They were then washed with 0.1M cacodylate buffer (pH 7.4) three times and post-fixed in 1% osmium tetroxide and 0.1M cacodylate buffer at pH 7.4 for 1 h at room temperature. The specimens were dehydrated in graded concentrations of ethanol and embedded in epoxide resin (Agar Scientific, 66A Cambridge Road, Stanstead Essex, CM24 8DA, UK). Cells were then transferred to latex modules filled with resin and subsequently thermally cured at 60 C for 48 h. Semi-thin sections (0.5-1 m thickness) were cut using an ultra-microtome (Reichard Ultracut S, Austria) stained with toluidine blue, and blocks were selected for thinning. Ultra-thin sections of about 40-60 nm were cut and mounted onto formvar-coated copper grids. These were then double-stained with 1% uranyl acetate and 0.1% lead citrate for 30 min each and examined under a transmission electron microscope, Hitachi H-800 (Tokyo, Japan), at an accelerating voltage of 100 KV.
Epoxide resin
Manufactured by Agar Scientific
Sourced in United Kingdom
Epoxide resin is a type of synthetic polymer commonly used in various industrial and laboratory applications. It is a thermosetting material that cures through a chemical reaction, resulting in a rigid and durable material. Epoxide resin is known for its excellent adhesive properties, chemical resistance, and electrical insulation capabilities.
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Lab products found in correlation
2 protocols using epoxide resin
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Transmission Electron Microscopy of Cells
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Transmission Electron Microscopy of Cells
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Cells were scraped and collected in 0.1 M cacodylate buffer (pH 7.4) and then spun in 1.5 mL tubes at 2000 ×g for 5 min. Pellets were fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer for 4 hr at 4°C. They were then washed with 0.1 M cacodylate buffer (pH 7.4) three times and postfixed in 1% osmium tetroxide and 0.1 M cacodylate buffer at pH 7.4 for 1 hr at room temperature. The specimens were dehydrated in graded concentrations of ethanol and embedded in epoxide resin (Agar Scientific, 66A Cambridge Road, Stansted Essex CM24 8DA, UK).
Cells were then transferred to latex modules filled with resin and subsequently thermally cured at 60°C for 48 hr.
Semithin sections (0.5–1 μm thickness) were cut using an ultramicrotome (Reichard Ultracut S, Austria) stained with toluidine blue, and blocks were selected for thinning. Ultrathin sections of about 40–60 nm were cut and mounted onto formvar-coated copper grids. These were then double-stained with 1% uranyl acetate and 0.1% lead citrate for 30 min each and examined under a transmission electron microscope, Hitachi H-800 (Tokyo, Japan), at an accelerating voltage of 100 KV.
Cells were then transferred to latex modules filled with resin and subsequently thermally cured at 60°C for 48 hr.
Semithin sections (0.5–1 μm thickness) were cut using an ultramicrotome (Reichard Ultracut S, Austria) stained with toluidine blue, and blocks were selected for thinning. Ultrathin sections of about 40–60 nm were cut and mounted onto formvar-coated copper grids. These were then double-stained with 1% uranyl acetate and 0.1% lead citrate for 30 min each and examined under a transmission electron microscope, Hitachi H-800 (Tokyo, Japan), at an accelerating voltage of 100 KV.
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