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Mouse rat igf 1 elisa kit

Manufactured by R&D Systems
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The Mouse/Rat IGF-1 ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the in vitro determination of Insulin-like Growth Factor 1 (IGF-1) levels in mouse and rat samples. The kit utilizes a specific antibody coated on a microplate to capture IGF-1 from the samples, which is then detected using a secondary antibody conjugated to an enzyme. The enzymatic reaction produces a colorimetric signal that can be measured, allowing for the quantification of IGF-1 concentration.

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Lab products found in correlation

Milliplex map, by Merck Group (2 mentions) Mouse vegf elisa kit, by R&D Systems (2 mentions) Dlps 100, by BioAssay Systems (1 mentions) Rat mouse gh elisa kit, by Merck Group (1 mentions)

Close spelling variants of this product

IGF-1 ELISA kit Mouse IGF-1 Rat ELISA kit IGF-1 Mouse ELISA kits ELISAs

7 protocols using mouse rat igf 1 elisa kit

1

Hippocampal IGF-1 Concentration Measurement

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Hippocampal tissue was homogenized in NP-40 lysis buffer containing protease and phosphatase inhibitors. IGF-1 concentration was measured in the hippocampal homogenate using IGF-1 mouse/rat ELISA kit per manufacturer guidelines (cat# MG100, R&D Systems, Minneapolis, MN).
Pena G.S., Paez H.G., Johnson T.K., Halle J.L., Carzoli J.P., Visavadiya N.P., Zourdos M.C., Whitehurst M.A, & Khamoui A.V. (2020). Hippocampal Growth Factor and Myokine Cathepsin B Expression following Aerobic and Resistance Training in 3xTg-AD Mice. International Journal of Chronic Diseases, 2020, 5919501.
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2

Serum IGF-1, T4, and TSH Quantification

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Serum IGF-1 was detected using the IGF-1 Mouse/Rat ELISA kit (R&D Systems) following the manufacturer’s recommendations. Total plasma T4 levels were measured using a commercially available ELISA Kit (Diagnostic Automation/ Cortez Diagnostics, Inc Calabasas, CA). Thyroid-stimulating hormone (TSH) was measured in plasma via Milliplex MAP (mutianalyte panels) (mouse thyroid hormone TSH panel; EMD Millipore, Billerica, MA).
Hine C., Kim H.J., Zhu Y., Harputlugil E., Longchamp A., Matos M.S., Ramadoss P., Bauerle K., Brace L., Asara J.M., Ozaki C.K., Cheng S.Y., Singha S., Ahn K.H., Kimmelman A., Fisher F.M., Pissios P., Withers D.J., Selman C., Wang R., Yen K., Longo V.D., Cohen P., Bartke A., Kopchick J.J., Miller R., Hollenberg A.N, & Mitchell J.R. (2017). Hypothalamic-Pituitary Axis Regulates Hydrogen Sulfide Production. Cell Metabolism, 25(6), 1320-1333.e5.
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3

Serum IGF-1, T4, and TSH Quantification

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Serum IGF-1 was detected using the IGF-1 Mouse/Rat ELISA kit (R&D Systems) following the manufacturer’s recommendations. Total plasma T4 levels were measured using a commercially available ELISA Kit (Diagnostic Automation/ Cortez Diagnostics, Inc Calabasas, CA). Thyroid-stimulating hormone (TSH) was measured in plasma via Milliplex MAP (mutianalyte panels) (mouse thyroid hormone TSH panel; EMD Millipore, Billerica, MA).
Hine C., Kim H.J., Zhu Y., Harputlugil E., Longchamp A., Matos M.S., Ramadoss P., Bauerle K., Brace L., Asara J.M., Ozaki C.K., Cheng S.Y., Singha S., Ahn K.H., Kimmelman A., Fisher F.M., Pissios P., Withers D.J., Selman C., Wang R., Yen K., Longo V.D., Cohen P., Bartke A., Kopchick J.J., Miller R., Hollenberg A.N, & Mitchell J.R. (2017). Hypothalamic-Pituitary Axis Regulates Hydrogen Sulfide Production. Cell Metabolism, 25(6), 1320-1333.e5.
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4

Quantifying VEGF and IGF-1 in CDC Media

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Conditioned media was collected from twice-passaged CDCs 3 days after culture in 8-well chamber culture slides17 (link). A mouse VEGF ELISA kit and a mouse/rat IGF-1 ELISA kit (R&D Systems) were used to measure the levels of VEGF and IGF-1 in the conditioned media30 (link).
Luo L., Yan C., Urata Y., Hasan A.S., Goto S., Guo C.Y., Zhang S, & Li T.S. (2017). Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice. Scientific Reports, 7, 40959.
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5

Quantifying Secreted Factors in CSCs

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Conditioned media was collected from twice-passaged CSCs 3 days after culture in 8-well chamber culture slides. A mouse VEGF ELISA kit and a mouse/rat IGF-1 ELISA kit (R&D Systems) were used to measure the levels of VEGF and IGF-1 in the conditioned medium [18 (link)].
Luo L., Urata Y., Yan C., Hasan A.S., Goto S., Guo C.Y., Tou F.F., Xie Y, & Li T.S. (2016). Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice. PLoS ONE, 11(5), e0152179.
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6

Measurement of Serum FASN, Insulin, and IGF-1 in Xenograft Mice

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The serum FASN concentration of xenograft mice was measured in duplicate using a sandwich enzyme-linked immunosorbent assay (ELISA) kit (Uscn Life Science Inc., Houston, TX, USA). The kit was a human-specific ELISA that does not cross-react with mouse FASN. Serum insulin was measured using an insulin enzyme immunoassay kit (Morinaga Institute of Biological Science, Tokyo, Japan), and serum IGF-1 was measured using a mouse/rat IGF-1 ELISA kit (R&D Systems, Minneapolis, MN, USA). Briefly, 100 μl of standards and the diluted serum samples were incubated in the human FASN, mouse insulin or mouse IgG-coated 96-well plates for 2 h. After washing, a 1-h incubation period was performed with a second biotinylated anti-human FASN, anti-mouse insulin or anti-mouse IGF-1 antibody and followed by a 30-min incubation period with streptavidin peroxidase (horseradish peroxidase). After washing to remove all unbound enzyme, colour was generated by adding tetramethylbenzidine and the reaction was stopped with a stop solution (2 N H2SO4). Concentrations of serum FASN, insulin and serum IGF-1 were calculated using standard curves.
Huang M., Koizumi A., Narita S., Inoue T., Tsuchiya N., Nakanishi H., Numakura K., Tsuruta H., Saito M., Satoh S., Nanjo H., Sasaki T, & Habuchi T. (2016). Diet-induced alteration of fatty acid synthase in prostate cancer progression. Oncogenesis, 5(2), e195-.
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7

Serum Biomarker Profiling in Rodents

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ELISA was performed to detect serum GH (rat/mouse GH ELISA kit; Merck KGaA), IGF-1 (mouse/rat IGF-1 ELISA kit; R&D Systems), and total thyroxine (total thyroxine ELISA; ALPCO) according to the protocols of the respective ELISA kits. Serum amylase and lipase activities were measured using kits from BioAssay Systems (ECAM-100 and DLPS-100) according to the manufacturer's instructions.
Wei W., Liu Z., Zhang C., Khoriaty R., Zhu M, & Zhang B. (2021). A common human missense mutation of vesicle coat protein SEC23B leads to growth restriction and chronic pancreatitis in mice. The Journal of Biological Chemistry, 298(1), 101536.
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