Potassium chloride (kcl)

Polycaprolactone (MW 80,000, Aldrich, St. Louis, MO, USA) (PCL), formic acid (88%, PQM, NL., Mex), acetic acid (99.7%, Aldrich, St. Louis, MO, USA), acetone (99%, CTR Scientific, N.L., Méx.), chloroform (98%, Sigma-Aldrich, St. Louis, MO, USA that was used as a solvent for PCL), and nanoparticles of TiO₂ (≥99.5%) and silver (Ag) (99.5%, Sigma-Aldrich, St. Louis, MO, USA) were used as antibacterial particles. Na₂Ti₆O₁₃ particles were synthesized by a sol–gel method as is described in previous work [41 ].
For bioactivity assays: sodium chloride (Aldrich, Saint Louis, MO, USA), sodium hydrogen carbonate (Aldrich, Saint Louis, MO, USA), potassium chloride (VETEC, Saint Louis, MO, USA), dipotassium hydrogen phosphate (Aldrich, Saint Louis, MO), magnesium chloride hexahydrate (Aldrich, Saint Louis, MO, USA), calcium chloride dihydrate (Aldrich, Saint Louis, MO, USA), sodium sulfate (Aldrich, Saint Louis, MO, USA), hydrochloric acid and tris(hydroxymethyl) aminomethane (Aldrich, Saint Louis, MO, USA) were used.
The cells NIH/3T3 ATCC ^® CRL-1658™ were cultured in normal medium (DMEM F-12), 5% FBS, 1% antibiotics (DIFCO Gibco^® Life Technologies and Sigma-Aldrich, St. Louis, MO, USA). The gram-positive bacterial strain was Staphylococcus aureus (ATCC 25923).

Potassium chloride (KCl), calcium chloride (CaCl₂), magnesium chloride (MgCl₂), sodium chloride (NaCl), and formaldehyde were purchased from Vetec Química Fina Ltda. (Brazil). Sodium bicarbonate (NaHCO₃) and glucose (C₆H₁₂O₆) were purchased from Dinâmica (Brazil). Sodium monobasic phosphate (NaH₂PO₄), sodium hydroxide (NaOH), and hydrochloric acid (HCl) were purchased from Nuclear (Brazil). These substances, except glucose, NaCl, and NaHCO₃, were diluted in distilled water to obtain each solution, which was maintained under refrigeration.
Carbamylcholine hydrochloride (CCh) was purchased from Merck (USA). Cremophor®, thiobarbituric acid, tetramethoxypropane, perchloric acid, Mayer's hematoxylin, and eosin were acquired from Sigma-Aldrich (Brazil). All substances were diluted in distilled water as needed for each experimental protocol. The carbogen mixture (95% O₂ and 5% CO₂) was obtained from White Martins (Brazil).

Chemicals: Agar Müeller Hinton and Saboraud Dextrose Agar and Broth were purchased from HiMedia^® (Mumbai, India). Amphotericin B (AmB) was obtained from Infect Chemphar CO. (Hong Kong, China). Dimethyl sulfoxide (DMSO) 99.9% A.C.S spectrophotometric grade, Tween^® 80 and sodium phosphate dibasic were provided from Sigma-Aldrich (Saint Louis, MO, USA). The colorless nail lacquer was purchased from Ideal Cosméticos (Nova Lima, Brazil). Anhydrous monobasic potassium phosphate and glycerol came from Synth (São Paulo, Brazil). Sodium chloride and potassium chloride were provided from Vetec (Duque de Caxias, Brazil) and QEEL (São Paulo, Brazil), respectively.
Microorganisms: The four American Type Culture Collection strains of Candida spp. (Candida albicans ATCC 90028, C. glabrata ATCC 2001, C. tropicalis ATCC 13803 and C. parapsilosis ATCC 22019) and one Centraalbureau voor Schimmelcultures (C. dubliniensis CBS 7987) were donated by the culture collection of the Laboratory of Medical and Molecular Mycology from the Federal University of Rio Grande do Norte (UFRN, Natal, Brazil). The microorganisms were maintained in Saboraud Dextrose Broth containing 20% glycerol, frozen at −80 °C until the moment of the experiment. All stored fungi were cultured in Saboraud Dextrose Agar for 48 h at 37 °C before the experiments.