Dig labeled 11 utp

Manufactured by Roche

Sourced in Switzerland

DIG)-labeled 11-UTP is a nucleotide analog used in various molecular biology techniques. It features a digoxigenin (DIG) label attached to the 11th position of the uridine triphosphate (UTP) molecule. This labeled nucleotide can be incorporated into nucleic acid probes or RNA transcripts for detection and visualization purposes.

Automatically generated - may contain errors

Lab products found in correlation

Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

DIG-11-UTP DIG-labeled UTP DIG-UTP

2 protocols using dig labeled 11 utp

Antisense Probe Generation from cDNA Clones

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Antisense probes were generated from commercial mouse cDNA clones (Source BioScience). The clones were sequenced (Eurofins MWG Operon, Germany) and verified to be correct. Plasmids were linearized with appropriate restriction enzymes and the probes were synthesized using 1μg vector as template with T7, Sp6 or T3 RNA polymerase in the presence of digoxigenin (DIG)-labeled 11-UTP (Roche Diagnostics, Switzerland). Probes were controlled and quantified using the Nanodrop ND-1000 Spectrophotometer (NanoDrop Technologies, Delaware, USA).

Williams M.J., Klockars A., Eriksson A., Voisin S., Dnyansagar R., Wiemerslage L., Kasagiannis A., Akram M., Kheder S., Ambrosi V., Hallqvist E., Fredriksson R, & Schiöth H.B. (2016). The Drosophila ETV5 Homologue Ets96B: Molecular Link between Obesity and Bipolar Disorder. PLoS Genetics, 12(6), e1006104.

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Generation of Digoxigenin-Labeled RNA Probes

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Antisense probes were generated from commercial mouse cDNA clones (Source BioScience). The clones were sequenced (Eurofins MWG Operon, Germany) and verified to be correct. Plasmids were linearized with appropriate restriction enzymes and the probes were synthesized using 1 μg vector as template with T7, Sp6 or T3 RNA polymerase in the presence of digoxigenin (DIG)-labeled 11-UTP (Roche Diagnostics, Switzerland). Probes were controlled and quantified using the Nanodrop ND-1000 Spectrophotometer (NanoDrop Technologies, USA).

Eriksson A., Williams M.J., Voisin S., Hansson I., Krishnan A., Philippot G., Yamskova O., Herisson F.M., Dnyansagar R., Moschonis G., Manios Y., Chrousos G.P., Olszewski P.K., Frediksson R, & Schiöth H.B. (2015). Implication of coronin 7 in body weight regulation in humans, mice and flies. BMC Neuroscience, 16, 13.

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