G2 f20 twin transmission electron microscope

Manufactured by Thermo Fisher Scientific

The G2 F20-TWIN Transmission Electron Microscope is a high-performance instrument designed for advanced imaging and analysis of samples at the nanoscale. It features a twin-lens system that provides exceptional resolution and contrast for a wide range of applications.

Automatically generated - may contain errors

Lab products found in correlation

Vitrobot mark 4, by Thermo Fisher Scientific (5 mentions) C flat holey carbon grid, by Protochips (4 mentions) Cryo holder model 626 70, by Ametek (2 mentions) 16 megapixel ccd camera, by Ametek (1 mentions) 4k 4k ccd camera, by Ametek (1 mentions) 4k x 4k ccd camera, by Ametek (1 mentions) Pelco easiglow, by Ted Pella (1 mentions) Titan krios electron microscope, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Tecnai G2 F20 X-TWIN Transmission Electron Microscope (TEM)) FEI Tecnai G2 F20 S-TWIN transmission electron microscope Tecnai G2 F20-TWIN transmission electron microscope Tecnai G2 F20 X-TWIN transmission electron microscope Tecnai G2 F20 S-TWIN transmission electron microscope F20 electron microscope Transmission electron microscope F20 microscope

5 protocols using g2 f20 twin transmission electron microscope

Vitrification of Liposome-Oligonucleotide Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Liposomes incorporated with oligonucleotides were reconstituted with PBS
at 1 mg/mL. Three-microliter aliquots were applied to C-flat holey carbon grids
(Protochips, Inc. Morrisville, NC) and vitrified using a VitrobotTM Mark IV (FEI
Co., Hillsboro, OR). The sample was examined using a 16-megapixel CCD camera
(Gatan Inc., Pleasanton, CA) in a Tecnai (FEI Co.) G2 F20-TWIN Transmission
Electron Microscope operated at a voltage of 200 kV using low dose conditions
(~20 e/Å2). Images were recorded with a defocus of approximately 3
µm to improve contrast.

Ashizawa A.T., Holt J., Faust K., Liu W., Tiwari A., Zhang N, & Ashizawa T. (2019). Intravenously Administered Novel Liposomes, DCL64, Deliver Oligonucleotides to Cerebellar Purkinje Cells. Cerebellum (London, England), 18(1), 99-108.

+ Open protocol

+ Expand

Cryo-TEM Virus Sample Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sample preparation for cryo-transmission electron microscopy (cyro-TEM) was performed in the EM Core of the University of Florida’s Interdisciplinary Center for Biotechnology Research. Three microliter aliquots of suspended virus were applied to C-flat holey carbon grids (Protochips, Inc.) and vitrified using a Vitrobot™ Mark IV (FEI Co.) operated at 4°C and with ~90% humidity in the control chamber. The vitrified sample was stored under liquid nitrogen and transferred into a Gatan cryo-holder (Model 626/70) for imaging. The sample was examined using a 4k × 4k CCD camera (Gatan, Inc.) on a Tecnai (FEI Co.) G2 F20-TWIN Transmission Electron Microscope operated at a voltage of 200 kV using low dose conditions (~20 e/Å2). Images were recorded with a defocus of approximately 3 μm to improve contrast.

Brice D.C., Toth Z, & Diamond G. (2018). LL-37 Disrupts the Kaposi’s Sarcoma-Associated Herpesvirus Envelope and Inhibits Infection in Oral Epithelial Cells. Antiviral research, 158, 25-33.

+ Open protocol

+ Expand

Cryogenic TEM Imaging of Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Three microliter aliquots of the NV were applied to C-flat holey carbon grids (Protochips, Inc.) and vitrified using a Vitrobot™ Mark IV (FEI Co.) operated at 4°C and with ~90% humidity in the control chamber. The vitrified sample was stored under liquid nitrogen and transferred into a Gatan cryo-holder (Model 626/70) for imaging. The sample was examined using a 4k x 4k CCD camera (Gatan, Inc.) on a Tecnai (FEI Co.) G2 F20-TWIN Transmission Electron Microscope operated at a voltage of 200 kV using low dose conditions (~20 e/Å2). Images were recorded with a defocus of approximately -2µm to improve contrast.

Harrison N.A., Gardner C.L., da Silva D.R., Gonzalez C.F, & Lorca G.L. (2021). Identification of Biomarkers for Systemic Distribution of Nanovesicles From Lactobacillus johnsonii N6.2. Frontiers in Immunology, 12, 723433.

+ Open protocol

+ Expand

Cryogenic Transmission Electron Microscopy of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated EVs were re-suspended in 1X PBS buffer solution. To disperse any aggregated EVs, the sample was sonicated for 30 s immediately prior to freezing. Sample preparation for Cryo-TEM was performed in the EM Core of the University of Florida’s Interdisciplinary Center for Biotechnology Research. Three microlitre aliquots of suspended EVs sample were applied to C-flat holey carbon grids (Protochips, Inc.) and vitrified using a Vitrobot™ Mark IV (FEI Co.) operated at room temperature and with 100% humidity in the control chamber. The vitrified sample was stored under liquid nitrogen to avoid ice crystals formation and transferred into a Gatan cryo holder (Model 626/70) for imaging. The sample was examined using a 16-megapixel CCD camera (Gatan, Inc.) on a Tecnai (FEI Co.) G2 F20-TWIN Transmission Electron Microscope operated at a voltage of 200 kV using low dose conditions (~20 e/Å²). Images were recorded with a defocus of approximately 3 µm to improve contrast.

Sutaria D.S., Jiang J., Elgamal O.A., Pomeroy S.M., Badawi M., Zhu X., Pavlovicz R., Azevedo-Pouly A.C., Chalmers J., Li C., Phelps M.A, & Schmittgen T.D. (2017). Low active loading of cargo into engineered extracellular vesicles results in inefficient miRNA mimic delivery. Journal of Extracellular Vesicles, 6(1), 1333882.

+ Open protocol

+ Expand

Cryo-EM Imaging of AAVhum.8 Vectors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quantifoil grids with a thin carbon film over the holes were glow discharged in a Pelco easiGlow (Ted Pella, Inc., Redding, CA) for 30 s prior to sample loading. Small volumes of purified AAVhum.8 vectors (∼3 μl/grid) were loaded onto the prepared grids and vitrified in liquid ethane by a Vitrobot Mark IV (FEI, Hillsboro, OR) freezing robot and subsequently transferred into liquid nitrogen. The capsid distribution and ice quality of the grids were examined using a 16-megapixel CCD camera (Gatan, Inc.) in a Tecnai (FEI Co.) G2 F20-TWIN transmission electron microscope operated at a voltage of 200 kV using low-dose conditions (∼20 e/Å²). Optimal grids were used for collecting micrograph movie frames using the Leginon semiautomated application (53 (link)) on a Titan Krios electron microscope (FEI Co.) operated at 300 kV at a total dose of 60 e⁻/Å² recorded on a Gatan K3 direct electron detection camera with 50 movie frames per micrograph. The data were collected at the Biological Science Imaging Resource of the Florida State University as part of the NIH Southeastern Center for Microscopy of MacroMolecular Machines (SECM4) project.

Havlik L.P., Das A., Mietzsch M., Oh D.K., Ark J., McKenna R., Agbandje-McKenna M, & Asokan A. (2021). Receptor Switching in Newly Evolved Adeno-associated Viruses. Journal of Virology, 95(19), e00587-21.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!